Aging impairs CD8 T cell responses in adoptive T-cell therapy against solid tumors by Gulfiya Kadyrzhanova, Miho Tamai, Shukla Sarkar, Rajkumar Singh Kalra, Hiroki Ishikawa

“…Aged CD8 TCR-T cells are unable to accumulate efficiently in tumors and have higher tendency to become terminally exhausted T cells with lower expression of endothelial PAS domain-containing protein 1 (Epas1) compared to young cells. Crispr-mediated ablation of Epas1 promotes terminal exhaustion of young CD8 T cells in tumors, diminishing their anti-tumor activity in young mice. …”
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Harnessing artificial intelligence for enhanced nanoparticle design in precision oncology by Mujibullah Sheikh, Pranita S. Jirvankar

“…AI is presented as capable of creating synergies with bioengineering methods like CRISPR or gene therapy and able to show potential directions for enhancing the efficiency of nanoparticle-based cancer therapies. …”
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Novel Approaches to Diagnose COVID-19 by Solaf Jawhar Ali

“…More recently, a new CRISPR-based DETECTR assay has been developed to detect COVID-19. …”
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Nanomédicaments pour le traitement de maladies graves et recherches duales by Couvreur, Patrick

“…This review aims to describe and discuss the characteristics and functionalities of the different generations of nanocarriers, from their inception to the present day, discussing the prospects they offer for the production of therapeutic proteins, for facilitating gene editing (CRISPR/Cas9), and for enabling immune checkpoint blockade in oncology. …”
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Are You My Host? An Overview of Methods Used to Link Bacteriophages with Hosts by Paul Hyman

“…., proximity ligation, polonies, viral tagging); and analysis of genomes and metagenomes (e.g., CRISPR spacer analysis, metagenomic co-occurrence). …”
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Stage-specific expression and divergent functions of two insulinase-like proteases associated with host infectivity in Cryptosporidium. by Yue Huang, Shifeng Pei, Xin Lv, Fuxian Yang, Xiaoqing Gong, Na Li, Yaqiong Guo, Yaoyu Feng, Lihua Xiao

“…<h4>Methodology/principal findings</h4>In this study, we investigated the expression and biological function of the INS-19 and INS-20 of C. parvum. CRISPR/Cas9 was used to endogenously tag both genes with the hemagglutinin epitope. …”
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Synthetic Gene Drives in Plants: Development Strategies, Potential Applications, and Ethical Considerations by Yawen Liu, Bingke Jiao, Wenfeng Qian

“…In this perspective, we introduce the principles underlying our development of a plant synthetic toxin‐antidote drive, CAIN (CRISPR‐Assisted Inheritance utilising NPG1). We evaluate the potential of gene drives to alter the genetics of weed populations or suppress invasive plant populations. …”
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Human cytomegalovirus RNA2.7 inhibits ferroptosis by upregulating ferritin and GSH via promoting ZNF395 degradation. by Mingyi Xu, Shan Ruan, Jingxuan Sun, Jianming Li, Dan Chen, Yanping Ma, Ying Qi, Zhongyang Liu, Qiang Ruan, Yujing Huang

“…The results further showed that RNA2.7 inhibited ferroptosis via enhancing Ferritin Heavy Chain 1 (FTH1) and Solute Carrier Family 7 Member 11 (SLC7A11) expression in Erastin treated cells without involving other viral components. Pooled Genome-wide CRISPR screening revealed zinc finger protein 395 (ZNF395) as a new regulator repressing the expression of FTH1 and SLC7A11. …”
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The role of epigenetics in plant pathogens interactions under the changing environments: A systematic review by Wenhao Wu, Guoqiang Fan

“…Advances in high-throughput sequencing and CRISPR-based tools are shedding light on these complex interactions, offering opportunities to engineer crops with enhanced disease resistance. …”
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The Proviral Reservoirs of Human Immunodeficiency Virus (HIV) Infection by Andrey I. Murzin, Kirill A. Elfimov, Natalia M. Gashnikova

“…Uncovering the causes of this phenomenon will bring humanity closer to curing HIV infection, potential approaches to which include stem cell transplantation, clustered regularly interspaced short palindromic repeats (CRISPR)/cas9, “Shock and kill”, “Block and lock”, and the application of broad-spectrum neutralizing antibodies (bNAbs).…”
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SLC35A2 gene product modulates paramyxovirus fusion events during infection. by Yanling Yang, Yuchen Wang, Danielle E Campbell, Heng-Wei Lee, Wandy Beatty, Leran Wang, Megan Baldridge, Carolina B López

