Exploring miR-30b Methylation and MALAT-1 Expression as Diagnostic Biomarkers for Non-Small Cell Lung Cancer by Marziyeh Memarzadeh, Habib Zarredar, Milad Asadi, Armin Sadeghi, Venus Zafari, Shahryar Hashemzadeh, Hamed Sabbagh-Jadid, Mortaza Raeisi

“…This study aimed to investigate miR-30b DNA methylation and metastasis associated lung adenocarcinoma transcript 1 (MALAT-1) expression patterns as potential diagnostic biomarkers for non-small cell lung cancer (NSCLC).Method: In this cross-sectional study, miR-30b DNA methylation and MALAT-1 expression patterns were first explored using microarray data retrieved from the NSCLC dataset in the Cancer Genome Atlas (TCGA)-LUNG. …”
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Identifying 4 Novel lncRNAs as Potential Biomarkers for Acute Rejection and Graft Loss of Renal Allograft by Zedan Zhang, Yanlin Tang, Hongkai Zhuang, Enyu Lin, Lu Xie, Xiaoqiang Feng, Jiayi Zeng, Yanjun Liu, Jiumin Liu, Yuming Yu

“…This study was performed to identify novel long noncoding RNAs (lncRNAs) for diagnosing AR and predicting the risk of graft loss. The several microarray datasets with AR and nonrejection specimens of renal allograft downloaded from Gene Expression Omnibus database were analyzed to screen differentially expressed lncRNAs (DElncRNAs) and mRNAs (DEmRNAs). …”
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Discovery of a novel highly specific, fully human PSCA antibody and its application as an antibody-drug conjugate in prostate cancer by Xiaojie Chu, Seungmin Shin, Du-San Baek, Liyong Zhang, Alex Conard, Megan Shi, Ye-Jin Kim, Cynthia Adams, Maggie Hines, Xianglei Liu, Chuan Chen, Zehua Sun, Dontcho V. Jelev, John W. Mellors, Dimiter S. Dimitrov, Wei Li

“…F12 targets PSCA amino acids 63–69 as tested by the peptide scanning microarray, and it cross-reacts with the murine PSCA. …”
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Genetic correlation between fetal nuchal translucency thickening and cystic hygroma and exploration of pregnancy outcome by Jianli Zheng, Tiantian Wang, Huilin Sun, Yongjuan Guan, Fangfang Yang, Jing Wu, Feifei Ying, Yadong Fu, Min Li, Jianbing Liu

“…Abstract Chromosome microarray analysis (CMA) and whole exome sequencing (WES) are increasingly utilized in prenatal diagnosis of abnormal ultrasound findings, but studies on correlation between pathogenic copy number variations (pCNVs) and single-gene mutations in fetuses with nuchal translucency (NT) thickening/cystic hygroma (CH), and pregnancy outcomes, are rare. …”
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First Prenatal Case of Genotypically and Phenotypically Overlapping Double Molecular Diagnosis of Van den Ende–Gupta and 22q11.2 Deletion Syndromes by Caroline Racine, Aurore Garde, Olivia Martz, Hana Safraou, Vinciane Eluard, Thierry Rousseau, Nathalie Marle, Fara T. Harizay, Laurent Martin, Julien Maraval, Ange‐Line Bruel, Christophe Philippe, Christel Thauvin‐Robinet, Laurence Faivre

“…Methods An unrelated couple was referred at 21 + 3 weeks of gestation for talipes equinovarus, cerebellar hypoplasia, clenched fists, elevated hemidiaphragm, and micrognathia. Chromosomal microarray and exome sequencing analyses were performed. …”
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Integrated analysis of genomic and transcriptomic profiles identified a prognostic immunohistochemistry panel for esophageal squamous cell cancer by Yue Yu, Zhihua Li, Chenjun Huang, Haisheng Fang, Fei Zhao, Yue Zhou, Xianglong Pan, Qifan Li, Yu Zhuang, Liang Chen, Jing Xu, Wei Wang

“…Methods An integrated analysis was performed to screen and establish a prognostic panel using exome sequencing profile from 81 pairs of ESCC samples and RNA expression microarray data from 119 ESCC subjects. Two independent ESCC cohorts were recruited as training and validation groups to test the prognostic value. …”
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Mechanism of immune escape mediated by receptor tyrosine kinase KIT in thyroid cancer by Zhen Luo, Jin Xu, Dayong Xu, Jiaojiao Xu, Rongjun Zhou, Keping Deng, Zheng Chen, Fang Zou, Libo Yao, Yuqin Hu

“…Methods The expression microarray of TC was acquired through the GEO database, and the difference analysis and Kyoto encyclopedia of genes and genomes pathway enrichment analysis were carried out. …”
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Ultrasensitive Peptide-Based Electrochemical Biosensor for Universal Diagnostic of Dengue by Isis Campos Prado, João Pedro Rangel da Silva Carvalho, André Souza Araujo, Paloma Napoleão-Pêgo, Salvatore Giovanni De-Simone

