Analysis of gene expression difference and biological process in chorionic villi of unexplained recurrent spontaneous abortion by Linling Shi, Lun Wei, Meiqiu Lu, Hongmei Ding, Le Bo

“…Abstract Objective To explore the biological relationship between the regulatory signal pathways involved in differentially expressed genes and recurrent spontaneous abortion (RSA) by analyzing the gene expression microarray data of unexplained RSA. Methods The gene expression profile data of chorionic villi from unexplained recurrent abortion with normal karyotype and selective induced abortion were compared. …”
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Expression of apoptotic protease activating factor-1 in adenoid cystic carcinoma of the salivary glands and its clinicopathological relevance by Dožić Branko, Aničić Boban, Sinobad Vladimir, Miković Nikola, Milanović Srđan, Dožić Ivan

“…We used the technique of tissue microarray (TMA blocks). Sections from the TMA mold, 5 μm thick, were stained with the streptavidin-biotin immunohistochemical technique using primary antibodies specific for Apaf-1 (Leica Biosystems, Newcastle, UK). …”
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Characterization of Cellular and Molecular Heterogeneity of Bone Marrow Stromal Cells by Mona Elsafadi, Muthurangan Manikandan, Muhammad Atteya, Jamil Amjad Hashmi, Zafar Iqbal, Abdullah Aldahmash, Musaad Alfayez, Moustapha Kassem, Amer Mahmood

“…Furthermore, gene expression microarray analysis of osteoblast differentiated CL1 versus CL2 showed significant upregulation in CL1 of bone development and osteoblast differentiation genes which included several homeobox genes: TBX15, HOXA2 and HOXA10, and IGF1, FGFR3, BMP6, MCAM, ITGA10, IGFBP5, and ALP. siRNA-based downregulation of the ALP gene in CL1 impaired osteoblastic and adipocytic differentiation. …”
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ANGPTL4 exacerbates pancreatitis by augmenting acinar cell injury through upregulation of C5a by Kyung Hee Jung, Mi Kwon Son, Hong Hua Yan, Zhenghuan Fang, Juyoung Kim, Soo Jung Kim, Jung Hee Park, Ji Eun Lee, Young‐Chan Yoon, Myeong Seong Seo, Beom Seok Han, Soyeon Ko, Young Ju Suh, Joo Han Lim, Don‐Haeng Lee, Ziqiang Teo, Jonathan Wei Kiat Wee, Nguan Soon Tan, Soon‐Sun Hong

“…However, little is known about the genes associated with pancreatitis severity. Our microarray analysis of pancreatic tissues from mild and severe acute pancreatitis mice models identified angiopoietin‐like 4 (ANGPTL4) as one of the most significantly upregulated genes. …”
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Single nucleus RNA-seq reveals the process from onset to chronic kidney disease in IgA nephropathy by Jun-Peng You, Gen-Yang Cheng, Xiao-Xue Zhang, Zheng Wang, Chen Liu, Wei Ning, Ya-Ru Liu, Hui-Miao Hu, Ya-Ling Zhai, Yuan-Yuan Qi, Zhan-Zheng Zhao

“…We analyzed several public transcriptomic datasets, including microarray and bulk RNA-sequencing, to identify pathway alterations in IgAN patients and progressors. …”
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The significance of Trk receptors in pancreatic cancer by Matthew D Johnson, Brandon Stone, Bryan J Thibodeau, Andrew M Baschnagel, Sandra Galoforo, Laura E Fortier, Billie Ketelsen, Samreen Ahmed, Zakiya Kelley, Alaa Hana, Thomas G Wilson, John M Robertson, Robert P Jury, George D Wilson

“…A specific inhibitor of TrkA, B, and C, AstraZeneca 1332, was investigated in vitro and in vivo in combination with radiation. A tissue microarray was constructed from 77 pancreatic ductal adenocarcinoma patients who had undergone neoadjuvant chemoradiation and the Trk receptor, and neurogenic differentiation 1 expression was assessed and correlated with overall survival. …”
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Targeting glial fibrillary acidic protein in glaucoma: a monoclonal antibody approach to modulate glial reactivity and neuroinflammation for neuroprotection by Chaoqiang Guan, Linglin Zhang, Kristian Nzogang Fomo, Jie Yang, Norbert Pfeiffer, Franz H. Grus

