Closing the loop: establishing an autonomous test-learn cycle to optimize induction of bacterial systems using a robotic platform by Jan Benedict Spannenkrebs, Aron Eiermann, Thomas Zoll, Silke Hackenschmidt, Johannes Kabisch, Johannes Kabisch, Johannes Kabisch

“…The platform is shown to be able to automatically and autonomously optimize the inducer concentration for a Bacillus subtilis system and the combination of inducer and feed release for a Escherichia coli system. As a target product the readily measurable green fluorescent reporter protein (GFP) is produced over multiple, consecutive iterations of testing. …”
Get full text

Efficacy of DNA vaccine encoding koi herpesvirus glycoprotein GP-25in common carp juvenile by immersion by Soko Nuswantoro, . Alimuddin, Munti Yuhana, Ayi Santika, Sri Nuryati, Zakki Zainun, Mira Mawardi

“…A total of 400 common carp juveniles at 30-day-old were immersed in 1-L water containing 1.3×108CFU/mL of the killed Escherichia coli cells carrying DNA vaccine. Three frequencies and three duration of fish immersion were tested, namely: 1×30 minutes, 1×60 minutes, 1× 90 minutes, 2×90 minutes and 3×90 minutes by interval of 24 hours. …”
Get full text

Bioactive Properties of <i>Pentacalia vaccinioides</i> (Kunth) Cuatrec. (Asteraceae) Essential Oils: Evaluation of Antimicrobial and Antioxidant Activities by Luis G. Sequeda-Castañeda, María A. Castellanos-Gómez, Carlos L. A. Céspedes-Acuña

“…Additionally, the EOs demonstrated bactericidal activity against <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>, with MIC values of 5.0 and 45 µg/mL, respectively. <i>Escherichia coli</i> and <i>Pseudomonas aeruginosa</i> did not show antimicrobial susceptibility to EOs. …”
Get full text

Hydrogeochemistry of Surface and Ground Water in Alatening Village, Northwest Region, Cameroon by Kahnji Iren Njoyim, Lucas Kengni, Margaret Awah Tita, Estella Njoyim Buleng Tamungang, Vitalis Fonfo Fonzenyuy, Bertrand Tatoh Aziwo

“…Faecal coliforms as well as specific bacteria such as Enterobacter, Escherichia coli, Streptococcus, Salmonella, and Shigella were found in all the water samples, attributable to poor hygiene. …”
Get full text

Cross-contamination Risks in Dry Produce Packinghouses: Efficacy of Alcohol-based Sanitizers to Reduce Salmonella and Potential Surrogates on Relevant Surface Materials by Alexandra Nerney, Stuart Reitz, Jovana Kovacevic, Joy Waite-Cusic

“…Transfer of Salmonella and potential surrogates (Escherichia coli, Enterococcus faecium) to and from onions to FCS materials (high-density polypropylene, polyester-nylon conveyor belts, plywood) was quantified. …”
Get full text

Synthesis, Characterization, Cyclic Voltammetry, and Biological Studies of Co(II), Ni(II), and Cu(II) Complexes of a Tridentate Schiff Base, 1-((E)-(2-Mercaptophenylimino) Methyl)... by Maurice Kuate, Mariam Asseng Conde, Evans Ngandung Mainsah, Awawou G. Paboudam, Francis Merlin M. Tchieno, Kevin I. Y. Ketchemen, Ignas Tonle Kenfack, Peter T. Ndifon

“…In vitro antibacterial and antifungal activity against five bacterial strains (Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Enterococcus faecalis, and Proteus mirabilis) and five fungal strains (Candida albicans, Candida glabrata, Candida tropicalis, Candida krusei, and Candida parapsilosis) showed no antifungal activity but moderate antibacterial activity on E. coli, S. aureus, P. aeruginosa, and P. mirabilis bacterial strains. …”
Get full text

Rendimiento óptimo de larvas de moscas en varias cosechas con tres proporciones de salvado de trigo, cerdaza y gallinaza by Enrique Casanovas Cosío, Reina Reyna Reyes, Nelson Valladares Enriquez, Alexis Suárez del Villar Labastida, Joan Manuel Leyva Benavides

“…No se observó presencia de agentes patógenos: Salmonella spp., Escherichia coli, y Coccidia en los tratamientos. Palabras claves: Agua, Insectos, Proteína alternativa, Residuos orgánicos, Sustratos.…”
Get full text

