Successful establishment of CRISPR-based genome-edited clonal cell populations from primary cells of buffalo, goats, and sheep by Priyanka Singh, Bosco Jose, Devika Gautam, Aseem Tara, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

Subjects: “…CRISPR…”
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Multi-gene precision editing tool using CRISPR-Cas12a/Cpf1 system in Ogataea polymorpha by Senqin Hou, Shibin Yang, Wenqin Bai

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CRISPR/Cas13a combined with reverse transcription and RPA for NoV GII.4 monitoring in water environments by Yiqiang Sun, Weiwei Zhang, Houyun Zhang, Feng Zhao, Laijin Su

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Protocol for functional screening of CFTR-targeted genetic therapies in patient-derived organoids using DETECTOR deep-learning-based analysis by Mattijs Bulcaen, Ronald B. Liu, Kasper Gryspeert, Sam Thierie, Anabela S. Ramalho, François Vermeulen, Xavier Casadevall I Solvas, Marianne S. Carlon

Subjects: “…CRISPR…”
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Enhanced silk production and pupal weight in Bombyx mori through CRISPR/Cas9-mediated circadian Clock gene disruption. by Daniel J Brady, Alessio Saviane, Matteo Battistolli, Irene Varponi, Federica Barca, Kunihiro Shiomi, Silvia Cappellozza, Federica Sandrelli

“…This study investigates the role of the circadian clock gene Clock in B. mori using CRISPR/Cas9-mediated mutagenesis to establish the ClkΔ29 knock-out mutant strain. …”
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Comparison of CRISPR-Cas13b RNA base editing approaches for USH2A-associated inherited retinal degeneration by Lewis E. Fry, Lauren Major, Ahmed Salman, Lucy A. McDermott, Jun Yang, Andrew J. King, Michelle E. McClements, Robert E. MacLaren

“…Abstract CRISPR-Cas13 systems have therapeutic promise for the precise correction of point mutations in RNA. …”
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استفاده از سیستم CRISPR/Cas9 برای دستکاری ژن SGR فستوکای بلند بر اساس بیان موقت by مصطفی خوشحال سرمست

Subjects: “…crispr/cas9…”
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CRISPR-Based Programmable Nucleic Acid-Binding Protein Technology Can Specifically Detect Fatal Tropical Disease-Causing Pathogens by Md. Rashidur Rahman, Toma Rani Majumder, Md. Aminul Islam Apu, Alok K. Paul, Afrina Afrose, Biplab Kumar Dash

“…It consists of CRISPR-associated proteins (Cas) capable of targeting virtually any specific RNA or DNA genome based on the guide RNA (gRNA) sequence. …”
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A universal and wide-range cytosine base editor via domain-inlaid and fidelity-optimized CRISPR-FrCas9 by Lan Hu, Jing Han, Hao-Da Wang, Zhou-Hua Cheng, Chang-Ce Lv, Dong-Feng Liu, Han-Qing Yu

“…Abstract CRISPR-based base editor (BE) offer diverse editing options for genetic engineering of microorganisms, but its application is limited by protospacer adjacent motif (PAM) sequences, context preference, editing window, and off-target effects. …”
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A CRISPR/Cas12a electrochemical biosensing to detect pig mtDNA D-loop for ensuring food authenticity by Muhammad Ihda H.L. Zein, Clianta Yudin Kharismasari, Ari Hardianto, Salma Nur Zakiyyah, Riezki Amalia, Mehmet Ozsoz, Mara Mirasoli, Irkham, Yeni Wahyuni Hartati

Subjects: “…CRISPR/Cas12a…”
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Dual inhibition of BET and EP300 has antitumor activity in undifferentiated pleomorphic sarcomas and synergizes with ferroptosis induction by Stéphanie Verbeke, Aurélien Bourdon, Mathilde Lafon, Vanessa Chaire, Bertolo Frederic, Amina Naït Eldjoudi, Marie-Alix Derieppe, Francis Giles, Antoine Italiano

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Constitutive expression of Cas9 and rapamycin-inducible Cre recombinase facilitates conditional genome editing in Plasmodium berghei by Samhita Das, Tanaya Unhale, Carine Marinach, Belsy del Carmen Valeriano Alegria, Camille Roux, Hélène Madry, Badreddine Mohand Oumoussa, Rogerio Amino, Shiroh Iwanaga, Sylvie Briquet, Olivier Silvie

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Effect of custom-designed transfection buffer on delivery of genome modification components into primary cells of buffalo, cattle, goats, and sheep by Shreya Malhotra, Priyanka Singh, Aseem Tara, Bosco Jose, Devika Gautam, Ram Prasad, Gaurav Tripathi, Sacchinandan De, Naresh L. Selokar

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Staphylococcus aureus nt5 gene mutation through CRISPR RNA-guided base editing weakens bacterial virulence and immune evasion by Xinpeng Liu, Lan Huang, Yang Ye, Haiyi Wang, Min Tang, Fuqiang He, Zijing Xia, Shi Deng, Peng Zhang, Ruiwu Dai, Shufang Liang

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Production of β-lactoglobulin (BLG) gene knock- out blastocyst stage embryos of Indian water buffalo using CRISPR and SCNT technology by Aseem Tara, Priyanka Singh, Devika Gautam, Gaurav Tripathi, Shreya Malhotra, Sacchinandan De, Manoj K. Singh, Naresh L. Selokar

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Detection of CRISPR‒Cas and type I R–M systems in Klebsiella pneumoniae of human and animal origins and their relationship to antibiotic resistance and virulence by Xue Li, Ling Wang, Jinghuan Lin, Yingjuan Gu, Zhihua Liu, Jing Hu

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Generation of IgM+ B cell-deficient Atlantic salmon (Salmo salar) by CRISPR/Cas9-mediated IgM knockout by Mari Raudstein, Ma. Michelle D. Peñaranda, Erik Kjærner-Semb, Søren Grove, H. Craig Morton, Rolf Brudvik Edvardsen

“…In this study, we used CRISPR/Cas9 technology to knock out the two IgM genes in Atlantic salmon. …”
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Topologically constrained DNA-mediated one-pot CRISPR assay for rapid detection of viral RNA with single nucleotide resolutionResearch in context by Yanan Li, Ru Xu, Fenglei Quan, Yonghua Wu, Yige Wu, Yongyuan Zhang, Yan Liang, Zhenzhong Zhang, Hua Gao, Ruijie Deng, Kaixiang Zhang, Jinghong Li

Subjects: “…CRISPR diagnostic…”
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CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris by Kaidi Chen, Gulikezi Maimaitirexiati, Qiannan Zhang, Yi Li, Xiangjian Liu, Hongting Tang, Xiang Gao, Bo Wang, Tao Yu, Shuyuan Guo

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A CRISPR-Cas12a-based universal rapid scrub typhus diagnostic method targeting 16S rRNA of Orientia tsutsugamushi. by Bum Ju Park, Sang Taek Heo, Misun Kim, Jeong Rae Yoo, Eun Jin Bae, Su Yeon Kang, Sunghoon Park, Kyeo Re Han, Keun Hwa Lee, Jae Myun Lee, Hyeyoung Lee, Yoon-Jae Song

“…Here, we developed a CRISPR-Cas12a-based diagnostic method targeting the bacterial 16S rRNA to detect O. tsutsugamushi infection of all known genotypes. …”
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