ParSite is a multicolor DNA labeling system that allows for simultaneous imaging of triple genomic loci in living cells. by Xiaohui He, Yadong Sun, Hanhui Ma

“…A pair of genomic loci labeled by ParSite could be colocalized with p53-binding protein 1 (53BP1) in response to CRISPR/Cas9-mediated double-strand breaks (DSBs). …”
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The BnaBPs gene regulates flowering time and leaf angle in Brassica napus by Jiang Yu, Yi‐Xuan Xue, Rehman Sarwar, Shi‐Hao Wei, Rui Geng, Yan‐Feng Zhang, Jian‐Xin Mu, Xiao‐Li Tan

“…The protein sequence analysis showed two proteins containing conserved domains KNOX I, KNOX II, ELK, and HOX of the KONX protein family. The CRISPR/Cas9 knockout lines exhibited early budding and flowering time, coupled with floral organ abscission earlier and a larger leaf angle. …”
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Gene Editing in Pluripotent Stem Cells and Their Derived Organoids by Hang Zhou, Yun Wang, Li-Ping Liu, Yu-Mei Li, Yun-Wen Zheng

“…Gene-editing technologies, from large-scale nucleic acid endonucleases to CRISPR, have ignited a global research and development boom with significant implications in regenerative medicine. …”
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Live Visualization of Calcified Bones in Zebrafish and Medaka Larvae and Juveniles Using Calcein and Alizarin Red S by Rina Koita, Sae Oikawa, Taisei Tani, Masaru Matsuda, Akinori Kawamura

“…Zebrafish and medaka are valuable model vertebrates for genetic studies. The advent of CRISPR-Cas9 technology has greatly enhanced our capability to produce specific gene mutants in zebrafish and medaka. …”
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Casein Kinases 2-dependent phosphorylation of the placental ligand VAR2CSA regulates Plasmodium falciparum-infected erythrocytes cytoadhesion. by Dominique Dorin-Semblat, Jean-Philippe Semblat, Romain Hamelin, Anand Srivastava, Marilou Tetard, Graziella Matesic, Christian Doerig, Benoit Gamain

“…Mass spectrometry analysis of recombinant VAR2CSA phosphorylated by recombinant Human and P. falciparum CK2α combined with site-directed mutagenesis led to the identification of residue S1068 in VAR2CSA, which is phosphorylated by both enzymes and is associated with CSA binding. Furthermore, using CRISPR/Cas9 we generated a parasite line in which phosphoresidue S1068 was changed to alanine. …”
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A strategy for controlling Hypervirulent Klebsiella pneumoniae: inhibition of ClpV expression by Wenke Liu, Dan Wang, Qiangxing He, Shiwen Cao, Jiaxin Cao, Huajie Zhao, Junwei Cui, Fan Yang

“…In the present study, the clpV gene deletion strain of hvKP (ΔclpV-hvKP) was constructed using CRISPR-Cas9 technology, and the biological characteristics of ΔclpV-hvKP were investigated to explore the new targets for controlling this pathogen. …”
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Identification of mitochondrial carrier homolog 2 as an important therapeutic target of castration-resistant prostate cancer by Yankui Liu, Anjie Chen, Yufan Wu, Jiang Ni, Rong Wang, Yong Mao, Ning Sun, Yuanyuan Mi

“…Using genetic silencing via shRNA and knockout (KO) through the CRISPR-sgRNA approach, we showed that the depletion of MTCH2 impaired mitochondrial function, resulting in a reduced oxygen consumption rate, diminished complex I activity, and decreased ATP levels, mitochondrial depolarization, and increased reactive oxygen species production in primary CRPC cells. …”
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An insight into the genome-wide analysis of bacterial defense mechanisms in a uropathogenic Morganella morganii isolate from Bangladesh. by Syed Muktadir Al Sium, Barna Goswami, Sanjana Fatema Chowdhury, Showti Raheel Naser, Mihir Kanti Sarkar, Md Jobaid Faruq, Md Ahashan Habib, Shahina Akter, Tanjina Akhtar Banu, Md Murshed Hasan Sarkar, Md Salim Khan

“…The genome contained mobile genetic components and defense mechanisms, such as restriction modification and CRISPR-Cas systems, indicating the bacterium's ability to defend itself against viral attacks. …”
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Development of cell lines with increased susceptibility to diverse adeno-associated viral vectors to enable in vitro potency assays by James Zengel, Emma S. Esterman, Anitha Ponnuswami, Nicholas R. Wall, Jan E. Carette

“…Here, we enhance the efficiency of in vitro AAV transduction by overexpressing the AAV receptor (AAVR/KIAA0319L), preventing transcriptional silencing by the HUSH complex, and using CRISPR activation (CRISPRa) to drive transgene expression. …”
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Advances in regenerative medicine-based approaches for skin regeneration and rejuvenation by Nathalia Silva Dutra Alves, Gustavo Roncoli Reigado, Mayara Santos, Izabela Daniel Sardinha Caldeira, Henrique dos Santos Hernandes, Bruna Leticia Freitas-Marchi, Elina Zhivov, Felipe Santiago Chambergo, Viviane Abreu Nunes

