Differential Susceptibility to Propofol and Ketamine in Primary Cultures of Young and Senesced Astrocytes by Liang Huang, Ferit Tuzer, Abigail Murtha, Michael Green, Claudio Torres, Henry Liu, Shadi Malaeb

“…Oxidative stimulus was applied to commercially available fetal cell lines of human astrocytes in vitro to induce morphological changes in cellular senescence. …”
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Transcription Factor Lbx1 Expression in Mouse Embryonic Stem Cell-Derived Phenotypes by Stefanie Schmitteckert, Cornelia Ziegler, Liane Kartes, Alexandra Rolletschek

“…Here, we used mouse embryonic stem (ES) cells which have the capacity to develop into cells of all three primary germ layers. During in vitro differentiation, ES cells recapitulate cellular developmental processes and gene expression patterns of early embryogenesis. …”
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Using HBmito Crimson to Observe Mitochondrial Cristae Through STED Microscopy by Xichuan Ge, Wei Ren, Chunyan Shan, Peng Xi, Baoxiang Gao

“…Mitochondrial cristae, formed by folding the mitochondrial inner membrane (IM), are essential for cellular energy supply. However, the observation of the IM is challenging due to the limitations in spatiotemporal resolution offered by conventional microscopy and the absence of suitable in vitro probes specifically targeting the IM. …”
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Synthesis, Cytotoxicity, and Antileishmanial Activity of N,N'-Disubstituted Ethylenediamine and Imidazolidine Derivatives by Gustavo S. G. de Carvalho, Patrícia A. Machado, Daniela T. S. de Paula, Elaine S. Coimbra, Adilson D. da Silva

“…This paper describes the preparation of N,N'-disubstituted ethylenediamine and imidazolidine derivatives and their in vitro biological activities against Leishmania species. …”
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LDHA-mediated glycolysis in stria vascularis endothelial cells regulates macrophages function through CX3CL1-CX3CR1 pathway in noise-induced oxidative stress by Ying Yi, Min-Yu Wu, Kai-Tian Chen, An-Hai Chen, Lin-Qiu Li, Qin Xiong, Xian-Ren Wang, Wen-Bin Lei, Guan-Xia Xiong, Shu-Bin Fang

“…In this study, we investigated the effects of glycolysis on SV-ECs in vitro and on the SV in vivo. Our previous research identified the glycolysis pathway as a potential mechanism underlying the SV-ECs injuries induced by oxidative stress. …”
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NKX6.3 modulation of mitotic dynamics and genomic stability in gastric carcinogenesis by Jung Hwan Yoon, Jeong-Kyu Kim, Jung Woo Eun, Hassan Ashktorab, Duane T. Smoot, Suk Woo Nam, Won Sang Park

“…Mitotic integrity and genomic stability are crucial in maintaining cellular homeostasis and preventing tumorigenesis. …”
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OSM May Serve as a Biomarker of Poor Prognosis in Clear Cell Renal Cell Carcinoma and Promote Tumor Cell Invasion and Migration by Shuzhang Wei, Yin Chen, Xiaokai Shi, Li Zuo, Lifeng Zhang

“…Also, the univariate and multivariate Cox analyses of clinical characteristics show that OSM in ccRCC may be able to predict a poor prognosis on its own as a biomarker. In vitro cellular experiments demonstrated that high OSM expression had no discernible impact on ccRCC cell proliferation compared to the control group, but it did promote tumor cell invasion and migration. …”
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Telomerase RNA structural heterogeneity in living human cells detected by DMS-MaPseq by Nicholas M. Forino, Jia Zheng Woo, Arthur J. Zaug, Arcelia Gonzalez Jimenez, Eva Edelson, Thomas R. Cech, Silvi Rouskin, Michael D. Stone

“…We propose that this misfolded portion of the cellular hTR pool is either slowly refolded or degraded, suggesting that kinetic RNA folding traps studied in vitro may also hinder ribonucleoprotein assembly in vivo.…”
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Development of Delivery Systems Enhances the Potency of Cell-Based HIV-1 Therapeutic Vaccine Candidates by Amin Hadi, Abbas Rastgoo, Maryam Eskandarian, Nooshin Haghighipour, Azam Bolhassani

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Preparation of Ginsenoside Rg3 and Protection against H2O2-Induced Oxidative Stress in Human Neuroblastoma SK-N-SH Cells by Gang Li, Xiao-xiao Zhang, Lin Lin, Xiao-ning Liu, Cheng-jun Ma, Ji Li, Chi-bo Wang

