Pharmacological inhibition of carbonic anhydrases with a positively charged pyridinium sulfonamide phenocopies the neuroprotective effects of Car9 genetic ablation in a murine sett... by Sara Amiranda, Mariangela Succoio, Serenella Anzilotti, Ornella Cuomo, Tiziana Petrozziello, Valentina Tedeschi, Arianna Finizio, Giorgia Mele, Seppo Parkkila, Lucio Annunziato, Giuseppina De Simone, Giuseppe Pignataro, Agnese Secondo, Nicola Zambrano

“…The treatments exerted neuroprotective effects on neurons from Car9 knockout mice. Moreover, administration of the sulfonamide inhibitor to rats subjected to transient middle cerebral artery occlusion decreased infarct volumes and improved neurological deficits. …”
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Immunogenic dying cells elicit potent anti-tumor T cell immunity against lung metastasis and tumorigenesis by Min Hu, Xinyu Meng, Tong Wang, Yifan Wang, Xiaodong Chen, Dongliang Xu, Wei He, Hongjia Zhang, Wenzheng Guo, Bo Jing, Siwei Zhang, Jianhua Xu, Beibei Sun, Xueqian Sun, Tingting Liu, Na Ni, Tongtong Zhang, Wenwen Cui, Xiaoyu Wu, Liping Xia, Feng Yao, Fang Zhang, Jing Du, Jiong Deng

“…We immunized mice with ICD dying tumor cells in prevention, therapy in lung metastasis models, and Gprc5a-knockout (ko) model of lung tumorigenesis. T cells and macrophages isolated from lymph nodes or tumor tissues were analyzed by flow cytometry. …”
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Acetyl-CoA synthesis in the skin is a key determinant of systemic lipid homeostasis by Phuong T.T. Nguyen, Mia Shiue, Nina Kuprasertkul, Pedro Costa-Pinheiro, Luke T. Izzo, Laura V. Pinheiro, Hayley A. Affronti, Gabriel Gugiu, Shivani Ghaisas, Joyce Y. Liu, Jordan C. Harris, Charles W. Bradley, John T. Seykora, Xiaolu Yang, Taku Kambayashi, Clementina Mesaros, Brian C. Capell, Kathryn E. Wellen

“…Here, we developed inducible whole-body Acly-knockout mice to determine the requirement for ACLY in normal tissue functions, uncovering its crucial role in skin homeostasis. …”
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JAK/STAT3 signaling promotes pain and depression-like behaviors in rats with bone cancer pain by regulating Th17 cell differentiation by Shuyan Wu, Jundan Jiang, Danfeng Wang, Daoyi Lin, Mingxue Lin, Pinzhong Chen, Jianghu Chen, Honghong Zhang, Ying Wang, Xiaohui Chen, Xiaochun Zheng

“…Rats experiencing RORγt knockout in the amygdala showed reduced microglial activation levels. …”
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Multi-omics unveils strain-specific neuroactive metabolite production linked to inflammation modulation by Bacteroides and their extracellular vesicles by Basit Yousuf, Walid Mottawea, Galal Ali Esmail, Nazila Nazemof, Nour Elhouda Bouhlel, Emmanuel Njoku, Yingxi Li, Xu Zhang, Zoran Minic, Riadh Hammami

“…We used CRISPR/Cas12a-based gene engineering to create a knockout mutant lacking the glutamate decarboxylase gene (gadB) to demonstrate the specific contribution of Bacteroides finegoldii-derived GABA in modulating intestinal homeostasis. …”
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Mitochondrial reactive oxygen species trigger metformin-dependent antitumor immunity via activation of Nrf2/mTORC1/p62 axis in tumor-infiltrating CD8T lymphocytes by Kazuhiro Kakimi, Hirokazu Matsushita, Masaaki Komatsu, Eiryo Kawakami, Mikako Nishida, Nahoko Yamashita, Taisaku Ogawa, Keita Koseki, Eiji Warabi, Tomoyuki Ohue, Katsuyuki Shiroguchi, Heiichiro Udono

“…In addition, conditional knockout mice for Nrf2 and p62 were used to detect these markers, together with the monitoring of in vivo tumor growth. …”
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Reduction of intestinal RIPK1 ameliorates HFD-induced metabolic disorders in female mice by Ye Yuan, Xiaomin Hu, Chunguang Guo, Yihua Xu, Shihan Li, Wei Wen, Xinli Hu, Fanxin Zeng, Weiyi Cui, Wenli Chen, Xueting Sun, Ning Hou, Jue Wang, Rui-Ping Xiao, Xiuqin Zhang

“…In this study, using receptor-interacting protein kinase 1 (Ripk1) intestine-specific heterozygous knockout mice (Ripk1IEC+/−) and high-fat diet (HFD)-feeding mouse model, we report that HFD-induced shift in the transcriptional profile of the ileum toward that of the jejunum, characterized by increased expression of jejunal feature genes in the ileum, are attenuated in Ripk1IEC+/− female mice, but not in males. …”
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Safety and efficacy of edoxaban monotherapy after bioabsorbable polymer everolimus-eluting stent implantation in a human-like coronary atherosclerotic porcine model by Daisuke Kitano, Suguru Migita, Yuxin Li, Yutaka Koyama, Katsunori Fukumoto, Sayaka Shimodai-Yamada, Akira Onishi, Daiichiro Fuchimoto, Shunichi Suzuki, Yoshiyuki Nakamura, Atsushi Hirayama, Hiroyuki Hao, Yasuo Okumura

