Protocol for isolating extracellular vesicles from human or mouse airway macrophages for functional assays and in vivo or in vitro experimentation by Ezgi Sari, Robert P. Richter, Delores A. Stacks, Crystal Lewis, Julian B. Smith, Ningyong Xu, Dakota C. Finley, Kristopher R. Genschmer, Camilla Margaroli

Subjects: “…Cell isolation…”
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Protocol for isolating cervico-vaginal fluid cells from Macaca mulatta to study immunological and functional changes during pregnancy by Daniel Short, Monica Cappelletti, Anne Vu, Mark R. Johnson, Suhas G. Kallapur, Pietro Presicce

Subjects: “…Cell isolation…”
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CURRENT METHODS IN ISOLATION OF HEMATOPOIETIC STEM CELL by Nilgün Okşak, Dürdane Serap Kuruca

Subjects: “…stem cell isolation…”
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Protocol for phenotyping mouse myeloid and lymphoid cells by mass cytometry by Laura Morin, Brice Autier, Patrice Hemon, Simon Le Gallou, Mikael Roussel, Sarah Dion, Valérie Lecureur

Subjects: “…cell isolation…”
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Protocol for characterizing craniopharyngioma subtypes and their microenvironments using single-cell RNA sequencing and immunohistochemistry by Takashi Kono, Tatsuma Matsuda, Masanori Fujimoto, Yuki Taki, Ikki Sakuma, Naoko Hashimoto, Yasuhiro Nakamura, Kentaro Horiguchi, Yoshinori Higuchi, Atsushi Onodera, Takashi Miki, Tomoaki Tanaka

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Protocol for generating and characterizing a nasal epithelial model using imaging with application for respiratory viruses by Victor H.K. Lam, Aleena Ghafoor, Yazan Khan, Shirley Constable, Lane B. Buchanan, David Zuanazzi, Reeya Parmar, Zeynep G. Tepe, Leigh J Sowerby, Cindy M. Liu, Ryan M. Troyer, Jessica L. Prodger

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Protocols to co-culture human primary lung cells in the simple-flow device by Cinta Iriondo, Sem Koornneef, Kari-Pekka Skarp, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Robbert J. Rottier

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Impact of enzymatic isolation on the propagation efficiency of patient-derived colorectal cancer organoids by Gizem Calibasi-Kocal, Tolga Sever, Aras Emre Canda, Leman Evren Kadioglu, Halil Ates, Yasemin Basbinar, Ender Ellidokuz

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Automatic Single-Cell Harvesting for Fetal Nucleated Red Blood Cell Isolation on a Self-Assemble Cell Array (SACA) Chip by Hsin-Yu Yang, Che-Hsien Lin, Yi-Wen Hu, Chih-Hsuan Chien, Mu-Chi Huang, Chun-Hao Lai, Jen-Kuei Wu, Fan-Gang Tseng

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Protocol for generating brain metastatic tumor cells through repeated intracardiac injections in mice by Li Wang, Xiaoxia Chen, Chengtao Dong, Shengqi Yin, Li Liang, Aidong Zhou

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Protocol for the isolation of brain microvessels and visualization of RNA fluorescence in mice and humans by Olivia M. Osborne, Oandy Naranjo, Silvia Torices, Sarah Schmidlin, Destiny Tiburcio, Minseon Park, Michal Toborek

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Protocol for isolation of endothelial cells from adult mouse brain by Audrey G. Miglietta, Jennifer David-Bercholz, Roeben N. Munji, Robert S. Pulido, Allison L. Soung, Niccolò Terrando, Richard Daneman, Ting Yang

Subjects: “…Cell isolation…”
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Evaluation the in-vitro magnesium oxide nanoparticles effect on morphological changes in rat alveolar pneumocyte cells by A Barkhordari, SM Jafari, SH Hekmatimoghaddam, A Jebali, M Esmaili, M Poorrajab, M Askarshahi, M Noorani, B fazli

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Fibromodulin-overexpressing fibroblast cells increase wound contraction, improve scar quality and enhance angiogenesis: an in-vivo study by Negar Abdollahzadeh, Mehran Vatanchian, Fatemeh Oroojalian, Seyed Ehsan Enderami, Amir Amani, Reza Salarinia

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Protocol for ex vivo culture of adult mouse testis tissue using a gel-based approach by Nadia Krarup Knudsen, Christine Hjorth Andreassen, Martin Blomberg Jensen, Anne Jørgensen

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Protocol for the biofabrication of immunocompetent tumor-on-chip models from patient solid tumors for assessment of anticancer therapies by Martin Nurmik, Ségolène Ladaigue, Isabella Hofer, Auriane Debache, Irina Veith, Fatima Mechta-Grigoriou, Stephanie Descroix, Gerard Zalcman, Maria Carla Parrini

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Protocol for the isolation and characterization of murine hematopoietic stem and progenitor cell-derived extracellular vesicles by Federica Zanotti, Ilaria Zanolla, Massimo Bonora, Claudia Morganti, Barbara Zavan, Letizia Ferroni, Paolo Pinton, Keisuke Ito

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Protocol for in vivo lineage tracing of regeneration-associated macrophages from injured skeletal muscle of adult mice by Neuza S. Sousa, Pedro Sousa-Victor, Joana Neves

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Single-cell RNA sequencing of intramedullary canal tissue to improve methods for studying fracture repair biology by James M Dominguez, Sharon M Moe, Neal X Chen, Todd O McKinley, Krista M Brown, Yunlong Liu, Hongyu Gao, Roman M Natoli

Subjects: “…cell isolation…”
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Protocol to isolate dorsal root ganglion neurons from embryonic rats by immunopanning and characterize them using RNAscope and immunofluorescence by Samuel Faraguna, Karina A. Peña, Husniye Kantarci

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