Transcriptomic analysis of neural stem and progenitor cells in comparison with glioblastoma stem cells by V. E. Shevchenko, N. E.  Arnotskaya, T.  I. Kushnir, A. S. Bryukhovetskiy

“…Used: highly sensitive transcriptomic analysis on high-density microarrays, cellular technologies, modern bioinformatics analysis.Results. …”
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Cost-effective solutions for high-throughput enzymatic DNA methylation sequencing. by Amy Longtin, Marina M Watowich, Baptiste Sadoughi, Rachel M Petersen, Sarah F Brosnan, Kenneth Buetow, Qiuyin Cai, Cayo Biobank Research Unit, Michael D Gurven, James P Higham, Heather M Highland, Yi-Ting Huang, Hillard Kaplan, Thomas S Kraft, Yvonne A L Lim, Jirong Long, Amanda D Melin, Michael J Montague, Jamie Roberson, Kee Seong Ng, Michael L Platt, India A Schneider-Crease, Jonathan Stieglitz, Benjamin C Trumble, Vivek V Venkataraman, Ian J Wallace, Jie Wu, Noah Snyder-Mackler, Angela Jones, Alexander G Bick, Amanda J Lea

“…., assays that rely on microarrays, enzyme digests, or sequence capture to target a subset of the genome). …”
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Lactobacilli-host interactions inhibit Staphylococcus aureus and Escherichia coli-induced cell death and invasion in a cellular model of infection by Despoina Eugenia Kiousi, Maria Panopoulou, Aglaia Pappa, Alex Galanis

“…Dual RNA-seq and protein microarrays were used to determine expression changes in L125 and host cells during co-incubation. …”
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Cellular expression, trafficking, and function of two isoforms of human ULBP5/RAET1G. by Robert A Eagle, Gillian Flack, Anthony Warford, Jesús Martínez-Borra, Insiya Jafferji, James A Traherne, Maki Ohashi, Louise H Boyle, Alexander D Barrow, Sophie Caillat-Zucman, Neil T Young, John Trowsdale

“…Using specific anti-RAET1G1 antiserum to stain tissue microarrays we show that RAET1G1 expression is highly restricted in normal tissues. …”
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Adult B-Cell Acute Lymphoblastic Leukaemia Antigens and Enriched Pathways Identify New Targets for Therapy by Eithar Mohamed, Sara Goodman, Leah Cooksey, Daniel M. Fletcher, Olivia Dean, Viktoriya B. Boncheva, Ken I. Mills, Kim H. Orchard, Barbara-ann Guinn

“…Methods: aB-ALL antigens were identified using different sources—immunoscreening, protoarrays, two microarrays and one cancer-testis antigen database, and a review of the genomic analyses of aB-ALL. …”
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Impact of HIF-1α, LOX and ITGA5 Synergistic Interaction in the Tumor Microenvironment on Colorectal Cancer Prognosis by Hayriye Tatlı Doğan, Mehmet Doğan, Seda Kahraman, Doğukan Çanakçı, Mehmet Ali Nahit Şendur, Mustafa Tahtacı, Fazlı Erdoğan

“…<b>Methods</b>: HIF-1α, LOX and ITGA5 antibodies were applied immunohistochemically to tissue microarrays prepared from tumor samples. Expression status in the tumor microenvironment were evaluated using a combined scoring system based on staining intensity and the percentage of positively stained cells. …”
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Expression of Cancer‐Testis Antigens MAGE‐A1, MAGE‐A4, NY‐ESO‐1 and PRAME in Bone and Soft Tissue Sarcomas: The Experience From a Single Center in China by Anni Chen, Yuling Qiu, Ying‐Tzu Yen, Chun Wang, Xiaolu Wang, Chunhua Li, Zijian Wei, Lin Li, Lixia Yu, Fangcen Liu, Rutian Li

“…Methods and Results We employed immunohistochemistry and multiplex immunostaining microarrays (MI chips) to assess the expression of MAGE‐A4, PRAME, MAGE‐A1, KK‐LC‐1, and NY‐ESO‐1 in 21 cases of undifferentiated pleomorphic sarcoma (UPS), 26 cases of smooth muscle sarcoma, 28 cases of liposarcoma, 40 cases of osteosarcoma (OS), and 13 cases of chondrosarcoma. …”
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Pre-diagnostic Demographic, Lifestyle, and Health History Factors in Association with Secreted Protein Acidic and Rich in Cysteine (SPARC) Expression in Colorectal Cancer Tissue by Umaimah Zanif, Jaclyn Parks, Isabella Tai, Stephen Yip, Sindy Babinszky, Katy Milne, Peter Watson, Rachel A. Murphy, Parveen Bhatti

