Poor clinical outcomes and immunoevasive contexture in CD161+CD8+ T cells barren human pancreatic cancer by Jun Yu, Liang Liu, Hanlin Yin, Qiangda Chen, Siyao Liu, Wenchuan Wu, Ning Pu, Wenhui Lou, Zhenlai Jiang, Taochen He, Yuqi Xie, Weilin Mao, Jiande Han, Joseph R Habib

“…CD161+CD8+ T cell infiltration was assessed using immunofluorescence staining on tumor microarrays. Flow cytometry and single-cell RNA sequencing were used to evaluate their functional status.Results We observed significant associations between tumor-infiltrating CD161+CD8+ T cells and clinicopathological factors, such as tumor differentiation, perineural invasion, and serum CA19-9 levels. …”
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Loss of OBSCN expression promotes bladder cancer progression but enhances the efficacy of PD-L1 inhibitors by Tao Wang, Tuanjie Guo, Juanjuan Sun, Xinyue Zang, Lei Dong, Jian Zhang, Siteng Chen, Guihua Chen, Sicong Ma, Xinyu Zhai, Chuanmin Chu, Chaofu Wang, Xiang Wang, Dongliang Xu, Mingyue Tan

“…Immunohistochemistry (IHC) was performed on tissue microarrays including 80 BLCA patients from Shuguang Hospital. …”
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HERV-derived epitopes represent new targets for T-cell-based immunotherapies in ovarian cancer by Qing Wang, Maha Ayyoub, Olivia Le Saux, Paola Bonaventura, Isabelle Treilleux, Nicolas Chopin, Clarisse Dubois, Virginie Mutez, Nicolas Chuvin, Yann Estornes, Jenny Valladeau-Guilemond, Stephane Depil, Nicolas Gadot, Marie Michelas, Audrey Page, Olivier Tabone, Marie Delles, Sarah Moran, Thibault Richard, Marjorie Lacourrège, Dina M Tawfik, Ema Etchegaray, Adrian Valente, Rasha E Boulos, Gabriel Jimenez Dominguez

“…The presence of HERV-K/HML-2 Gag antigen was then assessed by immunohistochemistry (IHC) on tumor microarrays from ovarian cancer samples and normal ovarian tissues. …”
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Unique characteristics of tertiary lymphoid structures in kidney clear cell carcinoma: prognostic outcome and comparison with bladder cancer by Tatsuhiko Tsunoda, Kazuhiro Kakimi, Shuji Mikami, Mototsugu Oya, Tsukasa Masuda, Nobuyuki Tanaka, Kimiharu Takamatsu, Kyohei Hakozaki, Ryohei Takahashi, Tadatsugu Anno, Ryohei Kufukihara, Kazunori Shojo, Toshiaki Shinojima, Eriko Aimono, Hiroshi Nishihara, Ryuichi Mizuno

“…Background The aims of this study were (1) to clarify the impact of tertiary lymphoid structure (TLS) status on the outcome and immunogenomic profile of human clear cell renal cell carcinoma (ccRCC) and (2) to determine phenotypic differences in TLSs between different types of genitourinary cancer, that is, urinary ccRCC and bladder cancer.Methods We performed a quantitative immunohistological analysis of ccRCC tissue microarrays and conducted integrated genome mutation analysis by next-generation sequencing and methylation array analysis. …”
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The LysoPS/GPR174 axis drives metastatic progression in esophageal squamous cell carcinoma through cAMP-PKA-CREB signaling activation by Rong Xiao, Pei Xu, Xiangyuan Li, Feng Shen, Shuangfen Tao, Xiaocen Zhu, Yu Cai, Zhuowei Feng, Zhiyi Liu, Haibo Xiao, Fangbao Ding, Meiling Zhu

“…The correlation between GPR174 expression and ESCC prognosis was analyzed using ESCC tissue microarrays, immunohistochemistry, and Kaplan—Meier survival analysis. …”
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Quantitative and Qualitative Variations in TILs in Risk Stratification and Patient Selection for Neoadjuvant Chemotherapy of the Luminal B and Triple-Negative Breast Cancer Immunop... by Ana Car Peterko, Koraljka Rajković Molek, Anita Savić Vuković, Tamara Gulić, Petra Valković Zujić, Eleonora Cini Tešar, Damir Juranić, Aleksandra Pirjavec Mahić, Franjo Lovasić, Gordana Đorđević, Manuela Avirović

“…Quantitative and qualitative evaluations of TILs (Tumor-Infiltrating Lymphocytes) were performed on tissue microarrays constructed from pretreatment core-needle biopsy tumor specimens. …”
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Engineered EVs from LncEEF1G - overexpressing MSCs promote fibrotic liver regeneration by upregulating HGF release from hepatic stellate cells by Jiebin Zhang, Xiaotong Qiu, Yunguo Lei, Haitian Chen, Dongwei Wu, Tingting Wang, Xin Sui, Jiaqi Xiao, Chenhao Jiang, Huayao Zhang, Yasong Liu, Xiaoquan Liu, Yingcai Zhang, Xu Che, Ye Lin, Jia Yao, Zihao Pan, Rong Li, Jun Zheng

