Harnessing Hazara Virus as a Surrogate for Crimean–Congo Hemorrhagic Fever Virus Enables Inactivation Studies at a Low Biosafety Level by Judith Olejnik, Kristina Meier, Jarod N. Herrera, Daniel J. DeStasio, Dylan J. Deeney, Elizabeth Y. Flores, Mitchell R. White, Adam J. Hume, Elke Mühlberger

“…Research on highly pathogenic biosafety level 4 (BSL-4) viruses that are classified as Select Agents involves transferring inactivated materials to lower containment levels for further analysis. …”
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Biosafety Aspects of Hybridoma Technology: Nature of Risks and Approaches to Their Management by Kateryna Shevchuk, Anastasia Baranovska, Andrii Chernetskyi, Nataliia Shchotkina, Alexander Besarab

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Enhancing safety with an AI-empowered assessment and monitoring system for BSL-3 facilities by Yi-Ling Fan, Ching-Han Hsu, Ju-Yu Wu, Ying-Ying Tsai, Wei J. Chen, Min-Shi Lee, Fang-Rong Hsu, Lun-De Liao

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Perceptions of psychological safety in high-containment laboratories: mixed method survey of community members and industry experts by Moana Sargent, Ashley Sylvara, Leah Klos, Jacob Gallagher, Kutina Cabrera, Molly Goff, Jesica Luna, Alfonso Clavijo, Jin Lee

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Inoculation route-dependent Lassa virus dissemination and shedding dynamics in the natural reservoir – Mastomys natalensis by D. M. Wozniak, S. A. Riesle-Sbarbaro, N. Kirchoff, K. Hansen-Kant, A. Wahlbrink, A. Stern, A. Lander, K. Hartmann, S. Krasemann, A. Kurth, J. Prescott

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Serological evidence of West Nile Virus infection among humans in the southern Provinces of Morocco by Hicham El Rhaffouli, Idriss Lahlou-Amine, Chafiqua Loutfi, Abdellilah Laraqui, Tahar Bajjou, Ouafaa Fassi-Fihri, Mehdi El Harrak

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LncRNA SNHG16 Inhibits Intracellular M. tuberculosis Growth Involving Cathelicidin Pathway, Autophagy, and Effector Cytokines Production by Guixian Huang, Xiaocui Wu, Xuejiao Ji, Ying Peng, Juechu Wang, Xia Cai, Yihui Wang, Enzhuo Yang, Liying Zhu, Yuan Wu, Qin Sun, Ling Shen, Wei Sha, Hongbo Shen, Feifei Wang

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Corrigendum to “Middle East respiratory syndrome coronavirus—a 10-year (2012-2022) global analysis of human and camel infections, genomic sequences, lineages, and geographical orig... by Esam I. Azhar, Thirumalaisamy P. Velavan, Ikrormi Rungsung, Tieble Traore, David S. Hui, Brian McCloskey, Sherif A. El-Kafrawy, Alimuddin Zumla

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A Large-Scale Multispecialty Evaluation of Web-Based Simulation in Medical Microbiology Laboratory Education: Randomized Controlled Trial by Lei Xu, Xichuan Deng, Tingting Chen, Nan Lu, Yuran Wang, Jia Liu, Yanan Guo, Zeng Tu, Yuxin Nie, Yeganeh Hosseini, Yonglin He

“…Our virtual system focused on biosafety level 2 pathogenic cocci identification with dynamic specimen generation. …”
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Preparation for the next pandemic: challenges in strengthening surveillance by Kelvin Hei-Yeung Chiu, Siddharth Sridhar, Kwok-Yung Yuen

“…Furthermore, sufficient regulation of high biosafety level laboratories, and stockpiling of broad spectrum antiviral drugs, vaccines, and personal protective equipment are indicated for pandemic preparedness.…”
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Evaluating Neutralizing Antibodies in Hantavirus-Infected Patients Using Authentic Virus and Recombinant Vesicular Stomatitis Virus Systems by Punya Shrivastava-Ranjan, Jamie A. Kelly, Laura K. McMullan, Deborah Cannon, Laura Morgan, Payel Chatterjee, Shilpi Jain, Joel M. Montgomery, Mike Flint, César G. Albariño, Christina F. Spiropoulou

