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341
Ophiostomatoid fungi associated with mites phoretic on bark beetles in Qinghai, China
Published 2020-07-01“…Mites were identified based on morphological characteristics, and fungi based on DNA sequences of four gene regions. In total, 173 mite individuals were collected belonging to 18 species in 11 genera. …”
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342
Negative heterosis for meiotic recombination rate in spermatocytes of the domestic chicken Gallus gallus
Published 2021-10-01“…The decrease in recombination rate in F1 is probably determined by difficulties in homology matching between the DNA sequences of genetically divergent breeds. The suppression of recombination in the hybrids may impede gene flow between parapatric populations and therefore accelerate their genetic divergence.…”
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343
Multi-Host of Ectomycorrhizal Fungi on Dipterocarpaceae in Tropical Rain Forests
Published 2019-05-01“…The identity of the ectomycorrhizal fungi was obtained by matching the samples’DNA sequence to the Genbank database. Based on the identification results, ectomycorrhizal fungi that associate with dipterocarpaceae on tree and seedling levels have genetic relationship with Dothideomycetes class and Sordariales, Sebacinales, Cantharellales, Russulales, Agaricales, Boletales, and Thelephorales orders. …”
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344
Fiddler Crabs (Crustacea: Decapoda: Ocypodidae) From Coastal Ecuador and the Galápagos Islands: Species Descriptions and DNA Barcodes
Published 2025-01-01“…A neighbor‐joining tree was constructed using the Kimura 2‐parameter model with a partial DNA sequence of the cytochrome oxidase‐subunit 1 gene (COI) for a barcoding study. …”
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345
Gene Polymorphism of Antigen B Subunit 2 and Pathogenesis of Cystic Echinococcosis in Murine Model
Published 2025-02-01“…Minor sequence polymorphisms, 1.67%, are observed in one of the isolates, whereas remarkable DNA sequence polymorphisms are noticed in three of the isolates. …”
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346
New insights into the stipitate hydnoid fungi Sarcodon, Hydnellum, and the formerly informally defined Neosarcodon, with emphasis on the edible species marketed in Southwest China
Published 2024-03-01“…In the present study, specimens collected in the field and purchased from the markets in Southwest China were analyzed based on morphological characters and DNA sequences. Phylogeny of the traditional Sarcodon s. lat. and Hydnellum s. lat. was reconstructed from the combined internal transcribed spacer (ITS), nuclear large ribosomal subunit (nLSU) and RNA polymerase II second largest subunit (RPB2) dataset based on expanded samples to reevaluate the taxonomic placements of the two genera. …”
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347
Identification of <i>Tinomiscium petiolare</i> from Vietnam using the DNA barcode
Published 2021-07-01“…In recent years, DNA barcoding has been known to be a fast and sensitive method for identifying species at any stage of development, using short DNA sequences. In this study we have performed the identification of T. petiolare specimens in Vietnam based on the sequence analysis of 4 DNA barcode loci: ITS, matK, rbcL and rpoC.Materials and methods. …”
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348
Transitioning from wet lab to artificial intelligence: a systematic review of AI predictors in CRISPR
Published 2025-02-01“…Abstract The revolutionary CRISPR-Cas9 system leverages a programmable guide RNA (gRNA) and Cas9 proteins to precisely cleave problematic regions within DNA sequences. This groundbreaking technology holds immense potential for the development of targeted therapies for a wide range of diseases, including cancers, genetic disorders, and hereditary diseases. …”
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349
Identification of species in the genus Nitraria L. (Nitrariaceae) based on nucleotide variability of nuclear ribosomal DNA
Published 2020-08-01“…Intragenomic polymorphism of ITS1 and ITS2 of nuclear ribosomal DNA sequences was analysed in 33 samples belonging to the Nitraria species N. schoberi, N. sibirica, and N. komarovii. …”
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350
Phage display as a tool for identifying HIV-1 broadly neutralizing antibodies
Published 2021-09-01“…Each phage particle in a library displays peptides or proteins fused to its coat protein and simultaneously carries the DNA sequence encoding the displayed peptide/protein in its genome. …”
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351
Transgenerational epigenetic heritability for growth, body composition, and reproductive traits in Landrace pigs
Published 2025-01-01“…Epigenetics is an important source of variation in complex traits that is not due to changes in DNA sequences, and is dependent on the environment the individuals are exposed to. …”
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352
Design and expression of a chimeric recombinant antigen (SsIR-Ss1a) for the serodiagnosis of human strongyloidiasis: Evaluation of performance, sensitivity, and specificity.
