Hsa_circ_0000423 promotes colorectal cancer EMT and immune escape by competitive adsorption of miR-369-3p mediating CCND1 expression by TianFu Huang, KaiHai Jiang, LinTao Li, GuangSheng Li, YuSheng Cao, XuSen Huang

“…Methods The hsa_circ_0000423 gene was identified by bioinformatics analyses of GEO circRNA microarrays, and its expression in CRC was investigated. …”
Get full text

RPS21 Enhances hepatocellular carcinoma development through GPX4 stabilization by Siyuan Wu, Gaochao Wang, Likai Gu, Yinjie Zhang, Zhihuai Wang

“…Mutation analysis via the cBioPortal showed that primarily amplifications in RPS21 are associated with a poorer prognosis. Tissue microarrays confirmed higher RPS21 levels in tumor samples, which were associated with more advanced clinical stages and grades. …”
Get full text

Gene Expression Analysis and Validation of a Novel Biomarker Signature for Early-Stage Lung Adenocarcinoma by Sanjan S. Sarang, Catherine M. Cahill, Jack T. Rogers

“…Using 22 pairs of LUAD and matched normal lung microarrays, 229 differentially expressed genes were identified. …”
Get full text

Nonlinear Quantitative Radiation Sensitivity Prediction Model Based on NCI-60 Cancer Cell Lines by Chunying Zhang, Luc Girard, Amit Das, Sun Chen, Guangqiang Zheng, Kai Song

“…Important radiation therapy (RT) related genes were selected by significance analysis of microarrays (SAM). Orthogonal latent variables (LVs) were then extracted by the partial least squares (PLS) method as the new compressive input variables. …”
Get full text

New Frontiers for the Early Diagnosis of Cancer: Screening miRNAs Through the Lateral Flow Assay Method by Afsaneh Emamiamin, Seyedeh Rojin Shariati Pour, Thea Serra, Donato Calabria, Marta Varone, Fabio Di Nardo, Massimo Guardigli, Laura Anfossi, Claudio Baggiani, Martina Zangheri, Mara Mirasoli

“…However, current methods for miRNA quantification, such as northern blotting, isothermal amplification, RT-PCR, microarrays, and next-generation sequencing, are limited by their reliance on centralized laboratories, high costs, and the need for specialized personnel. …”
Get full text

MaGIC: a program to generate targeted marker sets for genome-wide association studies by Claire L. Simpson, Valerie K. Hansen, Pak C. Sham, Andrew Collins, John F. Powell, Ammar Al-Chalabi

“…High-throughput genotyping technologies such as DNA pooling and DNA microarrays mean that whole-genome screens are now practical for complex disease gene discovery using association studies. …”
Get full text

Lactoferrin-osteopontin complexes: insights into intestinal organoid bioavailability and gut microbiota modulation by Camilla Ceroni, Loïc Dayon, Pieter Van den Abbeele, Aline Roch, Juliane Dervaux, Marion Ernest, Pascaline Sirugue, Aurélien Baudot, Marine R.-C. Kraus, Nathalie Brandenberg, Jonathan O’Regan, David Goulding, Omid Mashinchian

“…We investigated the bioavailability and intestinal uptake dynamics of bovine LF-OPN soluble complexes (SC) and complex coacervates (CC) using a microarrayed high-throughput 3D apical-out intestinal organoid platform, which closely mimics the human intestinal epithelium. …”
Get full text

The DCDC2/ENO1 axis promotes tumor progression and immune evasion in intrahepatic cholangiocarcinoma via activating FGL1-LAG3 checkpoint by Wenze Wan, Yuan Li, Wentao Sun, Zewei Cheng, Fen Ma, Sheng Shen, Houbao Liu, Jiwei Zhang

“…Our study included protein microarrays, transcriptome analysis, functional assays, immunofluorescence, dual-luciferase reporter assays, as well as xenograft models and humanized PBMC models. …”
Get full text

Sarcomatoid areas of urothelial carcinoma are enriched for CD163‐positive antigen‐presenting cells by Burles A Johnson III, Vamsi Parimi, Sonia Kamanda, David C Corney, Woonyoung Choi, Jean Hoffman‐Censits, Max Kates, David J McConkey, Noah M Hahn, Andres Matoso

“…The objective of this study was to identify differences between the sarcomatoid and CUC tumor microenvironment components that may drive this aggressive phenotype. Using tissue microarrays from eight patient tumors with mixed CUC and SUC, we examined paired CUC, mixed urothelial carcinoma (UC) regions, and SUC using the Nanostring Digital Spatial Profiling platform. …”
Get full text

Patient-based transcriptome-wide analysis identify interferon and ubiquination pathways as potential predictors of influenza A disease severity. by Long Truong Hoang, Thomas Tolfvenstam, Eng Eong Ooi, Chiea Chuen Khor, Ahmand Nazri Mohamed Naim, Eliza Xin Pei Ho, Swee Hoe Ong, Heiman F Wertheim, Annette Fox, Chau Van Vinh Nguyen, Ngoc My Nghiem, Tuan Manh Ha, Anh Thi Ngoc Tran, Paul Tambayah, Raymond Lin, Chariya Sangsajja, Weerawat Manosuthi, Chareon Chuchottaworn, Piamlarp Sansayunh, Tawee Chotpitayasunondh, Piyarat Suntarattiwong, Kulkanya Chokephaibulkit, Pilaipan Puthavathana, Menno D de Jong, Jeremy Farrar, H Rogier van Doorn, Martin Lloyd Hibberd