“…The objective of this study was to evaluate the safety and efficacy of DOAC monotherapy, specifically using factor Xa inhibitors such as edoxaban, in a low-density lipoprotein receptor knockout (LDL-R−/−) miniature pig model of human-like unstable coronary plaques compared to conventional dual-antiplatelet therapy (DAPT). …”
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HINT1 suppression protects against age-related cardiac dysfunction by enhancing mitochondrial biogenesis by Michio Sato, Tsuyoshi Kadomatsu, Jun Morinaga, Yuya Kinoshita, Daisuke Torigoe, Haruki Horiguchi, Sumio Ohtsuki, Shuji Yamamura, Ryoko Kusaba, Takanori Yamaguchi, Goro Yoshioka, Kimi Araki, Tomohiko Wakayama, Keishi Miyata, Koichi Node, Yuichi Oike

“…In addition, cardiac aging phenotypes and mitochondrial biogenesis were also analyzed in 25-month-old cardiac-specific Hint1 knockout mice, 24-month-old CAG-Caren Tg mice, and 24-month-old wild-type mice injected with AAV6-Caren. …”
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Physiological accumulation of lipid droplets in the newborn liver during breastfeeding is driven by TLR4 ligands by Wanderson Ferreira da Silva Júnior, Karen Marques de Oliveira Costa, Hortência Maciel Castro Oliveira, Maísa Mota Antunes, Kassiana Mafra, Brenda Naemi Nakagaki, Pedro Sérgio Corradi da Silva, Júlia Duarte Megale, Sarah Campos de Sales, Douglas Carvalho Caixeta, Mário Machado Martins, Robinson Sabino-Silva, Cristina Maria Pinto de Paula, Luiz Ricardo Goulart, Rafael Machado Rezende, Gustavo Batista Menezes

“…Finally, we investigated the role of Toll-like receptor 4 (TLR4) signaling in fat storage using knockout mice and cell-specific deletion strategies. …”
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Effective gene therapy for metachromatic leukodystrophy achieved with minimal lentiviral genomic integrations by Lucas Tricoli, Sunetra Sase, Julia L. Hacker, Vi Pham, Maxwell Chappell, Laura Breda, Stephanie N. Hurwitz, Naoto Tanaka, Carlo Castruccio Castracani, Amaliris Guerra, Zhongqi Hou, Lars Schlotawa, Karthikeyan Radhakrishnan, Matthew Hogenauer, Aoife Roche, John Everett, Frederic Bushman, Peter Kurre, Rebecca Ahrens-Nicklas, Laura A. Adang, Adeline L. Vanderver, Stefano Rivella

“…Three-month-old Arsa-knockout (KO) MLD mice transplanted with Arsa-KO bone marrow (BM) cells transduced with 0.6 VCN of EA1 demonstrated behavior and CNS histology matching wild-type (WT) mice. …”
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Targeting protein kinase C-α prolongs survival and restores liver function in sepsis: Evidence from preclinical models by Ling Xiong, Dustin Beyer, Na Liu, Tina Lehmann, Sophie Neugebauer, Sascha Schaeuble, Oliver Sommerfeld, Philipp Ernst, Carl-Magnus Svensson, Sandor Nietzsche, Sebastian Scholl, Tony Bruns, Nikolaus Gaßler, Markus H. Gräler, Marc Thilo Figge, Gianni Panagiotou, Michael Bauer, Adrian T. Press

“…Using a preclinical murine sepsis model, we found that both PKCα knockout and the use of a PKCα-inhibitor midostaurin successfully restored liver function without hindering the host’s response or ability to clear the pathogen, highlighting PKCα’s vital role in excretory liver failure. …”
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Cancer cells avoid ferroptosis induced by immune cells via fatty acid binding proteins by Maria Angelica Freitas-Cortez, Fatemeh Masrorpour, Hong Jiang, Iqbal Mahmud, Yue Lu, Ailing Huang, Lisa K. Duong, Qi Wang, Tiffany A. Voss, Claudia S. Kettlun Leyton, Bo Wei, Wai-Kin Chan, Kevin Lin, Jie Zhang, Efrosini Tsouko, Shonik Ganjoo, Hampartsoum B. Barsoumian, Thomas S. Riad, Yun Hu, Carola Leuschner, Nahum Puebla-Osorio, Jing Wang, Jian Hu, Michael A. Davies, Vinay K. Puduvalli, Cyrielle Billon, Thomas P. Burris, Philip L. Lorenzi, Boyi Gan, James W. Welsh

“…We worked with cell lines including PD1-sensitive, PD1-resistant, B16F10, and QPP7 glioblastoma cells, and conducted in vivo studies in syngeneic 129 Sv/Ev, C57BL/6, and conditional knockout mice with Rora deletion specifically in CD8+ T cells, Cd8 cre;Rora fl mice. …”
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Inhibition of S100A8/A9 ameliorates neuroinflammation by blocking NET formation following traumatic brain injury by Guihong Shi, Yiyao Cao, Jianye Xu, Bo Chen, Xu Zhang, Yanlin Zhu, Liang Liu, Xilei Liu, Luyuan Zhang, Yuan Zhou, Shenghui Li, Guili Yang, Xiao Liu, Fanglian Chen, Xin Chen, Jianning Zhang, Shu Zhang

“…Further experiments with PAD4 knockout mice confirmed that the reduction of NETs could substantially alleviate S100A8/A9-driven neuroinflammation. …”
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