Age-associated microglial transcriptome leads to diminished immunogenicity and dysregulation of MCT4 and P2RY12/P2RY13 related functions by Martin Škandík, Lara Friess, Guillermo Vázquez-Cabrera, Lily Keane, Kathleen Grabert, Mireia Cruz De los Santos, Mercedes Posada-Pérez, Austeja Baleviciute, Mathilde Cheray, Bertrand Joseph

“…Through the integration of in vitro cultured BV-2 microglia with primary microglia datasets, we identify critical molecular determinants of microglial cellular aging confirmed in human-aged brain tissue. …”
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TMEM135 deficiency improves hepatic steatosis by suppressing CD36 in a SIRT1-dependent manner by Arun Chhetri, Channy Park, Hyunsoo Kim, Laxman Manandhar, Chagtsalmaa Chuluunbaatar, Jaetaek Hwang, Xiaofan Wei, Gyuho Jang, Batching Chinbold, Hyug Moo Kwon, Sang-wook Lee, Raekil Park

“…This study aims to investigate the role of TMEM135 on regulating cellular lipid import in the liver. Methods: We used in vivo, ex vivo, and in vitro models of steatosis. …”
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A Multifunctional MIL-101-NH2(Fe) Nanoplatform for Synergistic Melanoma Therapy by Shang J, Chen Y, Wang F, Yang J, Li Y, Yang L, Liu X, Zhong Z, Yue C, Zhou M

“…Hydroxyl radical and reactive oxygen species generation capacities were quantified. Cellular studies using B16F10 cells assessed cytotoxicity, cellular uptake, migration inhibition, and colony formation suppression. …”
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Automated Synthesis and Initial Evaluation of (4′-Amino-5′,8′-difluoro-1′H-spiro[piperidine-4,2′-quinazolin]-1-yl)(4-[18F]fluorophenyl)methanone for PET/MR Imaging of Inducible Nit... by Skye Hsin-Hsien Yeh, Wen-Sheng Huang, Chuang-Hsin Chiu, Chuan-Lin Chen, Hui-Ting Chen, Dae Yoon Chi, Zhengxing Ge, Tsung-Hsun Yu, Pao-Yeh Wang, Yu-Yeh Kuo, Chun-Tse Hung, Geng-Ying Li, Chi-Wei Chang

“…In this study, we carefully defined a strategic plan to develop iNOS-targeted molecular PET imaging using (4′-amino-5′,8′-difluoro-1′H-spiro[piperidine-4,2′-quinazolin]-1-yl)(4-fluorophenyl)methanone ([18F]FBAT) as a tracer in a mouse model of lipopolysaccharide- (LPS-) induced brain inflammation. Methods. An in vitro model, murine microglial BV2 cell line, was used to assess the uptake of [18F]FBAT in response to iNOS induction at the cellular level. …”
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Atlas of multilineage stem cell differentiation reveals TMEM88 as a developmental regulator of blood pressure by Sophie Shen, Tessa Werner, Samuel W. Lukowski, Stacey Andersen, Yuliangzi Sun, Woo Jun Shim, Dalia Mizikovsky, Sakurako Kobayashi, Jennifer Outhwaite, Han Sheng Chiu, Xiaoli Chen, Gavin Chapman, Ella M. M. A. Martin, Di Xia, Duy Pham, Zezhuo Su, Daniel Kim, Pengyi Yang, Men Chee Tan, Enakshi Sinniah, Qiongyi Zhao, Sumedha Negi, Meredith A. Redd, Joseph E. Powell, Sally L. Dunwoodie, Patrick P. L. Tam, Mikael Bodén, Joshua W. K. Ho, Quan Nguyen, Nathan J. Palpant

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Targeting sphingosine 1-phosphate receptor 3 inhibits T-cell exhaustion and regulates recruitment of proinflammatory macrophages to improve antitumor efficacy of CAR-T cells agains... by Weiting Liao, Ge Gao, Xia He, Pei Shu, Qizhi Ma, Benxia Zhang, Diyuan Qin, Yongsheng Wang