“…In this study, we developed a recombinant vesicular stomatitis virus (VSV)-based surrogate system expressing SNV and ANDV glycoproteins (GPCs), enabling neutralization studies under biosafety level 2 conditions. The neutralization titers obtained with the VSV-based system closely matched the findings from authentic hantavirus assays performed under biosafety level 3 conditions, confirming its potential as a useful tool for determining immune responses and advancing hantavirus research.…”
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Penicillin-Susceptible, Oxidase-Negative, Nonhemolytic, Nonmotile Bacillus megaterium in Disguise of Bacillus anthracis by Shih Keng Loong, Boon Teong Teoh, Jefree Johari, Chee Sieng Khor, Juraina Abd-Jamil, Siti Sarah Nor’e, Nur Izyan Samsudin, Noor Syahida Azizan, Che Norainon Yaacob, Asma Anati CheMatSeri, Nur Hidayana Mahfodz, Sazaly AbuBakar

“…B. megaterium was identified as the causative agent and the organism was subsequently treated as a Biosafety Level 2 pathogen.…”
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Nipah pseudovirus system enables evaluation of vaccines in vitro and in vivo using non-BSL-4 facilities by Jianhui Nie, Lin Liu, Qing Wang, Ruifeng Chen, Tingting Ning, Qiang Liu, Weijin Huang, Youchun Wang

“…Because of its high infectivity in humans and the lack of effective vaccines, Nipah virus is classified as a category C agent and handling has to be performed under biosafety level 4 conditions in non-endemic countries, which has hindered the development of vaccines. …”
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Discovery of novel antifungal drugs via screening repurposing libraries against Coccidioides posadasii spherule initials by Sarah Saeger, Kathryn West-Jeppson, Yu-Rou Liao, Althea Campuzano, Jieh-Juen Yu, Jose Lopez-Ribot, Chiung-Yu Hung

“…Additionally, we explored the potential to use an attenuated, Biosafety Level 2 containment mutant, C. posadasii ∆cts2/∆ard1/∆cts3 (∆T), as a surrogate model for drug screening. …”
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Francisella philomiragia Adenitis and Pulmonary Nodules in a Child with Chronic Granulomatous Disease by Timothy Mailman, Matthias H Schmidt

“…Limited awareness of F philomiragia has led to delayed identification, patient death and misidentification as Francisella tularensis - a biosafety level three pathogen and potential bioterrorism agent.…”
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Isolation of vaccinia MVA recombinants using the viral F13L gene as the selective marker by Juana M. Sánchez-Puig, Rafael Blasco

“…Also, as a eukaryotic gene expression vector, MVA can be used in a biosafety level 1 setup, in contrast to more virulent vaccinia virus strains. …”
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TAT-CRE inhalation enables tumor induction corresponding to adenoviral Cre-recombinase in a lung cancer mouse model by Tabea Gewalt, Anna M. Dmitrieva, Felix Elsner, Xinlei Zhao, Daniel Dimitri Sieber, Ilayda Gülsen Kocak, Qian Yang, Claudia Viktoria Orschel, Naja Maria Eckert, Bianca Goebel, Marieke Nill, Franziska Peter, Arndt Hartmann, Filippo Beleggia, Margarete Odenthal, Hans Christian Reinhardt, Roland Tillmann Ullrich, Frederik Graw, Lydia Meder

“…In this context, we perform a comprehensive comparison between TAT-CRE (biosafety level S1) and adenoviral Cre-recombinase induced (biosafety level S2) lung adenocarcinomas driven by Kras G12D expression and Trp53 depletion. …”
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Commentary: Evaluation of post-COVID mortality risk in cases classified as severe acute respiratory syndrome in Brazil: a longitudinal study for medium and long term by Rubens C. Costa-Filho, Rubens C. Costa-Filho, José Paulo G. Leite, Marco Aurélio Horta, Felipe Gomes Naveca, Felipe Gomes Naveca, Felipe Saddy, Hugo C. Castro de Faria Neto, Hugo C. Castro de Faria Neto

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Novel derivatives of brincidofovir and (S)-9-(3-hydroxy-2-phosphonylmethoxypropyl)adenine inhibit orthopoxviruses and human adenoviruses more potently than brincidofovir by Yifan Zhang, Yanmin Wan, Cuiyuan Guo, Zhaoqin Zhu, Chao Qiu, Jiasheng Lu, Yanan Zhou, Jiaojiao Zheng, Fahui Dai, Xiaoyang Cheng, Kunlu Deng, Wanhai Wang, Youchun Wang, Wenhong Zhang

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Validated Methods for Inactivation of Tick-Borne Encephalitis Virus Compatible with Immune-Based and Enzymatic Downstream Analyses by Simone Leoni, Stephen L. Leib, Katharina Summermatter, Denis Grandgirard

“…Since TBEV is classified as a risk group 3 organism in Switzerland, handling infectious material containing the virus is restricted to biosafety level 3 laboratories. In some instances, downstream analyses may need to be performed outside of the containment facility. …”
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