“…MTT assay and LDH leakage assay were used to determine the cell viability and the oxidative stress cellular model was established by means of H2O2 (600 μM for 4 h). …”
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Antihyperglycemic Activity of Eucalyptus tereticornis in Insulin-Resistant Cells and a Nutritional Model of Diabetic Mice by Alis Guillén, Sergio Granados, Kevin Eduardo Rivas, Omar Estrada, Luis Fernando Echeverri, Norman Balcázar

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CRP and TNF-α  Induce PAPP-A Expression in Human Peripheral Blood Mononuclear Cells by Weiping Li, Hongwei Li, Fusheng Gu

“…Human PBMCs are capable of expressing PAPP-A in vitro, expression that may be regulated by CRP and TNF-α through the NF-κB pathway. …”
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FTO effects the proliferation, invasion, and glycolytic metabolism of colon cancer by regulating PKM2 by Kongyan Zhang, Fei Zhang, Jiahe Wang

“…Cellular behaviors such as proliferation, migration, invasion, and apoptosis were evaluated using various assays. …”
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Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets by Myungji Kim, Seungyeon Cho, Dong Gyu Hwang, In Kyong Shim, Song Cheol Kim, Jiwon Jang, Jinah Jang

“…Abstract Pancreatic islets are densely packed cellular aggregates containing various hormonal cell types essential for blood glucose regulation. …”
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Human Cardiac Fibroblast Number and Activation State Modulate Electromechanical Function of hiPSC-Cardiomyocytes in Engineered Myocardium by Cassady E. Rupert, Tae Yun Kim, Bum-Rak Choi, Kareen L. K. Coulombe

“…It is well accepted that heterotypic cellular interactions impact the phenotype of cardiomyocytes. …”
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CRMP4 and CRMP2 Interact to Coordinate Cytoskeleton Dynamics, Regulating Growth Cone Development and Axon Elongation by Minghui Tan, Caihui Cha, Yongheng Ye, Jifeng Zhang, Sumei Li, Fengming Wu, Sitang Gong, Guoqing Guo

“…Cytoskeleton dynamics are critical phenomena that underpin many fundamental cellular processes. Collapsin response mediator proteins (CRMPs) are highly expressed in the developing nervous system, mediating growth cone guidance, neuronal polarity, and axonal elongation. …”
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Pharmacokinetic studies and anticancer activity of curcumin-loaded nanostructured lipid carriers by Wang Fengling, Chen Jin, Dai Wenting, He Zhengmin, Zhai Dandan, Chen Weidong

“…In addition, the anticancer activity of Cur-NLC against human lung adenocarcinoma A549 cells was verified by a cellular uptake study, and a cytotoxicity and apoptosis assay. …”
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The Anti-Inflammatory Effect of Callicarpa nudiflora Extract on H. Pylori-Infected GES-1 Cells through the Inhibition of ROS/NLRP3/Caspase-1/IL-1β Signaling Axis by Lili Li, Bo Bao, Xingxing Chai, Xiaoyu Chen, Xiaohua Su, Shixiu Feng, Xiaohui Zhu

“…This study aims to examine the effect of CN on H. pylori-infected GES-1 cells in vitro and elucidate its underlying mechanism by extracting active ingredients from air-dried leaves. …”
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Golden Tandem of Photothermal Ablation and Simultaneous Anti-Inflammation in One Nanoparticle for Activated Macrophage-Targeted Atherosclerosis Treatment by Lu Y, Wang Y, Li Y, Li Y, Jiang YW, Li J

“…The preparation and characterization of GSNPD, their cellular toxicity, photothermal conversion and targeted ablation efficiency, anti-inflammation and ROS scavenging effect, as well as the inhibition of foam cell formation were studied in vitro.Results: The experimental results showed that the fabricated GSNPD NPs displayed positive effects on anti-atherosclerosis by pro-inflammatory macrophages ablation, NO production and ROS scavenging.Discussion: GSNPD NPs were designed to effectively and accurately ablate pro-inflammatory macrophages by recognizing and targeting to SR-A overexpressed on the activated macrophages of arterial plaques via PTT, and simultaneous inhibit the PTT-induced inflammation through the laser-activated NO release in situ. …”
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Epithelial and immune transcriptomic characteristics and possible regulatory mechanisms in asthma exacerbation: insights from integrated studies by Ye Liu, Yue Li, Ruhao Wu, Yu Wang, Pengfei Li, Tianci Jiang, Ke Wang, Yize Liu, Zhe Cheng

“…The results of the qRT-PCR demonstrated that the 9 key regulatory genes were involved in asthma.ConclusionsWe systematically explored epithelial and immune characteristics in asthma exacerbation and identified 9 key regulatory genes underlying asthma occurrence and progression, which may be valuable for providing new insights into the cellular and molecular mechanisms driving asthma exacerbations.…”
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