“…The results indicated that the S1PR3 antagonist could significantly enhance the efficacy of murine EpCAM CAR-T cells in vitro and in vivo. Mechanistically, the S1PR3 antagonist improved CAR-T cell activation, regulated the central memory phenotype, and reduced CAR-T cell exhaustion in vitro. …”
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Insights into the mechanisms of serplulimab: a distinctive anti-PD-1 monoclonal antibody, in combination with a TIGIT or LAG3 inhibitor in preclinical tumor immunotherapy studies by Yizhou Zhang, Ruicheng Wei, Ge Song, Xinyi Yang, Mengli Zhang, Wei Liu, Aiying Xiong, Xuehan Zhang, Qianhao Li, Wan-Jen Yang, Chencheng Han, Rui Liu, Chen Hu, Qingyu Wang, Jun Zhu, Yongqiang Shan

“…We characterized the differences between serplulimab with approved PD-1/PD-L1 inhibitors (pembrolizumab and nivolumab) in terms of their binding features and functions in vitro and anti-tumor activity in vivo. Cellular pathways underlying the efficacy of serplulimab were also investigated. …”
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Effect of supplementing epinephrine in maturation media on the developmental competence of buffalo oocytes by Ali Husnain, Abdul Khaliq, Talha Ashraf, Abdul Rehman, Muhammad Yasir Zahoor, Muhammad Binyameen, Amjad Riaz

“… Epinephrine is a catecholamine that plays a vital role during cellular stress by scavenging free radicals. During in vitro maturation, oocytes experience stressful conditions that likely compromise their development. …”
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Micro Immune Response On-chip (MIRO) models the tumour-stroma interface for immunotherapy testing by Alice Perucca, Andrea Gómez Llonín, Oriol Mañé Benach, Clement Hallopeau, Elisa I. Rivas, Jenniffer Linares, Marta Garrido, Anna Sallent-Aragay, Tom Golde, Julien Colombelli, Eleni Dalaka, Judith Linacero, Marina Cazorla, Teresa Galan, Jordi Pastor Viel, Xavier Badenas, Alba Recort-Bascuas, Laura Comerma, Patricia Fernandez-Nogueira, Ana Rovira, Pere Roca-Cusachs, Joan Albanell, Xavier Trepat, Alexandre Calon, Anna Labernadie

“…Here we develop MIRO, a fully humanised in vitro platform to model the spatial organisation of the tumour/stroma interface and its interaction with immune cells. …”
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Wilms tumor primary cultures capture phenotypic heterogeneity and facilitate preclinical screening by Lisa Götz, Jenny Wegert, Alireza Paikari, Silke Appenzeller, Sabrina Bausenwein, Christian Vokuhl, Taryn D. Treger, Jarno Drost, Christin Linderkamp, Dominik T. Schneider, Karen Ernestus, Steven W. Warman, Jörg Fuchs, Nils Welter, Norbert Graf, Sam Behjati, Rhoikos Furtwängler, Manfred Gessler

“…Wilms tumors (WT) are characterized by variable contributions of blastemal, epithelial and stromal elements, reflecting their diverse cellular origins and genetic drivers. In vitro models remain rare, despite a growing need to better characterize tumor biology and evaluate new treatments. …”
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Synergistic Approach of High-Precision 3D Printing and Low Cell Adhesion for Enhanced Self-Assembled Spheroid Formation by Chunxiang Lu, Aoxiang Jin, Chuang Gao, Hao Qiao, Huazhen Liu, Yi Zhang, Wenbin Sun, Shih-Mo Yang, Yuanyuan Liu