Effects of Storage Temperature and Spices Incorporation on the Stability and Antibacterial Properties of Fontitrygon margarita (Günther, 1870) Liver Oil by Boris Simo Noutsa, Arlette Danelle Deutchoua Djitieu, Fabrice Fabien Dongho Dongmo, Fabrice Hervé Njike Ngamga, Sammuel Raymond Tchabong, Ousman Tamgue, Rosalie Anne Ngono Ngane, François Tchoumbougnang

“…The antibacterial activity of these oils gradually decreased during storage, with the minimal inhibitory concentrations (MICs) on bacterial strains of Escherichia coli (EC 137), Enterobacter cloacae (ENT 119 and ENT 51), and Yersinia enterocolitica (YERB 1) increasing from 16 to 128 mg/ml. …”
Get full text

Determination of In Vitro Antimicrobial Activity of Five Sri Lankan Medicinal Plants against Selected Human Pathogenic Bacteria by Manikkuwadura Hasara Nethmini De Zoysa, Hasanga Rathnayake, Ruwani Punyakanthi Hewawasam, Weerasinghe Mudiyanselage Dilip Gaya Bandara Wijayaratne

“…Plant extracts were screened against four clinically important Gram-positive and Gram-negative bacterial strains, Staphylococcus aureus (ATCC 25923), Escherichia coli (ATCC 25922), Pseudomonas aeruginosa (ATCC 27853), and Klebsiella pneumoniae (ATCC 700603). …”
Get full text

Change in Antimicrobial Therapy Based on Bronchoalveolar Lavage Data Improves Outcomes in ICU Patients with Suspected Pneumonia by Bharti Chogtu, Vrinda Mariya Elenjickal, Dharma U. Shetty, Mahsheeba Asbin, Vasudeva Guddattu, Rahul Magazine

“…Distribution of microorganisms based on BAL reports was as follows: Acinetobacter baumanii 45 (30%), Klebsiella pneumoniae 37 (24.66%), Escherichia coli 9 (6%), and Pseudomonas aeruginosa 9 (6%). …”
Get full text

Antibiofilm and Anti-Quorum Sensing Potential of Safely-Synthesized Hydrated Zirconium Oxide-Coated Alginate Beads against Some Pathogenic Bacteria by Alfred Ngenge Tamfu, Yasemin Ispirli Dogaç, Ozgur Ceylan, Andreea Botezatu Dediu, Selahattin Bozkurt, Rodica Mihaela Dinica

“…Antimicrobial activity was evaluated against Staphylococcus aureus, Enterococcus faecalis, Listeria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Candida albicans, and Candida tropicalis, and MIC values ranged from 1.25 mg/mL to 10 mg/mL. …”
Get full text

Chemical Constituents and Antimicrobial and Antioxidant Activities of Essential Oil from Dried Seeds of Xylopia aethiopica by Samba Fama Ndoye, Yoro Tine, Insa Seck, Lalla Aicha Ba, Seydou Ka, Ismaila Ciss, Abda Ba, Seynabou Sokhna, Moussa Ndao, Rokhaya Sylla Gueye, Nango Gaye, Abdoulaye Diop, Jean Costa, Julien Paolini, Matar Seck

“…The antimicrobial activity of this essential oil was studied using disk diffusion and broth microdilution methods on four bacteria (Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa) and one fungus (Candida albicans). …”
Get full text

Heterologous Expression and Functional Analysis of <i>Exiguobacterium</i> Algin Lyase Gene by <i>Pichia pastoris</i> by Hanwen Wu, Kai Hou, Yutong Jiang, Mingjian Luan, Yuxia Sun, Xi He, Xiangzhong Zhao

“…Nevertheless, the expression of three genes in <i>Escherichia coli</i> revealed a lower alginate lyase activity compared to that of the original strain. …”
Get full text

Segurança microbiológica de hortaliças orgânicas no Brasil by Karina Rosa Morgado, Mariana Guedes Resende da Silva, Rafaela Anjos Fagundes Evaristo, Roger Santos Souza, Hilana Ceotto Vigoder

“…Dentre os grupos bacterianos mais frequentemente associadoas a DTAs pode-se destacar duas espécies bacterianas pertencentes à família Enterobacteriacea, Escherichia coli e Salmonella spp., além de Staphylococcus e Listeria monocytogenes. …”
Get full text

Discovery and Characterization of Novel Esterases with Ethyl Carbamate-Hydrolyzing Activity by LIU Qingtao, ZHU Sibao, WANG Tianwen, LI Chuang, QIAN Senhe, ZHANG Wenqing, CHENG Fan, TIAN Shufang