“…<h4>Methodology/principal findings</h4>Using gene expression microarrays, the transcriptomic profiles of influenza-infected patients with severe (N = 11), moderate (N = 40) and mild (N = 83) symptoms were compared with the febrile patients of unknown etiology (N = 73). …”
Get full text

The expression and prognosis for Aurora kinases in human non-small cell lung cancer by Yue Wang, Juan Liu, Jiaxue Xu, Zhaodong Ji

“…Immunohistochemistry (IHC) was performed on tissue microarrays (TMAs) from 29 ‌lung adenocarcinoma (LUAD) patients. …”
Get full text

Downregulation of AKT-mediated p27Kip1 phosphorylation with shift to sphingomyelin synthesis in CLN3 disease by Fatima Bilal, Jihane Soueid, Sara Saab, Nadine Makhoul, Zeinab Hamze, Lara El-Bazzal, Joelle Makoukji, Rose-Mary Boustany

“…Transcriptomic analysis of gene expression using microarrays, validation by Western blot and real-time PCR, demonstrate that AKT is downregulated in CLN3-deficient cells and Cln3Δex7/8 mouse brain. …”
Get full text

VPS25 Promotes an Immunosuppressive Microenvironment in Head and Neck Squamous Cell Carcinoma by Li-Guo Chen, Yu-Han Fang, Kui-Ming Wang, Wei Zhang, Gang Chen

“…Bulk RNA-sequencing data and HNSCC tissue microarrays (TMAs) were used to evaluate VPS25 expression and its clinical significance. …”
Get full text

Inhibition of PDK modulates radiotherapy resistance in gastric cancer by Qi Zhang, Wanjia Qiao, Xiaoyu Liu, Jun Lu, Farooq Benish, Shuping Li, Xiaojun Liu

“…Abstract To explore the effect of sodium dichloroacetate (DCA), a pyruvate dehydrogenase kinase (PDK) inhibitor, on the progression of gastric cancer and resistance to radiotherapy, we analyzed histopathological microarrays from 60 gastric cancer and paracancerous tissues to determine PDK expression and its prognostic significance. 5-Ethynyl-2′-deoxyuridine (EdU) incorporation assay and Transwell migration assay were used to investigate the effects of PDK inhibition on gastric cancer cell proliferation and migration. …”
Get full text

Distribution of Recessive Genetic Defect Carriers in Holstein Friesian Cattle: A Polish Perspective by Marta Gozdek, Sebastian Mucha, Adam Prostek, Dariusz Kamola, Tomasz Sadkowski

“…The studies were performed using Illumina Infinium microarrays. Among both bulls and cows, the highest numbers of carriers were detected for HH5 (appropriately 6.7% and 5.4%). …”
Get full text

Re-evaluation of the relationship between PrPc expression and patient prognosis in primary esophageal squamous cell carcinoma and primary hepatocellular carcinoma by Cheng Zhang, Fanlei Ran, Lei Du, Yang Cao, Hong Chen, Quan Chen, Lijun Bi, Haiying Hang

“…Immunohistochemistry with 4AA-m was then performed on tissue microarrays from 179 patients with primary ESCC and 92 patients with primary HCC. …”
Get full text

Classification of gliomas based on genome-wide DNA methylation profiles and immunohistochemical analysis: a short study by Olga Taryma-Leśniak, Kamil Buczkowski, Tomasz Klepinowski, Maciej Jankowski, Szymon Retfiński, Bartłomiej Sagan, Leszek Sagan, Ewa Szutowicz, Kacper Winiarski, Marta Masztalewicz, Przemysław Nowacki, Marta Sobalska-Kwapis, Dominik Strapagiel, Wojciech Kloc, Beata Lipska-Ziętkiewicz, Ewa Iżycka-Świeszewska, Tomasz K. Wojdacz

“…The formalin-fixed paraffin-embedded (FFPE) and freshly frozen (FF) samples were analyzed using Infinium Methylation-EPIC microarrays. The diagnosis was changed in two cases, and two other cases were classified as control tissue due to the low content of neoplastic cells in examined frozen samples. …”
Get full text

Prognostic Significance of Tissue Inhibitor of Metalloproteinase-1 in Breast Cancer by Arunee Dechaphunkul, Monlika Phukaoloun, Kanet Kanjanapradit, Kathryn Graham, Sunita Ghosh, Cheryl Santos, John R. Mackey

“…Primary breast cancers from women with early stage disease treated with standard adjuvant therapy were analyzed by gene expression microarrays and immunohistochemistry for TIMP-1. Results. …”
Get full text

Pan-cancer landscape of ITGAV and its potential role in gastric cancer by Bin Ke, Peng Jin, Xue-Jun Wang, Ru-peng Zhang, Ning Liu, Gang Ma

“…Gene enrichment analysis was performed to explore potential functions of ITGAV in gastric cancer (GC). GC tissue microarrays and in vitro cell experiments were used to verify the prediction results in GC. …”
Get full text

Targeting Mesothelin Enhances Personalized Neoantigen Vaccine Induced Antitumor Immune Response in Orthotopic Pancreatic Cancer Mouse Models by Zhixiong Cai, Zhenli Li, Wenting Zhong, Fangzhou Lin, Xiuqing Dong, Honghao Ye, Yutong Guo, Geng Chen, Xiaoling Yu, Haijun Yu, Ruijing Tang, Xiaolong Liu

“…Here, three datasets and multi‐immunofluorescence staining of tissue microarrays in pancreatic cancer indicate that mesothelin (MSLN) expression negatively correlates with cytotoxic T cells in tumor. …”
Get full text