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161
The necessity of cell banks
Published 2017-02-01“…In addition, a recently developed technology of directed genome modification, CRISPR/Cas, allows correction of genetic mutations in iPSCs. …”
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162
Structural basis for RNA-guided DNA degradation by Cas5-HNH/Cascade complex
Published 2025-02-01“…Abstract Type I-E CRISPR (clustered regularly interspaced short palindromic repeats)–Cas (CRISPR-associated proteins) system is one of the most extensively studied RNA-guided adaptive immune systems in prokaryotes, providing defense against foreign genetic elements. …”
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163
Huntington’s disease modeling on HEK293 cell line
Published 2017-12-01“…The new genome editing approaches, such as CRISPR/Cas9, allow us to generate isogenic cell lines that can be useful for drug screening and studying mechanisms of molecular and cellular events triggered by certain mutation on an equal genetic background. …”
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164
A versatile cell line for establishing potency of cell type-specific AAV transgenes
Published 2025-03-01“…Here, we present the CRISPR activation-AAV receptor (CRAAVR) cell line, a stable HEK293T-derived line that overcomes both problems. …”
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165
Colon Cancer Genetic and Epigenetic Changes, Etiopathogenesis, Risk Factors, Treatment: Literature Review
Published 2024-06-01“…It highlights the potential of CRISPR/Cas9, immunotherapies, and liquid biopsies in pioneering personalized CRC treatments and early detection. …”
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166
The use of maize haploidy inducers as a tool in agricultural plant biotechnology
Published 2022-11-01“…Thanks to this technology, it became possible to improve haploid inducers by introducing various CRISPR/cas constructs into the haploid inducer genome for editing any DNA site. …”
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167
Innovative Approaches to Suppressing Pseudomonas aeruginosa Growth and Virulence: Current Status and Future Directions
Published 2024-01-01“…Additionally, novel approaches, including RNA interference, CRISPR-Cas systems, and nanotechnology, show promise in preclinical studies. …”
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168
GGCX promotes Eurasian avian-like H1N1 swine influenza virus adaption to interspecies receptor binding
Published 2025-01-01“…In this study, we conduct a comprehensive genome-wide CRISPR/Cas9 knockout screen utilizing EA H1N1 SIV in porcine kidney cells. …”
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169
HP1 Promotes the Centromeric Localization of ATRX and Protects Cohesion by Interfering Wapl Activity in Mitosis
Published 2025-01-01“…Methods: The clustered regularly interspaced short palindromic repeats with CRISPR-associated protein 9 (CRISPR-Cas9) system and overexpression approaches were employed alongside immunofluorescence to investigate the mechanism of ATRX localization at the centromere. …”
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170
THERAPEUTIC GENE EDITING FOR HEMOGLOBINOPATHIES
Published 2024-08-01“…Initial studies of gene therapy for hemoglobinopathies involved the use of lentiviral vectors to add functional copies of the gene encoding b-globin in defective CD34 cells; more recently, gene editing techniques have been used involving either CRISPR-Cas9, transcription activation-like effector protein nuclease, zinc finger nuclease, and base editing to either induce fetal hemoglobin production at therapeutic levels or to genetically repair the underlying molecular defect causing the disease. …”
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171
Genomic Analysis Methods of Microorganisms
Published 2025-01-01“…Sequencing provides genetic data by identifying DNA sequences; Sanger sequencing is the basis for next-generation approaches. CRISPR modifies the genetic code and can correct mutations or control genes using Cas9. …”
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172
Advances in lignocellulosic feedstocks for bioenergy and bioproducts
Published 2025-02-01“…It also explores emerging biotechnologies such as CRISPR-based genome editing informed by machine learning, aimed at improving feedstock traits and reducing the environmental impact of fossil fuel dependence.…”
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173
Advancing the genetic engineering toolbox by combining AsCas12a knock-in mice with ultra-compact screening
Published 2025-01-01“…Our enAsCas12a mouse and accompanying crRNA libraries enhance genome engineering capabilities and complement current CRISPR technologies.…”
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174
A relay race of ESCRT-III paralogs drives cell division in a hyperthermophilic archaeon
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175
Biosécurité et surveillance épidémiologique
Published 2024-11-01“…Examples are the monitoring of sewer systems and the development of CRISPR tools for detection of biological agents. A new problem is that these tools can be used out of the research laboratories, because they are relatively cheap and easy to develop. …”
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176
A REVIEW ON RECENT ADVANCES IN ANIMAL BIOTECHNOLOGY
Published 2023-09-01“…Recent advancements encompass various domains, including genetic editing techniques like CRISPR-Cas9, which allow precise genetic modifications for improved animal health and product quality. …”
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177
A COMPREHENSIVE REVIEW ON-APPLICATION AND FUTURE PROSPECT OF CYTOPLASMIC MALE STERILITY
Published 2023-05-01“…From the identification of diverse CMS sources to the utilization of advanced genetic engineering techniques like CRISPR-Cas gene editing, the future of CMS in crop breeding appears promising. …”
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178
How data science and AI-based technologies impact genomics
Published 2023-01-01“…In this review, we focus on the application of data science and AI technology in three areas, including risk prediction and identification of causal single-nucleotide polymorphisms, EHR-based phenotyping and CRISPR guide RNA design. Additionally, we highlight a few emerging AI technologies, such as transfer learning and multi-view learning, which will or have started to benefit genomic studies.…”
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179
Genetic disruption of Blimp-1 drastically augments the antitumor efficacy of BCMA-targeting CAR T cells
Published 2025-02-01“…To improve long-term efficacy, we used CRISPR/CRISPR-associated protein 9-mediated gene editing to ablate the expression of the transcription factor Blimp-1. …”
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180
mGBP2 engages Galectin-9 for immunity against Toxoplasma gondii.
Published 2025-01-01“…Furthermore, we could prove the requirement of Gal9 for growth control of T. gondii by CRISPR/Cas9 mediated gene editing. These discoveries clearly indicate that Gal9 is a crucial factor for the mGBP2-coordinated cell-autonomous host defense mechanism against T. gondii.…”
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