“…Retinal ganglion cell (RGC) survival and glial responses were evaluated with immunostaining. Western blot and microarray analyses were performed to investigate molecular and pathway alterations. …”
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Cyclooxygenase-1 deletion in 5 × FAD mice protects against microglia-induced neuroinflammation and mitigates cognitive impairment by Jie Wang, Hong Ni, Yu Wang, Luyao Wei, Hanqing Ding, Zhongzhao Guo, Hao Pan, Ying Yu, Jia Luo, Weidong Pan, Deheng Wang, Zun-Ji Ke

“…In addition, single-cell sequencing and microarray datasets from public databases and ingenuity pathway analysis in vitro were employed to explore gene expression profiles in the brains of AD mice. …”
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Integrated analysis of hub genes and intrinsically disordered regions in triple-negative breast cancer by Azhar Iqbal, Faisal Ali, Sulaiman Ali Alharbi, Muhammad Sajid, Saleh Alfarraj, Momina Hussain, Tehmina Siddique, Rakhshanda Mustaq, Fakhra Shafique, Muhammad Sarfaraz Iqbal

“…Despite several attempts, the molecular pathways of TNBC remain unknown, posing a significant clinical barrier in the search for viable targets. Two microarray datasets were used to identify possible targets for TNBC, GSE38959 and GSE45827, retrieved from the Gene Expression Omnibus (GEO) database. …”
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Transcription factor GATA4 associates with mesenchymal-like gene expression in human hepatoblastoma cells by Tea Soini, Katja Eloranta, Marjut Pihlajoki, Antti Kyrönlahti, Oyediran Akinrinade, Noora Andersson, Jouko Lohi, Mikko P Pakarinen, David B Wilson, Markku Heikinheimo

“…RNA interference was used to inhibit GATA4 in human HUH6 hepatoblastoma cells, and changes in cell migration were measured with wound healing and transwell assays. RNA microarray hybridization was performed on control and GATA4 knockdown HUH6 cells, and differentially expressed genes were validated by quantitative polymerase chain reaction or immunostaining. …”
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Ailanthone disturbs cross-talk between cancer cells and tumor-associated macrophages via HIF1-α/LINC01956/FUS/β-catenin signaling pathway in glioblastoma by Xubin Deng, Qianbing Zhang, Fa Jin, Fengfei Lu, Guosheng Duan, Luwei Han, Meiling Zhu, Zhengyan Yang, Gong Zhang

“…This study aims to explore the biological function and the underlying mechanism of ailanthone in GBM. Methods The microarray analysis was used to screen out down-stream long non-coding RNAs (lncRNAs) targeted by ailanthone. …”
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Screening of endoplasmic reticulum stress characteristic genes and immune infiltration manifestations in chronic obstructive pulmonary disease by ZHANG Shuang, LUO Chenyang, HE Zhiyi

“…Objective To identify the core genes of endoplasmic reticulum stress (ERS) in chronic obstructive pulmonary disease (COPD) using the bioinformatics, various machine learning algorithms and experimental validation. Methods The microarray data GSE5058, GSE8545, and GSE19407 were downloaded from the GEO database to identify differentially expressed genes (DEGs) between airway epithelial cells of COPD smokers and non-smokers, and then the common DEGs were obtained after overlapping with ERS-related genes and enriched for analysis. …”
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WDR3 undergoes phase separation to mediate the therapeutic mechanism of Nilotinib against osteosarcoma by Minglei Li, Nan Li, Yuying Fan, Zhan Zhang, Long Zhou, Yifan Yu, Man Ni, Mingzi Tan, WanJie Huang, Tong Zhu

“…Therefore, mining prognostic markers related to LLPS could allow patients to benefit from targeted therapies. Method Microarray analysis was performed to identify LLPS-related biomarkers, followed by the validation of binding interactions between genes and drugs via molecular docking analysis. …”
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FABP4 suppresses proliferation and invasion of hepatocellular carcinoma cells and predicts a poor prognosis for hepatocellular carcinoma by Cheng‐Qian Zhong, Xiu‐Ping Zhang, Ning Ma, Er‐Bin Zhang, Jing‐Jing Li, Ya‐Bo Jiang, Yu‐Zhen Gao, Yan‐Mei Yuan, Shi‐Qian Lan, Dong Xie, Shu‐Qun Cheng

