Simultaneous Dual-Color Fluorescence Microscope: A Characterization Study by Zheng Li, Xiaodong Chen, Liqiang Ren, Jie Song, Yuhua Li, Bin Zheng, Hong Liu

“…However, such class of microscopic systems functions differently from traditional fluorescence microscopes.…”
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Accessible and accurate cytometry analysis of adherent cells using fluorescence microscopes by Daniel Foyt, Yiming Kuang, Samma Rehem, Klaus Yserentant, Bo Huang

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Analyses of hyphal diversity in Trichosporonales yeasts based on fluorescent microscopic observations by Keita Aoki, Moriya Ohkuma, Takashi Sugita, Yuuki Kobayashi, Naoto Tanaka, Masako Takashima

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An AI-assisted fluorescence microscopic system for screening mitophagy inducers by simultaneous analysis of mitophagic intermediates by Yicheng Wang, Pengfei Song, Heqing Zhou, Pengwei Wang, Yan Li, Zhiyong Shao, Lu Wang, Yan You, Zuhai Lei, Jinhua Yu, Cong Li

“…Here, we introduce an artificial intelligence-assisted fluorescence microscopic system (AI-FM) that enables the uninterrupted recognition and quantification of key mitophagic intermediates by extracting mitochondrial pH and morphological features. …”
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Automatic fluorescence microscopic image analyzer: a novel AI-based tool for early diagnosing superficial fungal infections by Wenjing He, Chunjiao Zheng, Lianjuan Yang, Jingwen Tan

“…To improve diagnostic accuracy and efficiency, we evaluated the performance of a novel Artificial Intelligence (AI) -powered Fluorescence Microscopic Image Analyzer (FMIA) in the early detection of SFIs. …”
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Ultrabright quantum dots assisted in vivo NIR-II fluorescence microscopic imaging for brain metastases in triple-negative breast cancer by Yuxiang Gao, Chi Zhang, Lijun Zhu, Zhong Du, Rong Ma, Le Guo, Nuernisha Alifu, Xueliang Zhang

Subjects: “…NIR-II fluorescence microscopic imaging…”
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A protocol for loading Calcein-AM into extracellular vesicles from mammalian cells for clear visualization with a fluorescence microscope coupled to a deconvolution system. by María-Angélica Calderón-Peláez, Jaime E Castellanos, Myriam L Velandia-Romero

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Rapid detection and quantification of Nile Red-stained microplastic particles in sediment samples by Masashi Tsuchiya, Tomo Kitahashi, Yosuke Taira, Hitoshi Saito, Kazumasa Oguri, Ryota Nakajima, Dhugal J. Lindsay, Katsunori Fujikura

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A Forensic Approach in the Determination of Sequence of Strokes using Fluorescence Spectrometry by Vartika Khare, Anu Singla, Pawan Gupta

Subjects: “…fluorescence microscope…”
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Enhancing Biomarker Detection and Imaging Performance of Smartphone Fluorescence Microscopy Devices by Muhammad Ahsan Sami, Muhammad Nabeel Tahir, Umer Hassan

Subjects: “…smartphone fluorescence microscope (SFM)…”
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Simultaneous detection of Listeria monocytogenes and pathogenic Vibrio by dual peptide nucleic acid fluorescence in situ hybridization by WU Shan, LI Ke, FANG Yun, LOU Chengjie, YU Huizhen, ZHANG Mingzhe, SHUAI Jiangbing, ZHANG Xiaofeng

“…By the usage of a Leica DM 6000B fluorescence microscope, four kinds of fluorescence filter cubes I3, L5, N21 and Y3 were compared after labeled with FAM and Cy3 fluorescences. …”
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Using of green fluorescent reporter gene (GFP) to monitor the fate of Fusarium moniliforme mycoparasitized by Trichoderma viride by ZHU Ting-heng, WANG Wei-xia, WANG Chang-chun, YANG Rui-qin, CAI Xin-zhong

“…Fluorescence microscope observation indicated that attacked hyphae of F. moniliform were distorted, swollen or broken. …”
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A large field of view 2- and 3-photon microscope by Jack Waters

“…Abstract A new multiphoton fluorescence microscope has been developed, offering cellular resolution across a large field of view deep within biological tissues. …”
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Image contrast restoration in total internal reflection fluorescence microscopy by Samaneh Pahlevani, Batool Sajad, Sharareh Tavaddod

“…We applied the iterative restoration method of Richardson–Lucy on the image that was taken with a total-internal-reflection-fluorescence microscope and found it is a very efficient method in improving the contrast of images if, the signal level of fluorophore emission in an image is considered and in an area with a low signal level, the iteration number needs to be about three times more than the area with a high signal level.…”
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Cloning and eukaryotic expression of a new MSTN gene transcription of Muscovy duck by HAO Wen-bo, YANG Xiao-yu, LI Xin-hua, GU Wen-feng, HU Lan