Published 2024-07-01“…The construct was expressed into the pET-23a (+) expression vector and the optimized DNA sequences of SsIR-Ss1a (873 bp) were cloned into competent E. coli DH5α cells. …”
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353
Variation in Botryosphaeriaceae from Eucalyptus plantations in YunNan Province in southwestern China across a climatic gradient
Published 2020-10-01“…Isolates were identified by comparing DNA sequences of the internal transcribed spacer ribosomal RNA locus (ITS), partial translation elongation factor 1-alpha (tef1), β-tubulin 2 (tub2) and DNA-directed RNA polymerase II subunit (rpb2) genes, and combined with their morphological characteristics. …”
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354
A positive experience in applying the biolistic approach to potato varieties Aksor and Nevskiy
Published 2021-04-01“…The main advantages of this method include the ability to simultaneously incorporate several target genes into the plant genome, carry out transfer without unnecessary agrobacterial parts and plasmid DNA sequences, and the short time needed to produce transgenic cells. …”
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355
High and Low Taxa Specificity of Rhizosphere Bacteria Communities of Mangrove (Rhizophora mucronata) from Kuala Langsa and Telaga Tujuh Island, East Aceh
Published 2024-05-01“…This research examines sediment from rehabilitated and natural areas to understand how human and natural factors shape bacterial communities.The DNA sequence was analyzed using Next-Generation Sequencing (NGS), which targeted the 16S bacterial region in the V3-V4 rDNA. …”
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356
Molecular Identification and Antimicrobial Potential of Streptomyces Species from Nepalese Soil
Published 2020-01-01“…In contrast, some Streptomyces species shared identical DNA sequences in multiple copies of 16S rRNA. The sequencing of numerous copies of 16S rRNA is not necessary, and the molecular sequencing of this region is not sufficient for the identification of bacterial species. …”
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357
Structural Characterization of B-DNA d(CGTGAATTCACG)<sub>2</sub> in Complex with the Specific Minor Groove Binding Fluorescent Marker Hoechst 33342
Published 2024-12-01“…This work focuses on the binding interactions of Hoechst 33342 with the specific DNA sequence d(CGTGAATTCACG)<sub>2</sub>. The structure of the complex was determined using single-crystal X-ray diffraction at a resolution of 1.9 Å in the space group <i>P</i>2<sub>1</sub>2<sub>1</sub>2<sub>1</sub>. …”
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358
Identification and quantification of transposable element transcripts using Long-Read RNA-seq in Drosophila germline tissues
Published 2024-09-01“…Transposable elements (TEs) are repeated DNA sequences potentially able to move throughout the genome. …”
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359
Nisin Z Production by Wild Strains of Lactococcus lactis Isolated from Brazilian (Italian Type) Fermented Sausage
Published 2020-01-01“…The five isolates produced different amounts of the bacteriocin, L. lactis ID1.5 and ID8.5 isolates being the best nisin Z producers. DNA sequence analysis did not reveal any sequence differences in the nisZ and nisF promoter regions that could explain the differences in nisin Z production, suggesting that there should be other factors responsible for differential nisin Z production by the isolates.…”
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360
Genetic variation of the nuclear sequences of mitochondrial origin associated with retrotransposon Tv1 insertions in Drosophila species of the virilis group
Published 2018-11-01“…Mitochondrial DNA sequences integrated into chromosomes are a promising object for designing genetic markers for studies of phylogenesis and genomic instability. …”
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