In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants

Background Codonopsis pilosula (Franch.) Nannf. is a medicinal plant traditionally used in China, Korea, and Japan to treat many diseases including poor gastrointestinal function, low immunity, gastric ulcers, and chronic gastritis. The increasing therapeutic and preventive use of C. pilosula has...

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Main Authors: Roggers, Gang, Richard, Komakech, Yuseong, Chung, Denis, Okello, Wook, Jin Kim, Byeong, Cheol Moon, Nam, Hui Yim, Youngmin, Kang
Format: Article
Language:English
Published: BMC Plant Biology 2023
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Online Access:http://hdl.handle.net/20.500.12493/899
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author Roggers, Gang
Richard, Komakech
Yuseong, Chung
Denis, Okello
Wook, Jin Kim
Byeong, Cheol Moon
Nam, Hui Yim
Youngmin, Kang
author_facet Roggers, Gang
Richard, Komakech
Yuseong, Chung
Denis, Okello
Wook, Jin Kim
Byeong, Cheol Moon
Nam, Hui Yim
Youngmin, Kang
author_sort Roggers, Gang
collection KAB-DR
description Background Codonopsis pilosula (Franch.) Nannf. is a medicinal plant traditionally used in China, Korea, and Japan to treat many diseases including poor gastrointestinal function, low immunity, gastric ulcers, and chronic gastritis. The increasing therapeutic and preventive use of C. pilosula has subsequently led to depletion of the natural populations of this species thus necessitating propagation of this important medicinal plant. Here, we developed an efficient and effective in vitro propagation protocol for C. pilosula using apical shoot segments. We tested various plant tissue culture media for the growth of C. pilosula and evaluated the effects of plant growth regulators on the shoot pro‑ liferation and rooting of regenerated C. pilosula plants. Furthermore, the tissues (roots and shoots) of maternal and in vitro‑regenerated C. pilosula plants were subjected to Fourier‑transform near‑infrared (FT‑NIR) spectrometry, Gas chromatography‑mass spectrometry (GC–MS), and their total flavonoids, phenolics, and antioxidant capacity were determined and compared. Results Full‑strength Murashige and Skoog (MS) medium augmented with vitamins and benzylaminopurine (1.5 mg·L−1 ) regenerated the highest shoot number (12 ± 0.46) per explant. MS medium augmented with indole‑ 3‑acetic acid (1.0 mg·L−1 ) produced the highest root number (9 ± 0.89) and maximum root length (20.88 ± 1.48 mm) from regenerated C. pilosula shoots. The survival rate of in vitro-regenerated C. pilosula plants was 94.00% after acclimatization. The maternal and in vitro‑regenerated C. pilosula plant tissues showed similar FT‑NIR spectra, total phenolics, total flavonoids, phytochemical composition, and antioxidant activity. Randomly amplified polymorphic DNA (RAPD) test confirmed the genetic fidelity of regenerated C. pilosula plants. Conclusions The proposed in vitro propagation protocol may be useful for the rapid mass multiplication and production of high quality C. pilosula as well as for germplasm preservation to ensure sustainable supply amidst the ever‑increasing demand
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spelling oai:idr.kab.ac.ug:20.500.12493-8992024-01-17T04:44:39Z In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants Roggers, Gang Richard, Komakech Yuseong, Chung Denis, Okello Wook, Jin Kim Byeong, Cheol Moon Nam, Hui Yim Youngmin, Kang Plant tissue culture, Plant growth regulators, FT‑NIR, Genetic fidelity, Phenolics, Flavonoids, GC–MS and Antioxidant activity Background Codonopsis pilosula (Franch.) Nannf. is a medicinal plant traditionally used in China, Korea, and Japan to treat many diseases including poor gastrointestinal function, low immunity, gastric ulcers, and chronic gastritis. The increasing therapeutic and preventive use of C. pilosula has subsequently led to depletion of the natural populations of this species thus necessitating propagation of this important medicinal plant. Here, we developed an efficient and effective in vitro propagation protocol for C. pilosula using apical shoot segments. We tested various plant tissue culture media for the growth of C. pilosula and evaluated the effects of plant growth regulators on the shoot pro‑ liferation and rooting of regenerated C. pilosula plants. Furthermore, the tissues (roots and shoots) of maternal and in vitro‑regenerated C. pilosula plants were subjected to Fourier‑transform near‑infrared (FT‑NIR) spectrometry, Gas chromatography‑mass spectrometry (GC–MS), and their total flavonoids, phenolics, and antioxidant capacity were determined and compared. Results Full‑strength Murashige and Skoog (MS) medium augmented with vitamins and benzylaminopurine (1.5 mg·L−1 ) regenerated the highest shoot number (12 ± 0.46) per explant. MS medium augmented with indole‑ 3‑acetic acid (1.0 mg·L−1 ) produced the highest root number (9 ± 0.89) and maximum root length (20.88 ± 1.48 mm) from regenerated C. pilosula shoots. The survival rate of in vitro-regenerated C. pilosula plants was 94.00% after acclimatization. The maternal and in vitro‑regenerated C. pilosula plant tissues showed similar FT‑NIR spectra, total phenolics, total flavonoids, phytochemical composition, and antioxidant activity. Randomly amplified polymorphic DNA (RAPD) test confirmed the genetic fidelity of regenerated C. pilosula plants. Conclusions The proposed in vitro propagation protocol may be useful for the rapid mass multiplication and production of high quality C. pilosula as well as for germplasm preservation to ensure sustainable supply amidst the ever‑increasing demand Kabale University 2023-01-20T12:16:59Z 2023-01-20T12:16:59Z 2023 Article https://doi.org/10.1186/s12870-022-03950-w http://hdl.handle.net/20.500.12493/899 en Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf BMC Plant Biology
spellingShingle Plant tissue culture,
Plant growth regulators,
FT‑NIR, Genetic fidelity,
Phenolics, Flavonoids,
GC–MS
and Antioxidant activity
Roggers, Gang
Richard, Komakech
Yuseong, Chung
Denis, Okello
Wook, Jin Kim
Byeong, Cheol Moon
Nam, Hui Yim
Youngmin, Kang
In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
title In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
title_full In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
title_fullStr In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
title_full_unstemmed In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
title_short In vitro propagation of Codonopsis pilosula (Franch.) Nannf. using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
title_sort in vitro propagation of codonopsis pilosula franch nannf using apical shoot segments and phytochemical assessments of the maternal and regenerated plants
topic Plant tissue culture,
Plant growth regulators,
FT‑NIR, Genetic fidelity,
Phenolics, Flavonoids,
GC–MS
and Antioxidant activity
url http://hdl.handle.net/20.500.12493/899
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