“…However, there is still no clinically effective treatment for promoting fibrotic liver regeneration because the underlying hepatocellular mechanism remains poorly understood. Through microRNA microarrays combined with the application of AAV6, we found that high expression of miR-181a-5p in activated hepatic stellate cells (HSCs) suppressed the expression of hepatic growth factor (HGF) and partially contributed to impaired regeneration potential in mice with hepatic fibrosis that had undergone two-thirds partial hepatectomy. …”
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Integrated hepatic transcriptomics and metabolomics identify Pck1 as a key factor in the broad dysregulation induced by vehicle pollutants by Gajalakshmi Ramanathan, Yuqi Zhao, Rajat Gupta, Siri Langmo, May Bhetraratana, Fen Yin, Will Driscoll, Jerry Ricks, Allen Louie, James A. Stewart, Timothy R. Gould, Timothy V. Larson, Joel Kaufman, Michael E. Rosenfeld, Xia Yang, Jesus A. Araujo

“…Methods Apolipoprotein E knockout (ApoE KO) mice were exposed to DE or filtered air (FA) for two weeks, or DE for two weeks followed by FA for 1 week. Expression microarrays and global metabolomics assessment were performed in the liver. …”
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RET podría actuar como un gen supresor de tumores en células de cáncer de cuello uterino by Mariana A. Martínez-Castillo, María A. Hernández-Cueto, Keiko Taniguchi-Ponciano, Mauricio Salcedo

“…Material y métodos: Se evaluaron mediante inmunohistoquímica microarrays de tejido sano, de lesiones intraepiteliales escamosas de bajo y alto grado, y de tejidos de CC. …”
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Identification of a Serum Exosome-Derived lncRNA‒miRNA‒mRNA ceRNA Network in Patients with Endometriosis by Yan Huang, Deyu Zhang, Yingfang Zhou, Chao Peng

“…Exosomal miRNA was sequenced using microarrays. EM-related differential miRNAs (DE-miRNAs) were identified using analysis of differential miRNA expression and weighted coexpression network analysis. …”
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The biomarker potential of circPOLD1 and its binding protein YBX1 in cervical carcinogenesis by Lu Zhao, Xin Chen, Yanan Zhang, Yixuan Cen, Tingjia Zhu, Lingfang Wang, Lili Xia, Yang Li, Xiaodong Cheng, Xing Xie, Weiguo Lu, Junfen Xu

“…BaseScope and immunohistochemistry (IHC) were applied to tissue microarrays for clincial validation and ROC curve analysis evaluated the diagnostic performance of circPOLD1 in serum as a liquid biopsy marker. …”
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Kinomic profiling to predict sunitinib response of patients with metastasized clear cell Renal Cell Carcinoma by Jeannette C. Oosterwijk-Wakka, Liesbeth Houkes, Loes F.M. van der Zanden, Lambertus A.L.M. Kiemeney, Kerstin Junker, Anne Y Warren, Tim Eisen, Ulrich Jaehde, Marius T Radu, Rob Ruijtenbeek, Egbert Oosterwijk

“…Phosphotyrosine-activity profiling was performed using PamChip® peptide microarrays (144 peptides derived from known phosphorylation sites in Protein Tyrosine Kinase substrates) of lysed tissue samples (5 µg protein input) of 163 mRCC patients. …”
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Potential common mechanisms between primary Sjögren’s syndrome and Hashimoto’s thyroiditis: a public databases-based study by Yanjun Lin, Shupin Tang, Yan Lin, Rihui Wang, Yifeng Xing, Zonghe Xu, Yan Li, Qingxia Fang, Wenwei Wei, Dong Wu, Jiang Chen

“…This study aims to explore the underlying mechanism.MethodsWe screened differentially expressed genes (DEGs) in the pSS and HT-related transcriptomic microarrays. Based on KEGG, PID, Reactome, and BioCarta enrichment analysis, pathway annotations were performed. …”
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RETRACTED: The long noncoding RNA AK002107 negatively modulates miR‐140‐5p and targets TGFBR1 to induce epithelial–mesenchymal transition in hepatocellular carcinoma by Yun‐Hua Tang, Guo‐Lin He, Shan‐Zhou Huang, Ke‐Bo Zhong, Hui Liao, Lei Cai, Yi Gao, Zhen‐Wei Peng, Shun‐Jun Fu