“…This review examines core technologies including stem cell therapy, bioengineered skin substitutes, platelet-rich plasma (PRP), exosome-based therapies, and gene editing techniques like CRISPR. These methods hold promise for treating a range of conditions, from chronic wounds and burns to age-related skin changes and genetic disorders. …”
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A Foxf1-Wnt-Nr2f1 cascade promotes atrial cardiomyocyte differentiation in zebrafish. by Ugo Coppola, Bitan Saha, Jennifer Kenney, Joshua S Waxman

“…Functionally, we find that Wnt signaling acts downstream of Foxf1 to promote expression of the 3'reg1 reporter within ACs and, importantly, both Foxf1 and Wnt signaling require Nr2f1a to promote a surplus of differentiated ACs. CRISPR-mediated deletion of the endogenous 3'reg1 abrogates the ability of Foxf1 and Wnt signaling to produce surplus ACs in zebrafish embryos. …”
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The role of microRNAs in learning and long-term memory by L. N. Grinkevich

“…In addition, analysis is provided of the current literature related to the prospects of improving cognitive processes by influencing microRNA biogenesis via the use of CRISPR/Cas9 technologies and active mental and physical exercises.…”
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Light quality‐dependent roles of REVEILLE proteins in the circadian system by Cassandra L. Hughes, Yuyan An, Julin N. Maloof, Stacey L. Harmer

“…To clarify the function of the REVEILLE 4, REVEILLE 6, and REVEILLE 8 transcriptional activators, we characterized the growth and clock phenotypes of CRISPR‐Cas9‐generated single, double, and triple rve mutants. …”
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DNA Ligase IV Prevents Replication Fork Stalling and Promotes Cellular Proliferation in Triple Negative Breast Cancer by Rashmi R. Joshi, Sk Imran Ali, Amanda K. Ashley

“…In addition, Lig4 is an attractive clinical target for directing CRISPR/Cas9-mediated repair towards homology-directed repair and away from NHEJ, thus understanding of how diminishing Lig4 impacts cell biology is critical.…”
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dotears: Scalable and consistent directed acyclic graph estimation using observational and interventional data by Albert Xue, Jingyou Rao, Sriram Sankararaman, Harold Pimentel

“…Summary: New assays such as Perturb-seq link parallel CRISPR interventions to transcriptomic readouts, providing insight into gene regulatory networks. …”
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A versatile site-directed gene trap strategy to manipulate gene activity and control gene expression in Caenorhabditis elegans. by Haania Khan, Xinyu Huang, Vishnu Raj, Han Wang

“…We designed a cGAL gene trap cassette and used CRISPR/Cas9 to insert it into the target gene, creating a bicistronic operon that simultaneously expresses a truncated endogenous protein and the cGAL driver in the cells expressing the target gene. …”
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Increased distribution of carbon metabolic flux during de novo cytidine biosynthesis via attenuation of the acetic acid metabolism pathway in Escherichia coli by Tong Ye, Wei Ding, Zhengxu An, Haojie Zhang, Xiaobo Wei, Junnan Xu, Huiyan Liu, Haitian Fang

“…To mitigate carbon source loss, the CRISPR-Cas9 gene-editing technique was employed to knock out the poxB and pta genes in E. coli, generating the engineered strains K12ΔpoxB and K12ΔpoxBΔpta. …”
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Glycosylation of ceramide synthase 6 is required for its activity by Alexandra J. Straus, Grace Mavodza, Can E. Senkal

“…We used a robust HCT116 CRISPR-Cas9 CerS6 KO with reintroduction of either WT CerS6 or a mutant CerS6 with a point mutation at asparagine-18 to an alanine (N18A) which abrogated glycosylation at that residue. …”
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Comparative Genomics of the First and Complete Genome of “Actinobacillus porcitonsillarum” Supports the Novel Species Hypothesis by Valentina Donà, Vincent Perreten

“…The four strains showed distinct and shared transposable elements, CRISPR/Cas systems, and integrated prophages. Genome comparisons based on average nucleotide identity and in silico DNA-DNA hybridization confirmed the close relationship among strains belonging to the A. minor/“porcitonsillarum” complex compared to other Actinobacillus spp., but also suggested that 9953L55 and 202 belong to the same novel species closely related to A. minor, namely, “A. porcitonsillarum.” …”
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<i>Macrostomum lignano</i> as a model to study the genetics and genomics of parasitic flatworms by K. V. Ustyantsev, V. Yu. Vavilova, A. G. Blinov, E. V. Berezikov

“…All this makes M. lignano amenable to the most modern approaches of forward and reverse genetics, such as transposon insertional mutagenesis and methods of targeted genome editing by the CRISPR/Cas9 system. Due to the availability of an increasing number of genome and transcriptome assemblies of different parasitic flatworm species, new knowledge generated by studying M. lignano can be easily translated to parasitic f latworms with the help of modern bioinformatic methods of comparative genomics and transcriptomics. …”
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