Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats
Dysregulation of calcium signaling is a hallmark of diabetes mellitus (DM) and grain amaranth (AG) has antidiabetic properties. Information on the mechanism of action of AG on blood, renal, and hepatic tissues is sparse, although it continues to be an important alternative medicinal plant in several...
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Kabale University
2023
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| Online Access: | http://hdl.handle.net/20.500.12493/910 |
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| author | Keneth Iceland, Kasozi Namubiru, Sarah Safiriyu, Abass Alao Ninsiima, Herbert Izo Nakimbugwe, Dorothy Namayanja, Monica Valladares, Miriela Betancourt |
| author_facet | Keneth Iceland, Kasozi Namubiru, Sarah Safiriyu, Abass Alao Ninsiima, Herbert Izo Nakimbugwe, Dorothy Namayanja, Monica Valladares, Miriela Betancourt |
| author_sort | Keneth Iceland, Kasozi |
| collection | KAB-DR |
| description | Dysregulation of calcium signaling is a hallmark of diabetes mellitus (DM) and grain amaranth (AG) has antidiabetic properties. Information on the mechanism of action of AG on blood, renal, and hepatic tissues is sparse, although it continues to be an important alternative medicinal plant in several developing countries. The objective of the study was to determine key changes in calcium levels and s100a1 protein levels and antioxidant and histopathologic changes in blood, renal, and hepatic tissues of male diabetic Wistar rats. Materials and Methods. This was an experimental study in which 30 male Wistar rats were kept for
5 weeks (6 groups, N =5). Groups 1-IV had T2DM induced using Nicotinamide and Streptozotocin: Group I, Mixtard ; group
II, positive control; group III, 25% AG; group IV, 50% AG. Furthermore, group V consisted of normal rats given 50% GA and
group VI was negative control. Blood, renal, and hepatic tissues were collected and analyzed for calcium, s100a1 protein levels, and
antioxidant and histopathological changes. Results andDiscussion. Inblood, renal, andhepatic tissue, calciumand s100a1 levelswere
low during T2DMand these increased following AG supplementation.This was important for improved metabolic processes, thus
leading to the lowmalondialdehyde (MDA) and glutathione peroxidase (GPx) activity in the tissues. Efficient antioxidant status was important for improved calciumsignaling mechanisms, thus leading to improved tissue function and protection demonstrating the importance of AG as an alternativemedicinal source through the calciumsignaling pathway. Conclusion. Grain amaranth exerts its antidiabetic properties through improved calcium homeostasis in blood, kidney, and liver. |
| format | Article |
| id | oai:idr.kab.ac.ug:20.500.12493-910 |
| institution | KAB-DR |
| language | en_US |
| publishDate | 2023 |
| publisher | Kabale University |
| record_format | dspace |
| spelling | oai:idr.kab.ac.ug:20.500.12493-9102024-01-17T04:44:30Z Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats Keneth Iceland, Kasozi Namubiru, Sarah Safiriyu, Abass Alao Ninsiima, Herbert Izo Nakimbugwe, Dorothy Namayanja, Monica Valladares, Miriela Betancourt Grain Amaranth Hepatic Renal Calcium Metabolism Diabetes Mellitus Male Wistar Rats Dysregulation of calcium signaling is a hallmark of diabetes mellitus (DM) and grain amaranth (AG) has antidiabetic properties. Information on the mechanism of action of AG on blood, renal, and hepatic tissues is sparse, although it continues to be an important alternative medicinal plant in several developing countries. The objective of the study was to determine key changes in calcium levels and s100a1 protein levels and antioxidant and histopathologic changes in blood, renal, and hepatic tissues of male diabetic Wistar rats. Materials and Methods. This was an experimental study in which 30 male Wistar rats were kept for 5 weeks (6 groups, N =5). Groups 1-IV had T2DM induced using Nicotinamide and Streptozotocin: Group I, Mixtard ; group II, positive control; group III, 25% AG; group IV, 50% AG. Furthermore, group V consisted of normal rats given 50% GA and group VI was negative control. Blood, renal, and hepatic tissues were collected and analyzed for calcium, s100a1 protein levels, and antioxidant and histopathological changes. Results andDiscussion. Inblood, renal, andhepatic tissue, calciumand s100a1 levelswere low during T2DMand these increased following AG supplementation.This was important for improved metabolic processes, thus leading to the lowmalondialdehyde (MDA) and glutathione peroxidase (GPx) activity in the tissues. Efficient antioxidant status was important for improved calciumsignaling mechanisms, thus leading to improved tissue function and protection demonstrating the importance of AG as an alternativemedicinal source through the calciumsignaling pathway. Conclusion. Grain amaranth exerts its antidiabetic properties through improved calcium homeostasis in blood, kidney, and liver. 2023-02-01T03:44:09Z 2023-02-01T03:44:09Z 2018 Article http://hdl.handle.net/20.500.12493/910 en_US application/pdf Kabale University |
| spellingShingle | Grain Amaranth Hepatic Renal Calcium Metabolism Diabetes Mellitus Male Wistar Rats Keneth Iceland, Kasozi Namubiru, Sarah Safiriyu, Abass Alao Ninsiima, Herbert Izo Nakimbugwe, Dorothy Namayanja, Monica Valladares, Miriela Betancourt Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats |
| title | Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats |
| title_full | Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats |
| title_fullStr | Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats |
| title_full_unstemmed | Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats |
| title_short | Grain Amaranth Is Associated with Improved Hepatic and Renal Calcium Metabolism in Type 2 Diabetes Mellitus of Male Wistar Rats |
| title_sort | grain amaranth is associated with improved hepatic and renal calcium metabolism in type 2 diabetes mellitus of male wistar rats |
| topic | Grain Amaranth Hepatic Renal Calcium Metabolism Diabetes Mellitus Male Wistar Rats |
| url | http://hdl.handle.net/20.500.12493/910 |
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