“…The differential expression of lncRNAs between HCC and paired nontumor tissues was identified using microarrays, and the correlation between the expression of lncRNA AK002107 and the clinical prognosis of HCC was analyzed. …”
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Rapid Detection of Panton–Valentine Leukocidin Production in Clinical Isolates of <i>Staphylococcus aureus</i> from Saxony and Brandenburg and Their Molecular Characterisation by Elke Müller, Stefan Monecke, Marc Armengol Porta, Marco Vinicio Narvaez Encalada, Annett Reissig, Lukas Rüttiger, Percy Schröttner, Ilona Schwede, Hans-Herman Söffing, Alexander Thürmer, Ralf Ehricht

“…They were also characterized using DNA microarrays, facilitating the detection of virulence and resistance markers as well as the assignment to clonal complexes and epidemic/pandemic strains. …”
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Effector T cells under hypoxia have an altered transcriptome similar to tumor-stressed T cells found in non-responsive melanoma patients by Ahmad A Tarhini, Zhihua Chen, Dongliang Du, Joshua Davis, Justin C Boucher, Katarzyna A Rejniak, Jose A Guevara-Patino, Timothy I Shaw, Alyssa Obermayer, Mate Z Nagy, Lourdes B Plaza-Rojas, Elena Kostenko, Anna L Austin, Awino Maureiq E Ojwang'

“…As T cell-based therapies often fail to work in solid tumors, this study aims to investigate the effects of hypoxia on T cell topo-distribution in the TME, gene expression association with T cell states, and clinical responses in melanoma.Methods To generate detailed information on tumor oxygenation and T cell accessibility, we used mathematical modeling of human melanoma tissue microarrays that incorporate oxygen supply from vessels, intratumoral diffusion, and cellular uptake. …”
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Inflammatory markers after supplementation with marine n-3 or plant n-6 PUFAs: A randomized double-blind crossover study by Elise Grytten, Johnny Laupsa-Borge, Kaya Cetin, Pavol Bohov, Jan Erik Nordrehaug, Jon Skorve, Rolf K. Berge, Elin Strand, Bodil Bjørndal, Ottar K. Nygård, Espen Rostrup, Gunnar Mellgren, Simon N. Dankel

“…Cytokines and chemokines (multiplex assay), acute-phase proteins (MALDI-TOF mass spectrometry), endothelial function (vascular reaction index), blood pressure, FA composition (red blood cell membranes/serum/adipose tissue, GC-MS/MS), and adipose gene expression (microarrays, quantitative PCR) were measured. While significant differences between treatments in relative change scores were found for systolic blood pressure (n-3 vs. n-6: −1.81% vs. 2.61%, P = 0.003), no differences between n-3 and n-6 were found for any circulatory inflammatory markers. …”
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The alteration of IL-17 signaling pathway in bipolar disorder: a preliminary study with transcriptomic perspective by Xiaobo Wang, Xiaobo Wang, Su Yu, Zhen Gao, Fangming Xu, Yumei Wang, Yumei Wang, Yumei Wang, Tianle Zhang, Tingting Xie, Xihua Jia

“…This study aims to explore global mRNA expression in Peripheral blood white cells (PBMC) of BD patients.MethodsWe analyzed whole-genome gene expression profiles from PBMC of 12 BD states-manic (BD-M), 12 BD states-depressive (BD-D), and 12 matched healthy controls (HC) using microarrays. Validation of differentially expressed genes within the IL-17 pathway was conducted through qRT-PCR, while ELISA measured specific protein levels. …”
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Multiplex imaging reveals novel patterns of MRTFA/B activation in the breast cancer microenvironment by Stephanie M. Wilk, Kihak Lee, Caitlyn C. Castillo, Mohamed Haloul, Alexa M. Gajda, Virgilia Macias, Elizabeth L. Wiley, Zhengjia Chen, Xinyi Liu, Xiaowei Wang, Maria Sverdlov, Kent F. Hoskins, Ekrem Emrah Er

“…Methods We used a multiplexed immunofluorescence approach to label MRTFA, MRTFB, tumor cells by using pan Cytokeratin, endothelial cells by using CD31, and antigen presenting cells (APCs) by using HLA-DRA on two different breast cancer tissue microarrays (TMA): The breast cancer progression TMA provided by the Cooperative Human Tissue Network (CHTN_BrCaProg3) and the University of Illinois Breast Cancer Working Group (TMA BCWG UIC-001-TMA) that included primary tumor and lymph node metastases from patients residing in the West Side and South Side of Chicago. …”
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Ultracompact On-Chip Multiplexed Sensor Array Based on Dense Integration of Flexible 1-D Photonic Crystal Nanobeam Cavity With Large Free Spectral Range and High Q-Factor by Daquan Yang, Bo Wang, Xin Chen, Chuan Wang, Yuefeng Ji

“…With proper engineering of the FSR, multiple ultracompact high-sensitivity 1-D-PCNC sensors can be integrated into microarrays without resonance overlap, and be interrogated simultaneously between a single input/output ports. …”
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