“…In this study, four esterases (ES1, ES5, ES8 and ES9) with relatively high ethyl carbamate (EC)-hydrolyzing activity at both pH 4.5 and 7.0 were identified from the NCBI database, and their soluble expression in recombinant Escherichia coli was achieved. Then, the expressed enzymes were purified and characterized. …”
Get full text

EVALUATION OF THE APPLICABILITY OF THE O.K.N.V.I. RESIST-5 AND THE KPC&MBL&0XA-48 DISK TESTS IN A ROUTINE MICROBIOLOGY LABORATORY by Stefana Sabtcheva, Sylvia Georgieva

“…RESIST-5 correctly detected all 56 carbapenemases, including KPC-2 in Klebsiella pneumoniae; OX-48 in Serratia marcescens, Citrobacter freundii, Enterobacter hormaechei and K. pneumoniae; NDM-1 in Escherichia coli, Morganella morganii, E. hormaechei, C. freundii, S. marcescens and K. pneumoniae; VIM-1 in Proteus mirabilis; VIM-4 in C. freundii and S. marcescens; VIM-86 with and without NDM-1 in Providencia stuartii, and NDM-5 with and without OXA-232 in K. pneumoniae. …”
Get full text

Antibacterial Additive for Polystyrene Based on Silver Nanoparticles Supported on Titanium Dioxide by Miguel A. Waldo-Mendoza, Lucía Martínez-Jothar, Socorro Oros-Ruiz, E. G. Villabona-Leal, Luis Manuel Céspedes-Covarrubias, Zoe V. Quiñones-Jurado, Eduardo Ortega, Elías Pérez

“….%) of the additive were made using an extrusion-injection molding process, and they were evaluated for their antibacterial properties against Escherichia coli using the Pour Plate method. Particles were distributed on the PS surface, and PS pieces presented a good antibacterial efficiency at 100, 300, and 500 ppm and decreased for 700 ppm due to an additive agglomeration on the PS surface. …”
Get full text

Engineering a promiscuous prenyltransferase for selective biosynthesis of an undescribed bioactive cannabinoid analog by Qin Yan, Yue-Gui Chen, Xiao-Wen Yang, An Wang, Xiao-Ping He, Xue Tang, Hong Hu, Kai Guo, Zong-Hua Xiao, Yan Liu, Sheng-Hong Li

“…This study introduces a fungal aromatic prenyltransferase AscC into the engineered Escherichia coli to catalyze the transfer of C5-C15 terpenoid linear precursors to olivetolic acid. …”
Get full text

Update of the list of qualified presumption of safety (QPS) recommended microbiological agents intentionally added to food or feed as notified to EFSA 21: Suitability of taxonomic... by EFSA Panel on Biological Hazards (BIOHAZ), Ana Allende, Avelino Alvarez‐Ordóñez, Valeria Bortolaia, Sara Bover‐Cid, Alessandra De Cesare, Wietske Dohmen, Laurent Guillier, Liesbeth Jacxsens, Maarten Nauta, Lapo Mughini‐Gras, Jakob Ottoson, Luisa Peixe, Fernando Perez‐Rodriguez, Panagiotis Skandamis, Elisabetta Suffredini, Marianne Chemaly, Pier Sandro Cocconcelli, Pablo Salvador Fernández Escámez, Miguel Prieto Maradona, Amparo Querol, Lolke Sijtsma, Juan Evaristo Suarez, Ingvar Sundh, Angela Botteon, Barizzone Fulvio, Sandra Correia, Lieve Herman

“…Of 54 microorganisms notified to EFSA between April and September 2024 (33 as feed additives, 17 as food enzymes or additives, 4 as novel foods), 50 were not evaluated because: 12 were filamentous fungi, 1 was Enterococcus faecium and 8 were Escherichia coli (all excluded from the QPS evaluation), and 29 were TUs that already have a QPS status. …”
Get full text

Point Mutations in the folP Gene Partly Explain Sulfonamide Resistance of Streptococcus mutans by W. Buwembo, S. Aery, C. M. Rwenyonyi, G. Swedberg, F. Kironde

“…A set of recombinant plasmids carrying different point mutations in cloned folP were separately transformed into folP-deficient Escherichia coli. Using sulfonamide-containing media, we assessed the growth of folP-deficient bacteria harbouring plasmids with differing folP point mutations. …”
Get full text