Fishy Business—Assessing the Efficacy of Active and Passive eDNA to Describe the Fish Assemblage of a River in Southwestern Western Australia to Support Effective Monitoring by Emma R. Stevens, Josephine Hyde, Leah S. Beesley, Daniel C. Gwinn, Suzanne Thompson, Lenore Morris, Paige R. Wilson, Deirdre B. Gleeson

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Hydrodynamics and Aquatic Vegetation Drive Spatial Patterns of Environmental DNA in Ponds by Sandra R. Mayne, Jeffrey A. Manning, Stephen M. Henderson, Meghan B. Parsley, Katherine M. Strickler, Jeffrey R. Nielson, Caren S. Goldberg

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Temporal Study of Environmental DNA and Acoustic Data Reveals Coexistence of Sympatric Bat Species in a North American Ecosystem by Vrinda M. Suresh, Trevor Hébert, Kirsten Isabel Verster, Elizabeth A. Hadly

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Roaming the Seas—Assessing Marine Invertebrate Biodiversity Along Salinity Gradients With Zooplankton and eDNA Metabarcoding by Alica Ohnesorge, Uwe John, Lucie Kuczynski, Stefan Neuhaus, Kingsly Chuo Beng, Bernd Krock, Silke Laakmann

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Rare Taxa Are Key Links in Regional Antimicrobial Resistance Profiles in Dusts Across Diverse North American Regions by Paul B. L. George, Florent Rossi, Marc Veillette, Amélia Bélanger Cayouette, Samantha Leclerc, Cindy Dumais, Nathalie Turgeon, Caroline Duchaine

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Temporal and Spatial eDNA Analysis of Fish Assemblages in Postindustrial, Urban Coastal Habitats by Alice Cunnington, Erika Neave, Peter Shum, Rupert Collins, Stefano Mariani

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Bottom Trawling and Multi‐Marker eDNA Metabarcoding Surveys Reveal Highly Diverse Vertebrate and Crustacean Communities: A Case Study in an Urbanized Subtropical Estuary by Jack Chi‐Ho Ip, Hai‐Xin Loke, Sam King Fung Yiu, Meihong Zhao, Yixuan Li, Yitao Lin, Chun‐Ming How, Jiezhang Mo, Meng Yan, Jinping Cheng, Vincent Chi‐Sing Lai, Leo Lai Chan, Kenneth Mei Yee Leung, Jian‐Wen Qiu

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Fish Gut Metabarcoding Detects Diets and Parasites Simultaneously by Dilli Prasad Rijal, Per Arneberg, Tanja Hanebrekke, Torild Johansen, Daniela Sint, Michael Traugott, Mette Skern‐Mauritzen, Jon‐Ivar Westgaard

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Ebbs and Flows of Marine Biodiversity: Navigating Spatiotemporal Patterns of Environmental DNA in a Coastal Tidal Ecosystem by Michelle Scriver, Ulla vonAmmon, Xavier Pochon, Martin Zirngibl, Francois Audrezet, Kaeden Leonard, Kylie Pedersen, Nick Bamford, Ngairo Tahere, Jo‐Ann L. Stanton, Neil J. Gemmell, Anastasija Zaiko

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eDNA Metabarcoding Analysis of Bony Fish Identification in Coastal Water of Gulf of Maine Using Nested Polymerase Chain Reaction of 12S rRNA Universal Primers by Bo‐Young Lee, Grant A. Milne, Corwin Freedman, Jenifer Miksis‐Olds, Bonnie L. Brown

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Using Gap Visualization to Navigate Multivariate Metabarcode Data, Select Primer Pairs, and Enhance Reference Data Quality by Xin‐Yi Chua, Louise Ord, Stephen J. Bent, David Lovell, Annette McGrath

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Evaluating Sampling Designs to Survey Fish Diversity in Lakes From Northern Temperate Zones by Erik García‐Machado, Eric Normandeau, Guillaume Côté, Isabeau Caza‐Allard, Charles Babin, Louis Bernatchez

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Monitoring of Endangered Freshwater Mussels in Sweden Using Digital PCR by Per Sundberg, Alizz Axberg, Nauras Daragmeh, Niklas Wengström, Marina Panova

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How, What, and Where You Sample Environmental DNA Affects Diversity Estimates and Species Detection by Anish Kirtane, Leif Howard, Caitlin E. Beaver, Margaret E. Hunter, Gordon Luikart, Kristy Deiner

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eDNA Metabarcoding, a Promising Tool for Monitoring Aquatic Biodiversity in the Estuaries of Reunion Island (South‐West Indian Ocean) by Lou‐Anne Jannel, François Guilhaumon, Pierre Valade, Pascale Chabanet, Guillaume Borie, Henri Grondin, Philippe Jourand

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Mapping Biodiversity Coast‐to‐Coast‐to‐Coast Across Canada's Three Oceans Using eDNA Metabarcoding by Loïc Jacquemot, Brian P. V. Hunt, Shaorong Li, Angela D. Schulze, Christoph M. Deeg, Ben J. G. Sutherland, Amy Tabata, Connie Lovejoy, Kristina M. Miller

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The Origin of the Matter Matters: The Influence of Terrestrial Inputs on Coastal Benthic Microeukaryote Communities Revealed by eDNA by Mathisse Meyneng, Raffaele Siano, Naïna Mouras, Dominique Ansquer, Christine Laporte‐Magoni, Florence Antypas, Thomas Haize, Hugues Lemonnier

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Assessment of Allometry in Environmental DNA and RNA Production From Ayu (Plecoglossus altivelis) in an Experimental Condition Using Mitochondrial and Nuclear Gene Markers by Toshiaki S. Jo, Yusuke Ozaki, Nao Matsuda, Hiroki Yamanaka

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Complementary Non‐invasive Fish Monitoring Distinguishes Depth‐Dependent Fish Communities by Ewan Burns, Vijini Mallawaarachchi, Thomas M. Clarke, Belinda Martin, Joseph D. DiBattista, Jamie Hicks, Danny Brock, Elizabeth A. Dinsdale, Charlie Huveneers, Michael P. Doane

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Experimental Evidence of Rapidly Decaying Environmental DNA Highlights Infection Risk from Two Major Amphibian Pathogens by Joseph D. Trafford, Trenton W. J. Garner, David J. Murrell, Julia J. Day

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