“…Furthermore, the chip shows significant potential for real-time in vitro monitoring of cellular viability, reaction kinetics, and drug sensitivity, offering valuable advancements in biosensor technology for life sciences and medical applications.…”
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Protective Effect of Liposome-Encapsulated Glutathione in a Human Epidermal Model Exposed to a Mustard Gas Analog by Victor Paromov, Sudha Kumari, Marianne Brannon, Naga S. Kanaparthy, Hongsong Yang, Milton G. Smith, William L. Stone

“…In the EpiDerm, GSH-liposomes administered simultaneously or 1 hour after CEES exposure (2.5 mM) increased cell viability, inhibited CEES-induced loss of ATP and attenuated changes in cellular morphology, but did not reduce caspase-3 activity. …”
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Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy by Qiaoyun Gong, Jia’nan Xie, Yang Liu, Ying Li, Guanfang Su

“…Because microRNAs (miRNAs) represent effective DR therapeutic targets, we identified aberrantly expressed miRNAs associated with cellular dysfunction in early DR and detected their potential targets. …”
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Pyridoxal-5′-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway by Cong Li, Jinxian Huang, Huasu Zhu, Qing Shi, Dong Li, Xiuli Ju

“…Adipose-derived mesenchymal stem cells (A-MSCs) are promising cellular therapies for the treatment of immune-mediated diseases. …”
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The Role of PPARs in Lung Fibrosis by Heather F. Lakatos, Thomas H. Thatcher, R. Matthew Kottmann, Tatiana M. Garcia, Richard P. Phipps, Patricia J. Sime

“…Evidence is accumulating that the peroxisome proliferator-activated receptors (PPARs) play important roles in regulating processes related to fibrogenesis, including cellular differentiation, inflammation, and wound healing. …”
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Structural Characteristics and Inhibiting Oxidative Stress of Nine-steaming-nine-processing Polygonatum Kingianum Coll.et Hemsl Polysaccharides by LIU Yu-bin, CHEN Chang-an, SU Yi-lin, LUO Zhen, YAN Jin, LI Pan, DU Bing

“…Additionally, iodine-potassium iodide tests confirmed that NPKP is a highly branched polysaccharide. In vitro cellular experiments showed that NPKP significantly reduced reactive oxygen species (ROS) levels in HepG2 cells. …”
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Calreticulin as an Adjuvant In Vivo to Promote Dendritic Cell Maturation and Enhance Antigen-Specific T Lymphocyte Responses against Melanoma by Zheng Gong, Ming Chen, Jie Miao, Chao-Jie Han, Qiao Zhong, Fang-Yuan Gong, Xiao-Ming Gao

“…An endoplasmic reticulum resident protein, calreticulin (CRT), participates in many cellular processes. CRT is a tumor-associated antigen with an important role in antitumor immunity. …”
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A Pseudomonas-based bio-formulation to control bacterial blight of pomegranate caused by Xanthomonas axonopodis pv. punicae by Pavan Kumar, Giandomenico Corrado, Girigowda Manjunatha, Suma Ramegowda, Pradeep Kumar, Boris Basile, Ganadalu Puttaswamy Mutthuraju, Baggana Girish Jasmitha

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A Comparative Transcriptomic Analysis of Human Placental Trophoblasts in Response to Pathogenic and Probiotic Enterococcus faecalis Interaction by Qianglai Tan, Zhen Zeng, Feng Xu, Hua Wei

“…GO analysis revealed that these DEGs were mainly related to cellular and metabolic processes and biological regulation. …”
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Anticancer Activity of Ferulic Acid-Inorganic Nanohybrids Synthesized via Two Different Hybridization Routes, Reconstruction and Exfoliation-Reassembly by Hyoung-Jun Kim, Kitae Ryu, Joo-Hee Kang, Ae-Jin Choi, Tae-il Kim, Jae-Min Oh

“…Therefore we could conclude that the nanohybrids of ferulic acid and layered double hydroxide had cellular delivery property of intercalated molecules on cancer cell lines.…”
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