“…Methods: Tumor and normal tissue cores were taken from formalin-fixed paraffin-embedded (FFPE) blocks of incident CRC cases and were used to create tissue microarrays (TMAs). Slides created from the TMAs were stained with SPARC antibodies and analyzed to calculate H-scores for both epithelial and non-epithelial components of tumor and normal tissues. …”
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Schlafen 11 Expression in Patients With Small Cell Lung Cancer and Its Association With Clinical Outcomes by Ken Masuda, Tatsuya Yoshida, Noriko Motoi, Yuki Shinno, Yuji Matsumoto, Yusuke Okuma, Yasushi Goto, Hidehito Horinouchi, Noboru Yamamoto, Shun‐ichi Watanabe, Tomoaki Hoshino, Yasushi Yatabe, Yuichiro Ohe

“…SLFN‐11 expression was assessed using immunohistochemistry in tissue microarrays and scored using a histology (H)‐score (range: 0–300). …”
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MARCKSL1 interacted with F‐actin to promote esophageal squamous cell carcinoma mobility by modulating the formation of invadopodia by Yue Zhao, Xiufeng Xie, Lusong Tian, Fang Liu, Yulin Sun, Haizhen Lu, Xiaohang Zhao, Yousheng Mao

“…Finally, the correlation between MARCKSL1 and the clinicopathological features of ESCC patients was assessed based on TCGA database analysis and immunohistochemistry staining of tissue microarrays. Results Knockdown of MARCKSL1 markedly attenuated the cell motility capacity of ESCC cells in vitro, while MARCKSL1 overexpression had the opposite effect. …”
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Low density of intratumoral M1-macrophage infiltration may correlate with worse prognosis in low-grade early-stage uterine endometrioid carcinoma by Michiko Nagamine, Hiroshi Ogi, Maki Hirai, Ikoi Omatsu, Sanzo Moriwaki, Saya Shibata, Satoru Yasukawa, Kaori Yoriki, Motohiro Kojima, Taisuke Mori, Kyoko Itoh, Eiichi Konishi

“…Multiplex immunohistochemistry was performed on tissue microarrays constructed from formalin-fixed paraffin-embedded tissue blocks of primary surgical specimens and, when available, recurrent lesions. …”
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Cancer-associated fibroblast driven paracrine IL-6/STAT3 signaling promotes migration and dissemination in invasive lobular carcinoma by Esme Bullock, Aleksandra Rozyczko, Sana Shabbir, Ifigenia Tsoupi, Adelaide I.J. Young, Jana Travnickova, Laura Gómez-Cuadrado, Zeanap Mabruk, Giovana Carrasco, Elizabeth Morrow, Kathryn Pennel, Pim Kloosterman, Julia M Houthuijzen, Jos Jonkers, Lidia Avalle, Valeria Poli, Richard Iggo, Xue Xiao, Jingjing Guo, Xuan Zhu, Elizabeth Mallon, Joanne Edwards, E. Elizabeth Patton, Valerie G. Brunton

“…Bioinformatic analysis of public databases and immunohistochemical of human tumor microarrays was carried out. Results We identified IL-6 as a paracrine CAF-derived factor that activates Signal-Transducer-and-Activator-of-Transcription-3 (STAT3) in human and mouse ILC models. …”
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LncRNA MBNL1‐AS1 represses cell proliferation and enhances cell apoptosis via targeting miR‐135a‐5p/PHLPP2/FOXO1 axis in bladder cancer by Xiaosong Wei, Xiaoming Yang, Beibei Wang, Yang Yang, Zhiwei Fang, Chengzhi Yi, Lei Shi, Dongkui Song

“…Abstract LncRNAs have been shown to play essential roles in bladder cancer (BC) progress. Our microarrays of clinical samples firstly screened that lncRNA muscleblind‐like 1 antisense RNA 1 (MBNL1‐AS1) was poorly expressed in BC tissues. …”
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ASF1B promotes gastric cancer progression by modulating H2AC20 and activating PI3K/AKT and ERK1/2 pathways by Mengyuan Zhao, Junchang Zhang, Yanjun He, Chongge You

