Protocol to generate PDMS topographical patterns for hiPSC-derived or rat primary neuronal cultures by Anna-Christina Haeb, Mireia Olives-Verger, Jordi Soriano

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Protocol for whole-tissue immunolabeling, optical clearing, and lightsheet imaging of c-Fos protein expression in unsectioned mouse brains by Koukou Fu, Jing Yang

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Protocols for translocation processes of flexible polymers through a pore using LAMMPS by Vrinda Garg, Rejoy Mathew, Arindam Chatterjee, Surya K. Ghosh

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Protocol for in vivo assessment of glucose control and insulin secretion and sensitivity in the pig by Yasmin Eckstein, Barbara Kessler, Arne Hinrichs, Istvan Novak, Anne von Thaden, Timo Lorenzen, Birgit Rathkolb, Armin Scholz, Andreas Blutke, Sietse-Jan Koopmans, Martin Hrabĕ de Angelis, Berit Østergaard Christoffersen, Eckhard Wolf, Simone Renner

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Protocol for 3D bioprinting of a 3D in vitro model of neuroblastoma by Mehdi Salar Amoli, Sarah Rezapourdamanab, Linqi Jin, Melissa A. Cadena, Kaveeta Kaw, Vani Sridhar, Mostafa Meselhe, Sarah Krikor, Morteza Mahmoudi, Liqun Ning, Jenny Shim, Martin L. Tomov, Kelly C. Goldsmith, Vahid Serpooshan

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Protocol for the decellularization of human intestinal tissue from patients with inflammatory bowel disease by Sinan Lin, Gail West, Jie Wang, Ren Mao, Ankita Prasad, Suhanti Banerjee, Pranab K. Mukherjee, Florian Rieder

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Protocol for decellularizing and delipidizing human pancreatic tissue into native hydrogel by Connie S. Chamberlain, Daniel M. Tremmel, Ayesha Palwasha Khan, Ellen Abad Santos, Deep Kapadia, Caterra Leavens, Katherine M. Gorski, Rawan Hamadeh, Austin K. Feeney, Jon Odorico, Sara D. Sackett

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Protocol for minicircle production for gene therapy without subsequent cleanup steps by Jonathan Do, Gautam Verma, Yasmin Maurice, Muniba Abdumanobova, Zicheng Deng, Tanya V. Kalin, Vladimir V. Kalinichenko

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Protocol for assisting frequency band definition and decoding neural dynamics using hierarchical clustering and multivariate pattern analysis by Chengpeng Li, Isao Hasegawa, Hisashi Tanigawa

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Protocol for recombinant rabies virus titration by quantitative PCR by He Zhang, Xueping Gao, Xiangyu Ge, Xiao Wang, Minghui Song, Xia Zhang, Lei Jin

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Protocol to assess the impact of sperm RNA on mouse preimplantation embryos using microinjection by Grace S. Lee, Natalie A. Trigg, Colin C. Conine

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Protocols to co-culture human primary lung cells in the simple-flow device by Cinta Iriondo, Sem Koornneef, Kari-Pekka Skarp, Marjon Buscop-van Kempen, Anne Boerema-de Munck, Robbert J. Rottier

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Protocol for fabricating a reusable plate-well insert with a 3D-printed mounter and hydrogel scaffolds for 3D cell culture and functional assays by Saloua Saghir, Jana Hlinkova, Athina Samara, Giuseppe Schiavone

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Protocol for measuring force in skinned diaphragm muscle fibers of myopathic SEPN1 knockout mice following chronic tauroursodeoxycholic acid treatment by Leonardo Nogara, Serena Germani, Cosimo De Napoli, Bert Blaauw, Ester Zito

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Protocol for the recombinant expression and purification of the LSAM domain of human legumain in E. coli by Sven O. Dahms, Alexander C. Wieland, Hans Brandstetter, Elfriede Dall

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Protocol for generation of transmitochondrial cybrids under pyruvate/uridine-supplemented conditions using a microfluidic device by Ken-Ichi Wada, Kazuo Hosokawa, Yoshihiro Ito, Mizuo Maeda, Yui Harada, Yoshikazu Yonemitsu

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Protocol for assessment of CRISPR base editors and their components in Escherichia coli by Rahul Mahadev Shelake, Jae-Yean Kim

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Protocol for fabricating elastomeric stencils for patterned stem cell differentiation by Stefanie Lehr, Jack Merrin, Monika Kulig, Thomas George Minchington, Anna Kicheva

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Protocol for producing brain-derived neurotrophic factor and neurotrophin-4 in their pro and active form in Escherichia coli by Christoph Holzner, Katharina Böttinger, Christian G. Huber, Elfriede Dall, Hans Brandstetter

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Protocol for end-design-free rebooting of terminally redundant Pseudomonas phages in clinical isolates of Pseudomonas aeruginosa by Daigo Yokoyama, Nana Kimura, Haruka Yamamoto, Yoshiaki Sakata, Jumpei Fujiki, Hidetomo Iwano

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