Optimization of chemical transfection in airway epithelial cell lines by Tony J. F. Guo, Wan Yi Liang, Gurpreet K. Singhera, Jasmine Memar Vaghri, Janice M. Leung, Del R. Dorscheid

“…Consideration and optimization of cell line and transfection conditions may be useful for improving nonviral genetic techniques in vitro.…”
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Vitamin D Potentiates the Inhibitory Effect of MicroRNA-130a in Hepatitis C Virus Replication Independent of Type I Interferon Signaling Pathway by Xiaoqiong Duan, Yujuan Guan, Yujia Li, Shan Chen, Shilin Li, Limin Chen

“…Calcitriol, the bioactive metabolite of vitamin D, was reported to inhibit HCV production in a synergistic fashion with interferon, a treatment in vitro. Our previous study established that miR-130a inhibits HCV replication by restoring the host innate immune response. …”
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Power Generation from Human Leukocytes/Lymphocytes in Mammalian Biofuel Cell by Güray Güven, Pablo Lozano-Sanchez, Arcan Güven

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Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice by Yuqing Sun, Jingfei Fu, Feiran Lin, Shengnan Li, Juan Du, Yi Liu, Yuxing Bai

“…Furthermore, the downstream cellular signaling pathway regulated by NO was also explored. …”
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Probiotics for the Control of Parasites: An Overview by Marie-Agnès Travers, Isabelle Florent, Linda Kohl, Philippe Grellier

“…In most cases, evidence for a beneficial effect was obtained by studies using animal models. In a few cases, cellular interactions between probiotics and pathogens or relevant host cells were also investigated using in vitro culture systems. …”
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RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori by Federico D’Agostino, Eva Pinatel, Alexandra Meynhardt, Vincenzo Scarlato, Andrea Vannini, Davide Roncarati

“…Summary: Ribonucleases are involved in several biological processes, including the turnover of structural and messenger RNAs and the specific processing of the cellular transcriptome. Here, we characterized the RNase Y from Helicobacter pylori. …”
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Dynamic localization of glucokinase and its regulatory protein in hypothalamic tanycytes. by Magdiel Salgado, Estefanía Tarifeño-Saldivia, Patricio Ordenes, Carola Millán, María José Yañez, Paula Llanos, Marcos Villagra, Roberto Elizondo-Vega, Fernando Martínez, Francisco Nualart, Elena Uribe, María de Los Angeles García-Robles

“…Thus, in tanycytes, GK can act as a molecular switch to arrest cellular responses to increased glucose.…”
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Contribution of In Vivo and Organotypic 3D Models to Understanding the Role of Macrophages and Neutrophils in the Pathogenesis of Psoriasis by Isabelle Lorthois, Daniel Asselineau, Nathalie Seyler, Roxane Pouliot

“…Current data from animal models and, to a lesser extent, three-dimensional in vitro models have confirmed the interest in leaning towards other immune cell types as a potential new cellular target for the treatment of the disease. …”
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Patient-Specific Pluripotent Stem Cells in Neurological Diseases by Serpen Durnaoglu, Sermin Genc, Kursad Genc

“…Patient-specific iPSC-based modeling of neurogenetic and neurodegenerative diseases is an emerging efficient tool for in vitro modeling to understand disease and to screen for genes and drugs that modify the disease process. …”
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Mechanistic Insights into the Inhibition of Colorectal Cancer by BuShenFang through Adenomatous Polyposis Coli Expression and Wnt/β-catenin Pathway Regulation by Khang Wen Goh, Jie Ji, Hiu Ching Phang, Xian Gu, Rajesh Sreedharan Nair, Xiaohui Wei, Mohammed Tahir Ansari, Kai Bin Liew

“…The study utilized HCT116 and SW620 CRC cell lines, employing in vitro experiments including cell viability assays, colony formation, flow cytometry, and Western blot to examine the influence of BSF on cellular proliferation, apoptosis, migration, and the Wnt/β-catenin signaling pathway. …”
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Enhanced Antitumor Efficacy and Reduced Toxicity in Colorectal Cancer Using a Novel Multifunctional Rg3- Targeting Nanosystem Encapsulated with Oxaliplatin and Calcium Peroxide by Xie Y, Zhu M, Bao H, Chen K, Wang S, Dai J, Chen H, Li H, Song Q, Wang X, Yu L, Pei J