“…With the continuous enrichment of treatment methods, GC has entered an era of comprehensive treatment oriented toward precision and standardization.MethodsThrough the application of bioinformatics and assessments of tissue microarrays, this study has selected the histone chaperone Anti-Silencing Function 1B (ASF1B) for detailed analysis, including clinical specimens. …”
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STING agonist-based treatment promotes vascular normalization and tertiary lymphoid structure formation in the therapeutic melanoma microenvironment by Walter J Storkus, Ronald J Fecek, Manoj Chelvanambi, Jennifer L Taylor

“…In vitro assays were performed on CD11c+ BMDCs treated with 2.5 µg/mL ADU S-100 or CD11c+ DCs isolated from tumor digests and associated transcriptional changes analyzed via qPCR or profiled using DNA microarrays. For T cell repertoireβ-CDR3 analyses, T cell CDR3 was sequenced from gDNA isolated from splenocytes and enzymatically digested tumors.Results We report that activation of STING within the TME leads to slowed melanoma growth in association with increased production of antiangiogenic factors including Tnfsf15 (Vegi) and Cxcl10, and TLS-inducing factors including Ccl19, Ccl21, Lta, Ltb and Light. …”
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In vitro methodologies to evaluate nanocarriers for cancer treatment: where are we? by Giovanna Cassone Salata, Camila Megumi Hirokawa, Giovanna Barros de Melo, Isabella Draszesski Malagó, Jessica Ribeiro Nunes, Julia Sapienza Passos, Regina Gomes Daré, Luciana B. Lopes

“…., apoptosis, necrosis, and autophagy), signaling pathways exploring gene expression analysis (qRT-PCR, RNA-seq, microarrays) and protein expression analysis (western blot, immunochemistry, mass spectrometry), oxidative stress evaluation, and cell migration/invasion assays (e.g., scratch/wound-healing, transwell, and microfluidic chip-based models). …”
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Clinical value of miR-145-5p in NSCLC and potential molecular mechanism exploration: A retrospective study based on GEO, qRT-PCR, and TCGA data by Ting-qing Gan, Zu-cheng Xie, Rui-xue Tang, Tong-tong Zhang, Dong-yao Li, Zu-yun Li, Gang Chen

“…First, a meta-analysis was performed with eligible literature, followed by microRNA microarrays in the Gene Expression Omnibus database, to verify the diagnostic and prognostic values of miR-145-5p. …”
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Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer. by Jie Chen, Ana R Gomes, Lara J Monteiro, San Yu Wong, Lai Han Wu, Ting-Ting Ng, Christina T Karadedou, Julie Millour, Ying-Chi Ip, Yuen Nei Cheung, Andrew Sunters, Kelvin Y K Chan, Eric W-F Lam, Ui-Soon Khoo

“…<h4>Methodology/principal findings</h4>Examination of FOXO3a and phosphorylated-Akt (P-Akt) expression in breast cancer tissue microarrays showed nuclear FOXO3a was associated with lymph node positivity (p = 0.052), poor prognosis (p = 0.014), and P-Akt expression in invasive ductal carcinoma. …”
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Survivin Expression in Placentas with Intrauterine Growth Restriction by Pavo Perković, Sanja Štifter-Vretenar, Marina Perković, Marko Štefančić, Ena Holjević, Andrea Dekanić, Tea Štimac

“…<b>Methods</b>: Survivin presence was determined in 153 archival formalin-fixed and paraffin-embedded placental tissues from IUGR (N = 122) and uncomplicated (N = 31) term pregnancies. Tissue microarrays (TMAs) were constructed, and survivin expression was assessed using immunohistochemistry (IHC). …”
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GPX3 promotes cisplatin resistance in TNBC by manipulating ROS-TGFB1-ZEB2 by Qingyi Hu, Qianzhi Chen, Wen Yang, Anwen Ren, Jie Tan, Tao Huang

“…The correlation between the expression level of GPX3, TGFB1, and ZEB2 was analyzed using breast cancer microarrays and the TCGA database. The effect of GPX3 on platinum sensitivity in TNBC was studied using a mouse xenograft model. …”
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