“…An identified linear B-cell epitope (DENV/18) specific for DENV 1–4 serotypes recognized by IgG in patient sera was selected as a target molecule after a microarray of peptides using the SPOT-synthesis methodology. …”
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Bioinformatic and Phenotypic Analysis of <i>AtPCP-Ba</i> Crucial for Silique Development in <i>Arabidopsis</i> by Guangxia Chen, Xiaobin Wu, Ziguo Zhu, Tinggang Li, Guiying Tang, Li Liu, Yusen Wu, Yujiao Ma, Yan Han, Kai Liu, Zhen Han, Xiujie Li, Guowei Yang, Bo Li

“…In this study, we identified many CRP genes downstream of the CRP gene <i>TPD1 (TAPETUM DETERMINANT1)</i> during silique development using a microarray assay. The novel <i>Arabidopsis thaliana</i> pollen-borne CRPs, the PCP-Bs (for pollen coat protein B-class) gene <i>AtPCP-Ba</i>, along with <i>TPD1</i>, are essential for silique development. …”
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Targeting FGFR4 abrogates HNF1A-driven metastasis in pancreatic ductal adenocarcinoma by Katherine J. Crawford, Kennedy S. Humphrey, Eduardo Cortes, Jianxin Wang, Vishnu Kumarasamy, Yin Wan, Mark D. Long, Michael E. Feigin, Agnieszka K. Witkiewicz, Karen M. Mann, Erik S. Knudsen, Ethan V. Abel

“…Single-cell RNA sequencing (scRNA-seq), tissue microarray (TMA) data, and UMAP spatial profiling were used to identify FGFR4 as an HNF1A target gene upregulated in metastatic cells. …”
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The differentially expressed circular ribonucleic acids of primary hepatic carcinoma following liver transplantation as new diagnostic biomarkers for primary hepatic carcinoma by Weiguo Sui, Qing Gan, Fuhua Liu, Huaizhou Chen, Junfu Liu, Yong Dai

“…We chose differentially expressed targeted circular ribonucleic acids according to fold change ≥2.0 or ≤–2.0 between circular ribonucleic acids microarray of perioperative liver transplantation and normal controls. …”
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Multiple myeloma with extramedullary plasmacytoma: pathogenesis and clinical case by M. V. Firsova, N. V. Risinskaya, M. V. Solovev, T. N. Obukhova, M. A. Kislitsyna, E. E. Nikulina, I. A. Yakutik, T. V. Abramova, A. B. Sudarikov, A. M. Kovrigina, L. P. Mendeleeva

“…Comparative genomic hybridization on a microarray (arrayCGH) plasmacytoma dNA was also performed. …”
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A 22 month prospective assessment of neutralizing and IgG antibody levels against SARS-CoV-2 variants following homologous and heterologous BNT162b2 boosting by Aini Syahida Mat Yassim, Anis Atifah Mohd Hisham, Nik Nur Atiqah Nik Daud, Nur Diana Anuar, Ti-Myen Tan, Rapeah Suppian, Mat Jusoh Siti Asmaa, Amiratul Aifa Mohamad Asri, Maryam Azlan, Nur Suhaila Idris, Rosediani Muhamad, Mohd Nor Norazmi

“…Using the ImmuSAFE™ COVID + microarray, we evaluated IgG-S and NAb against Wuhan-Hu and VoCs (Alpha, Beta, Delta, Omicron), as well as IgG-N responses to Wuhan-Hu. …”
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Identification of FABP5 as an immunometabolic marker in human hepatocellular carcinoma by Yan Lu, Shuang Zhou, Han Wang, Fangming Liu, Weiren Liu, Chunhui Yang, Mengxin Tian, Guangshuai Jia, Bijun Zhu, Mingxiang Feng, Tiankui Qiao, Xinxin Wang, Wei Cao, Xiangdong Wang, Yinghong Shi, Duojiao Wu

“…Immunohistochemistry staining on the tissue microarray of 118 patients with HCC showed intra-tumoral FABP5 high CD8+ T-cell infiltration was linked to overall and recurrence-free survival.Conclusions The tumor microenvironment can impose metabolic restrictions on T-cell function. …”
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Different Angiogenic Potentials of Mesenchymal Stem Cells Derived from Umbilical Artery, Umbilical Vein, and Wharton’s Jelly by Lu Xu, Jianjun Zhou, Jingyu Liu, Yong Liu, Lei Wang, Ruiwei Jiang, Zhenyu Diao, Guijun Yan, Bruno Pèault, Haixiang Sun, Lijun Ding

