Synergistic strategies for glioblastoma treatment: CRISPR-based multigene editing combined with immune checkpoint blockade by Xiaolin Liu, Xiao Liu, Xiaonan Luo, Maorong Zhu, Nannan Liu, Juan Li, Qi Zhang, Cheng Zou, Yuxin Wu, Zhengcong Cao, Shuangxin Ma, Weizhong Wang, Guangzhao Yang, Jintao Gu, Wei Liu, Meng Li, Anan Yin, Yalong He, Wei Lin

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Generation and propagation of high fecundity gene edited fine wool sheep by CRISPR/Cas9 by Xuemei Zhang, Meiyu Qiu, Bing Han, Li Liao, Xinrong Peng, Jiapeng Lin, Ning Zhang, Lati Hai, Long Liang, Yila Ma, Wenrong Li, Mingjun Liu

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An Efficient Method for Immortalizing Mouse Embryonic Fibroblasts by CRISPR-mediated Deletion of the Tp53 Gene by Srisathya Srinivasan, Hsin-Yi Ho

“…By using electroporation to deliver CRISPR constructs that target the Tp53 gene, the method reliably generates immortalized MEFs (iMEFs) within three weeks. …”
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PathoGD: an integrative genomics approach to primer and guide RNA design for CRISPR-based diagnostics by Soo Jen Low, Matthew O’Neill, William J. Kerry, Natasha Wild, Marcelina Krysiak, Yi Nong, Francesca Azzato, Eileen Hor, Lewis Williams, George Taiaroa, Eike Steinig, Shivani Pasricha, Deborah A. Williamson

“…Abstract Critical to the success of CRISPR-based diagnostic assays is the selection of a diagnostic target highly specific to the organism of interest, a process often requiring iterative cycles of manual selection, optimisation, and redesign. …”
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Разработка подходов скрининга высокоспецифичных бактериофагов на основе биоинформационного анализа структур CRISPR-Cas by Л.А. Степаненко*, Ю.П. Джиоев*, В.И. Злобин*, А.Ю. Борисенко*, В.П. Саловарова **, Н.А. Арефьева **, И.Ж. Семинский *, И.В. Мало

CRISPR/Cas9-mediated genomic insertion of functional genes into Lactiplantibacillus plantarum WCFS1 by Kamilla Wiull, Lisa K. Haugen, Vincent G. H. Eijsink, Geir Mathiesen

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CRISPR/Cas9-Based Protocol for Precise Genome Editing in Induced Pluripotent Stem Cells by Avinash Singh, Swathy Babu, Marcus Phan, Shauna Yuan

“…The advent of clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9-based genome editing has marked a significant advancement in genetic engineering technology. …”
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RNA-Targeting CRISPR/CasRx system relieves disease symptoms in Huntington’s disease models by Yingqi Lin, Caijuan Li, Yizhi Chen, Jiale Gao, Jiawei Li, Chunhui Huang, Zhaoming Liu, Wei Wang, Xiao Zheng, Xichen Song, Jianhao Wu, Jiaxi Wu, Oscar Junhong Luo, Zhuchi Tu, Shihua Li, Xiao-Jiang Li, Liangxue Lai, Sen Yan

Subjects: “…CRISPR/CasRx…”
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Robust genome editing activity and the applications of enhanced miniature CRISPR-Cas12f1 by Soo-Ji Park, Sungjin Ju, Won Jun Jung, Tae Yeong Jeong, Da Eun Yoon, Jang Hyeon Lee, Jiyun Yang, Hojin Lee, Jungmin Choi, Hyeon Soo Kim, Kyoungmi Kim

“…A key factor facilitating efficient gene editing is effective CRISPR delivery into cells, which is known to be associated with the size of the CRISPR system. …”
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Development of a genetically tailored implantation hepatocellular carcinoma model in Oncopigs by somatic cell CRISPR editing by Lobna Elkhadragy, Maximillian J. Carlino, Luke R. Jordan, Thomas Pennix, Nahed Ismail, Grace Guzman, Jonathan P. Samuelson, Lawrence B. Schook, Kyle M. Schachtschneider, Ron C. Gaba

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Prevalent integration of genomic repetitive and regulatory elements and donor sequences at CRISPR-Cas9-induced breaks by Chongwei Bi, Baolei Yuan, Yingzi Zhang, Mengge Wang, Yeteng Tian, Mo Li

“…Abstract CRISPR-Cas9 genome editing has been extensively applied in both academia and clinical settings, but its genotoxic risks, including large insertions (LgIns), remain poorly studied due to methodological limitations. …”
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Creation of body color mutants by CRISPR/Cas9 gene editing in largemouth bass, Micropterus salmoides by Lina Sun, Tian Gao, Zhiqiang Li, Xiaolong Yang, Zuliang Qin, Mingxiao Ye, Ying Li, Feilong Wang, Deshou Wang

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Rapid detection of FAdV-4 by one-tube RPA-CRISPR/Cas12a assay by Lei Ma, Xueping Wang, Mingliang Zhang, Mengjie Zhu

“…Additionally, the agreement rate between the results of RPA-CRISPR/Cas12a and qPCR for detecting clinical samples is as high as 97.5%.DiscussionTherefore, the RPA-CRISPR/Cas12a assay presents a promising alternative for the simple, sensitive, and specific identification of FAdV-4.…”
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New frontiers in CRISPR: Addressing antimicrobial resistance with Cas9, Cas12, Cas13, and Cas14 by Ahmed S.A. Ali Agha, Ali Al-Samydai, Talal Aburjai

Subjects: “…CRISPR-Cas…”
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A hypomorphic mutation in the mouse Csn1s1 gene generated by CRISPR/Cas9 pronuclear microinjection by A. V. Smirnov, T. A. Shnaider, A. N. Korablev, A. M. Yunusova, I. A. Serova, N. R. Battulin

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Improvement of the knock-in effciency in the genome of human induced pluripotent stem cells using the CRISPR/Cas9 system by М. М. Gridina

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Integrating malaria vaccine and CRISPR/Cas9 gene drive: a comprehensive strategy for accelerated malaria eradication by Israel Charles Abraham, John Ehi Aboje, Bonaventure Michael Ukoaka, Kehinde Tom-Ayegunle, Maryam Amjad, Anas Abdulkader, Chinonyelum Emmanuel Agbo, Oluwatosin Ayokunle Akinruli, Taiwo Rebecca Akisanmi, Emmanuel Oyedeji Oyetola, Gbolahan Olatunji, Emmanuel Kokori, Nicholas Aderinto

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Cellular Reprogramming, Genome Editing, and Alternative CRISPR Cas9 Technologies for Precise Gene Therapy of Duchenne Muscular Dystrophy by Peter Gee, Huaigeng Xu, Akitsu Hotta

“…In this review, we highlight recent advances made using the CRISPR Cas9 system to correct genetic mutations and discuss how emerging CRISPR technologies and iPSCs in a combined platform can play a role in bringing a therapy for DMD closer to the clinic.…”
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CRISPR-based epigenetic editing of Gad1 improves synaptic inhibition and cognitive behavior in a Tauopathy mouse model by Lei Wan, Ping Zhong, Pei Li, Yong Ren, Wei Wang, Mingjun Yu, Henry Y. Feng, Zhen Yan

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Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean by Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith

“…Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. …”
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