“…In our study, we investigated the expression of FABP4 at both mRNA and protein levels, and by examining 175 cases of patients with cancer of the liver tissue microarray, the significance between the expression of FABP4 and clinical characteristics had been discussed. …”
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Identification of circulating microRNAs as potential biomarkers for hepatic necroinflammation in patients with autoimmune hepatitis by Koji Fujita, Juntaro Matsuzaki, Satoko Takizawa, Takahiro Ochiya, Masayuki Saruta, Yasuni Nakanuma, Chisato Saeki, Masahiko Kuroda, Makiko Ichikawa, Keiko Takano, Tsunekazu Oikawa, Akihito Tsubota

“…This study aimed to identify highly expressed circulating miRNAs in patients with autoimmune hepatitis (AIH) and to evaluate their association with clinical characteristics.Methods Microarray analyses were performed, and miRNA expression profiling for AIH, primary biliary cholangitis (PBC) and overlap syndrome (OS) using the serum of patients and healthy individuals was done. …”
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Identification of <i>hsa_circ_0018905</i> as a New Potential Biomarker for Multiple Sclerosis by Valeria Lodde, Ignazio Roberto Zarbo, Gabriele Farina, Aurora Masia, Paolo Solla, Ilaria Campesi, Giuseppe Delogu, Maria Rosaria Muroni, Dimitrios Tsitsipatis, Myriam Gorospe, Matteo Floris, Maria Laura Idda

“…Here, we explore the panorama of circRNA expression in PBMCs purified from five newly diagnosed MS patients and five healthy controls (HCs) using the Arraystar Human circRNAs microarray. Experimental validation was then carried out in a validation cohort, and a possible correlation with disease severity was tested. …”
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CD68 as a multi-omic prognostic biomarker in digestive system cancers: correlations with tumor-infiltrating immune cells and immune checkpoints by Hui Li, Hui Zhang, Rujiang Dai, Dian Zheng, Jianghai Zhao, Hong Jing, Xuhui Ma, Lin Zhang, Weihong Sun, Zhimin Suo

“…This study aims to investigate the expression levels, prognostic significance, and clinical implications of CD68, as well as its correlations with six TIICs and four common TICs in DSC.Materials and methodsWe analyzed CD68 mRNA and protein expression using online databases and immunohistochemistry (IHC) on tissue microarray (TMA) sections, comparing DSC tumor tissues with adjacent normal tissues. …”
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Comprehensive bioinformatics analysis identifies hub genes associated with immune cell infiltration in early-onset schizophrenia by Shasha Wu, Tailian Xue, Yilin Li, Weikang Chen, Yan Ren

“…Methods In this study, we recruited 26 EOS patients and 27 healthy controls (HCs), and microarray data were collected. Crossover genes were identified using weighted gene co-expression network analysis (WGCNA) and differential expression genes (DEGs) analysis. …”
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Identification of CD36 as a contributor in inflammatory response of rheumatoid arthritis and screening of feasible bioactive drugs targeting it by Dan Xuan, Xiaowan Wang, Dandan Feng, Li Wang, Yonghui Xia

“…Methods RA-related microarray datasets (GSE77298 and GSE206848) and inflammatory genes (IRGs) were downloaded from Gene Expression Omnibus database and GeneCards database, respectively. …”
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Identification and validation of diagnostic genes IFI44 and IRF9 in insomnia-associated autoimmune uveitis by Chao Wu, Hui Feng, Meng Tian, Baorui Chu, Xianyang Liu, Shuhao Zeng, Yakun Wang, Hong Wang, Shengping Hou, Qingfeng Liang

“…We investigated insomnia-associated genes that may contribute to AU pathogenesis and sought to identify potential biomarkers for insomnia-associated AU.MethodsMicroarray data related to insomnia and AU were downloaded from the Gene Expression Omnibus (GEO) database and analyzed. …”
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