“…Additionally, the total tube length in UCA-PSCs and UCV-PSCs was significantly longer than in WJ-MSCs (P<0.01). Microarray, qRT-PCR, and Western blot analysis showed that UCA-PSCs had the highest expression of the Notch ligand Jagged1 (JAG1), which is crucial for blood vessel maturation. …”
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Sequential prenatal diagnosis of fetal skeletal dysplasia: A cohort study by Mengting Jiang, Bin Zhang, Jing Wang, Wei Qiao, Xiuzhen Mao, Bin Yu

“…Abstract Introduction Genetic factors are considered to be the main factors leading to fetal skeletal dysplasia (SD), and chromosomal microarray analysis (CMA) has been used clinically for the detection of SD fetuses. …”
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Editorial by Editorial

“…Terdapat pula kelompok pasien yang apabila diberikan kemoterapi tidak memperpanjang survival, namun justru memperpendek.9,10 Dengan penurunan cut point off reseptor estrogen dan progesteron dari 10% menjadi 1% nuclear staining, diperkirakan akan lebih banyak lagi pasien yang mendapatkan terapi hormonal.11 Apabila pemeriksaan genetic microarray digunakan sebagai prediktor maka untuk kasus kanker payudara stadium I dan II yang mengekspresikan reseptor estrogen, kemoterapi hanya berguna pada 25% kasus.10 Data ini juga menunjukkan bahwa terdapat kecenderungan pengurangan penggunaan kemoterapi dan peningkatan jumlah pasien yang hanya diberikan terapi hormonal. …”
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Bioinformatics-driven identification of key non-invasive prognostic biomarkers in hepatocellular carcinoma by Muhammad-Redha Abdullah-Zawawi, Muhammad Irfan Abdul Jalal, Shing Cheng Tan, Loscheni Varma, Nur Alyaa Afifah Md Shahri, Mira Farzana Mohamad Mokhtar, Ryia Illani Mohd Yunos, Nurul Syakima Ab Mutalib, Rahman Jamal

“…Methods Publicly available microarray data within the Gene Expression Omnibus repository were analysed to determine genetic elements that were differentially expressed. …”
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The profiling and analysis of gene expression in rat temporomandibular joint disc tissue and its derived cells by Haruna Kashio, Rie Takai, Ayuko Takada, Yuya Nakao, Nattakarn Hosiriluck, Masahiro Iijima, Yoshihiro Abiko, Itaru Mizoguchi, Toshiya Arakawa

“…Cells derived from the TMJ discs were cultured, and mRNA extracted from the disc tissues and derived cells was analyzed for gene profiling via microarray hybridization. Additionally, we compared the ECM expression between the TMJ disc and the PDL. …”
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miR-21-5p Enriched Exosomes from Human Embryonic Stem Cells Promote Osteogenesis via YAP1 Modulation by Huang X, Zhao Z, Zhan W, Deng M, Wu X, Chen Z, Xie J, Ye W, Zhao M, Chu J

“…Xinqia Huang,1,&ast; Ziquan Zhao,1,&ast; Weiqiang Zhan,1 Mingzhu Deng,1 Xuyang Wu,1 Zhoutao Chen,1 Jiahao Xie,2 Wei Ye,3 Mingyan Zhao,1,4 Jiaqi Chu3,4 1Orthopaedic Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, People’s Republic of China; 2Department of Dermatology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, People’s Republic of China; 3Department of Obstetrics and Gynecology, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, People’s Republic of China; 4Stem Cell Research and Cellular Therapy Center, Affiliated Hospital of Guangdong Medical University, Zhanjiang, Guangdong, 524001, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Jiaqi Chu; Mingyan Zhao, Affiliated Hospital of Guangdong Medical University, No. 57 Renmin Avenue South Road, Zhanjiang, Guangdong, 524001, People’s Republic of China, Email chujiaqi@gdmu.edu.cn; mingyan.zhao@gdmu.edu.cnPurpose: To investigate the osteogenic potential of human embryonic stem cell-derived exosomes (hESC-Exos) and their effects on the differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs).Methods: hESC-Exos were isolated and characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), and Western blotting. hUCMSCs were cultured with hESC-Exos to assess osteogenic differentiation through alizarin red staining, quantitative PCR (qPCR), and Western blotting. miRNA profiling of hESC-Exos was performed using miRNA microarray analysis. In vivo bone regeneration was evaluated using an ovariectomized rat model with bone defects treated with exosome-loaded scaffolds.Results: hESC-Exos significantly promoted the osteogenic differentiation of hUCMSCs, as evidenced by increased alizarin red staining and the upregulation of osteogenesis-related genes and proteins (ALP, RUNX2, OCN). miRNA analysis revealed that miR-21-5p is a key regulator that targets YAP1 and activates the Wnt/β-catenin signaling pathway. …”
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