“…The green fluorescence protein was detected with fluorescence microscope at 48 h after transfection, which was about 14 kDa. …”
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Phototriggerable 2',7-caged paclitaxel. by Radu A Gropeanu, Hella Baumann, Sandra Ritz, Volker Mailänder, Thomas Surrey, Aránzazu del Campo

“…Short light exposure under a fluorescence microscope allowed controlled delivery of free PTX during imaging.…”
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Role of autophagy in lipid aggregation in lipid-induced renal tubular epithelial damage by WANG Rui, ZHANG Ning

“…Objective To investigate the role of autophagy in lipid aggregation in lipid-induced renal tubular epithelial damage.Methods Renal tubular epithelial cells(HK-2) were incubated with low density lipoprotein(LDL) at different concentrations(0,20 μg/ml,50 fig/ml,and 100 μg/ml)for 24 h.Lipid deposition in HK-2 cells was evaluated by oil red O stain.Autophagosomes were observed by transmission electron microscopy.The LC3-GFP fusion protein was observed by fluorescence microscope.LC3 Ⅰ/Ⅱ protein was assessed by Western blotting.Results(1)LDL stimulate HK-2 cell for 24 h at the concentration in range from 0—100 μg/ml,and we detected lipid deposition in HK-2 cell stain by oil red O.The result showed that lipid deposition increasing caused by LDL stimulating in HK-2 cell performed in a time-dependent manner.(2) Autophagosome were observed in nomal HK-2 cell by transmission electron microscopy.However,after LDL stimulation HK-2 cell,autophagosome was increased in a dose-dependent manner with the LDL at the concentration from 0 to 50 μg/ml.But,autophagosome decreased rapidly at LDL 100 μg/ml concentration.(3) Few LC3-GFP dots were present in nomal HK-2 cell in basal condition,which were detected by fluorescence microscope.However,after LDL stimulation HK-2 cell,LC3-GFP dots was increased in a dose-dependent manner with the LDL at the concentration from 0 to 50 μg/ml(P<0.05).But,LC3-GFP dots decreased rapidly at LDL 100 μg/ml concentratioa(4)Both LC3- I and LC3-Ⅱ expressed in HK-2 cell,but LC3- I expression was dominant in normal cell.After LDL at concentration range from 0—50 μg/ml stimulated HK-2 cell,both LC3-Ⅰ and LC3-Ⅱ increased gradully,but LC3-Ⅱ increased more obviously.The expression of LC3- Ⅰ in 50 μg/ml LDL treated group was 2 times as that in 0 μg/ml LDL treated group(P<0.05).But the expression of LC3-Ⅱ in 50 μg/ml LDL treated group was 3 times as that in0 μg/ml LDL treated group(P<0.05).Thus,LC3-Ⅱ/LC3-I ratio increased in a dose-dependent manner at LDL concentration range from 0 to 50 μg/ml.However,when LDL concentration increased at 100 μg/ml,both LC3- Ⅰ and LC3-Ⅱ were drastically reduced.The expression of LC3- I in 100μg/ml LDL treated group was almost equally with that in 0 μg/ml LDL treated group(P>0.05).The expression of LC3- Ⅱ in 100 μg/ml LDL treated group dropped and it is only 1.5 times as that in 0 μg/ml LDL treated group(P<0.05).LC3-II/LC3-I ratio were also declined sharply.Conclusions Autophagy may be involved in lipid-induced renal tubular epithelial damage in lipid aggregation.…”
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Construction of DF-1 Cell Line of Stably Expressing Cas9 Protein Mediated by Lentivirus and Verification of Its Activity by Xiaojiao LI, Xinyu ZHU, Xian ZOU, Xia YAN, Yanhua HE, Chenglong LUO

“…Finally, the repair of mCherry expression by different stable transformants was analyzed by fluorescence microscope.【Result】27 DF-1 cell lines of stably expressing Cas9 protein were obtained after antibiotic screening, cell genome was stably transfected by PCR amplification, and the results showed that these cells contained Cas9 protein sequences. …”
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Synthesis and self-assembly behavior of amphiphilic diblock copolymer dextran-block-poly(&#949;-caprolactone) (DEX-b-PCL) in aqueous media

“…When these copolymers were allowed to self-assemble in aqueous solution, the morphology of assembled aggregates varied as a function of fDEX when characterized by transmission electron microscope (TEM), fluorescence microscope (FM) and dynamic laser scattering (DLS). …”
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Preparation and properties of polylactic acid-based composite fluorescent membrane based on layer-by-layer assembly by M. M. Wang, H. L. Li, M. R. Liu

“…After being repeatedly bleached 1000 times by confocal laser scanning fluorescence microscope, the fluorescence intensity of the PLA/(CNCs/Mg-Al-Eu LDHs)10 film remained 96%. …”
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