“…Using a recombinant SeV expressing destabilized eGFP (rSeVCdseGFP) in a loss-of-function CRISPR screen, we identified the CMP-sialic acid transporter (CST) gene SLC35A1 and the UDP-galactose transporter (UGT) gene SLC35A2 as essential for paramyxovirus infection. …”
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Exploring Novel Non-Operative Treatments for Osteoarthritis: An Examination of Innovative Therapies by Piotr Robert Bakuła, Kamila Jałocha, Joanna Zygadło

“…Cutting-edge technologies such as RNA interference, CRISPR/Cas9, and advanced regenerative medicine are also explored for their potential to revolutionize OA management. …”
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Single-cell RNA sequencing and AlphaFold 3 insights into cytokine signaling and its role in uveal melanoma by Hongyan Sun, Cunzi Li, Zuhui Pu, Zuhui Pu, Ying Lu, Ying Lu, Zijing Wu, Zijing Wu, Lan Zhou, Lan Zhou, Hongzhan Lin, Yumo Wang, Tao Zi, Lisha Mou, Lisha Mou, Ming-ming Yang

“…T-cell populations were analyzed using pseudotime trajectory mapping and interaction network visualization. CRISPR-Cas9 screening analysis was conducted to identify hub genes and cytokine pathways that may serve as therapeutic targets. …”
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Loss of MEF2C function by enhancer mutation leads to neuronal mitochondria dysfunction and motor deficits in mice by Ali Yousefian-Jazi, Suhyun Kim, Jiyeon Chu, Seung-Hye Choi, Phuong Thi Thanh Nguyen, Uiyeol Park, Min-gyeong Kim, Hongik Hwang, Kyungeun Lee, Yeyun Kim, Seung Jae Hyeon, Hyewhon Rhim, Hannah L. Ryu, Grewo Lim, Thor D. Stein, Kayeong Lim, Hoon Ryu, Junghee Lee

“…MEF2C localizes and binds to the mitochondria DNA, and directly modulates mitochondria-encoded gene expression. CRISPR/Cas-9-induced mutation of the MEF2C enhancer decreases expression of mitochondria-encoded genes. …”
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Knock-In Mice with Myo3a Y137C Mutation Displayed Progressive Hearing Loss and Hair Cell Degeneration in the Inner Ear by Peipei Li, Zongzhuang Wen, Guangkai Zhang, Aizhen Zhang, Xiaolong Fu, Jiangang Gao

“…To investigate the mechanism of DFNB30-type deafness, we established a mouse model of Myo3a kinase domain Y137C mutation by using CRISPR/Cas9 system. No difference in hearing between 2-month-old Myo3a mutant mice and wild-type mice was observed. …”
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Nuclear-Targeting Delivery of CRISPRa System for Upregulation of β-Defensin against Virus Infection by Dexamethasone and Phenylalanine Dual-Modified Dendrimer by Mingxiang Zuo, Xiaoxia Li, Shuang Liu, Bin Chen, Du Cheng

“…In vivo experiments demonstrated that the DET- and Phe-modified dendrimer effectively delivered plasmid DNA encoding dCas9 protein into the airway epithelium, inducing β-defensin expression. Delivery of the CRISPR activation system by a dendrimer modified with DET and Phe was a promising approach against viral disease.…”
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Developmental defects in ectodermal appendages caused by missense mutation in edaradd gene in the nfr mangrove killifish kryptolebias marmoratus by Hussein A. Saud, Paul A. O’Neill, Brian C. Ring, Tetsuhiro Kudoh

“…To confirm the crucial role of edaradd during fin development, CRISPR RNAs were designed to suppress the gene in another killifish species, Arabian killifish. …”
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Creation of a novel zebrafish model with low DHA status to study the role of maternal nutrition during neurodevelopment by Katherine M. Ranard, Bruce Appel

“…Here, we create a novel DHA-deficient zebrafish model by 1) disrupting elovl2, a key gene in the DHA biosynthesis pathway, via CRISPR/Cas9 genome editing, and 2) feeding mothers a DHA-deficient diet. …”
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Recent Advances in Antimicrobial Resistance: Insights from <i>Escherichia coli</i> as a Model Organism by Zhaoyang Zhang, Minliang Wei, Bin Jia, Yingjin Yuan

“…Additionally, we highlight strategies to mitigate the spread of AMR, such as dynamic resistance monitoring, innovative therapies like phage therapy and CRISPR-Cas technology, and tighter regulation of antibiotic use in animal production systems. …”
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Identification of novel genes responsible for a pollen killer present in local natural populations of Arabidopsis thaliana. by Anthony Ricou, Matthieu Simon, Rémi Duflos, Marianne Azzopardi, Fabrice Roux, Françoise Budar, Christine Camilleri

“…In this study, we identified the two other genes of the pollen killer by using CRISPR-Cas9 induced mutants. These two genes are necessary for the killer activity that we demonstrated to be specific to pollen. …”
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