“…The O2, H2O2, and Ca2+ generation ability of Rg3-Lip-OXA/CaO2 in solution and HCT116 cells were measured. The in vitro cellular uptake was observed via fluorescence microscope and flow cytometry. …”
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The transcription factor combination MEF2 and KLF7 promotes axonal sprouting in the injured spinal cord with functional improvement and regeneration-associated gene expression by Callan L. Attwell, Inés Maldonado-Lasunción, Ruben Eggers, Bastiaan A. Bijleveld, Ward M. Ellenbroek, Natascha Siersema, Lotte Razenberg, Dédé Lamme, Nitish D. Fagoe, Ronald E. van Kesteren, August B. Smit, Joost Verhaagen, Matthew R. J. Mason

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Brucella suis ΔmapB outer membrane vesicles as an acellular vaccine against systemic and mucosal B. suis infection by Florencia Muñoz González, Florencia Muñoz González, Magali G. Bialer, Maria L. Cerutti, Silvia M. Estein, Lila Y. Ramis, Pablo C. Baldi, Pablo C. Baldi, Ángeles Zorreguieta, Ángeles Zorreguieta, Mariana C. Ferrero, Mariana C. Ferrero

“…Immunogenicity was assessed by intramuscular immunization of mice with wt OMVs or ΔmapB OMVs, followed by the measurement of antigen-specific antibody levels and functional assays to evaluate the protective capacity of the antibodies. Cellular immunity was assessed by characterizing cytokine secretion through ELISA after in vitro stimulation of spleen cells with heat-killed B. suis. …”
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Activity-based chemical proteomics reveals caffeic acid ameliorates pentylenetetrazol-induced seizures by covalently targeting aconitate decarboxylase 1 by Guanjun Li, Ling Huang, Di Gu, Peili Wang, Letai Yi, Wenhua Kuang, Ying Zhang, Junzhe Zhang, Dandan Liu, Qiaoli Shi, Huan Tang, Jichao Sun, Guohua Zeng, Xin Peng, Jigang Wang

“…Additionally, a BV2 microglial cellular inflammation model was established by lipopolysaccharide stimulation. …”
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Anticancer effect of the antirheumatic drug leflunomide on oral squamous cell carcinoma by the inhibition of tumor angiogenesis by Chieko Niwata, Takayuki Nakagawa, Takako Naruse, Miyuki Sakuma, Nao Yamakado, Misaki Akagi, Shigehiro Ono, Kei Tobiume, Jing Gao, Eijiro Jimi, Kouji Ohta, Tomonao Aikawa

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Role of Fcγ receptors in HER2-targeted breast cancer therapy by Hope S Rugo, Antonino Musolino, William J Gradishar, Jeffrey L Nordstrom, Edwin P Rock, Fernanda Arnaldez, Mark D Pegram

“…These activities include induction of antibody-dependent cellular cytotoxicity (ADCC) and antibody-dependent cellular phagocytosis (ADCP), which are innate immune mechanisms of cancer cell elimination. …”
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The hypertrophic cardiomyopathy-associated A331P actin variant enhances basal contractile activity and elicits resting muscle dysfunction by Matthew H. Doran, Michael J. Rynkiewicz, Evan Despond, Meera C. Viswanathan, Aditi Madan, Kripa Chitre, Axel J. Fenwick, Duncan Sousa, William Lehman, John F. Dawson, Anthony Cammarato

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Identification of Sphingomyelinase on the Surface of Chlamydia pneumoniae: Possible Role in the Entry into Its Host Cells by Tuula A. Peñate Medina, Juha T. Korhonen, Riitta Lahesmaa, Mirja Puolakkainen, Oula Peñate Medina, Paavo K. J. Kinnunen

“…Because of the intriguing cellular and biochemical characteristics of the poorly understood entry of Chlamydia into its host cells these microbes were of particular interest. …”
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Inhibition of mutagenic activation of orthoaminoazotoluene increases its carcinogenicity for mouse liver by V. I. Kaledin, L. P. Ovchinnikova, S. I. Ilnitskaya, T. S. Morozkova, N. A. Popova

“…At the same time, a large number of carcinogens demonstrate positive activity in different in vitro tests for mutagenicity. These findings are indicative of a geno- toxic mechanism of carcinogen action. …”
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Potential Therapies by Stem Cell-Derived Exosomes in CNS Diseases: Focusing on the Neurogenic Niche by Alejandro Luarte, Luis Federico Bátiz, Ursula Wyneken, Carlos Lafourcade

“…Exosomes have recently been tested in vivo and in vitro as therapeutic conveyors for the treatment of diseases. …”
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