Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1
Background: Bisphenol A (BPA) is a rapid spreading organic pollutant that widely used in many industries espe‑ cially as a plasticizer in polycarbonate plastic and epoxy resins. BPA reported as a prominent endocrine disruptor compound that possesses estrogenic activity and fulminant toxicity. Pseu...
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BMC Microbiology
2023
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| Online Access: | http://hdl.handle.net/20.500.12493/898 |
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| author | Adel, Eltoukhy Yang, Jia Imane, Lamraoui M. A., Abo‑Kadoum Omar, Mohammad Atta Ruth, Nahurira Junhuan, Wang Yanchun, Yan |
| author_facet | Adel, Eltoukhy Yang, Jia Imane, Lamraoui M. A., Abo‑Kadoum Omar, Mohammad Atta Ruth, Nahurira Junhuan, Wang Yanchun, Yan |
| author_sort | Adel, Eltoukhy |
| collection | KAB-DR |
| description | Background: Bisphenol A (BPA) is a rapid spreading organic pollutant that widely used in many industries espe‑
cially as a plasticizer in polycarbonate plastic and epoxy resins. BPA reported as a prominent endocrine disruptor
compound that possesses estrogenic activity and fulminant toxicity. Pseudomonas putida YC‑AE1 was isolated in our
previous study and exerted a strong degradation capacity toward BPA at high concentrations; however, the molecular
degradation mechanism is still enigmatic.
Results: We employed RNA sequencing to analyze the differentially expressed genes (DEGs) in the YC‑AE1 strain
upon BPA induction. Out of 1229 differentially expressed genes, 725 genes were positively regulated, and 504 genes
were down‑regulated. The pathways of microbial metabolism in diverse environments were significantly enriched
among DEGs based on KEGG enrichment analysis. qRT‑PCR confirm the involvement of BPA degradation relevant
genes in accordance with RNA Seq data. The degradation pathway of BPA in YC‑AE1 was proposed with specific
enzymes and encoded genes. The role of cytochrome P450 (CYP450) in BPA degradation was further verified. Sever
decrease in BPA degradation was recorded by YC‑AE1 in the presence of CYP450 inhibitor. Subsequently, CYP‑
450bisdB deficient YC‑AE1 strain △ bisdB lost its ability toward BPA transformation comparing with the wild type.
Furthermore, Transformation of E. coli with pET‑32a‑bisdAB empowers it to degrade 66 mg l−1 of BPA after 24 h. Alto‑
gether, the results showed the role of CYP450 in biodegradation of BPA by YC‑AE1.
Conclusion: In this study we propose the molecular basis and the potential role of YC‑AE1cytochrome P450
monooxygenase in BPA catabolism |
| format | Article |
| id | oai:idr.kab.ac.ug:20.500.12493-898 |
| institution | KAB-DR |
| language | English |
| publishDate | 2023 |
| publisher | BMC Microbiology |
| record_format | dspace |
| spelling | oai:idr.kab.ac.ug:20.500.12493-8982024-01-17T04:44:07Z Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 Adel, Eltoukhy Yang, Jia Imane, Lamraoui M. A., Abo‑Kadoum Omar, Mohammad Atta Ruth, Nahurira Junhuan, Wang Yanchun, Yan Bisphenol A Pseudomonas putida YC‑AE1 Cytochrome P450 Degradation pathway RNA sequencing Background: Bisphenol A (BPA) is a rapid spreading organic pollutant that widely used in many industries espe‑ cially as a plasticizer in polycarbonate plastic and epoxy resins. BPA reported as a prominent endocrine disruptor compound that possesses estrogenic activity and fulminant toxicity. Pseudomonas putida YC‑AE1 was isolated in our previous study and exerted a strong degradation capacity toward BPA at high concentrations; however, the molecular degradation mechanism is still enigmatic. Results: We employed RNA sequencing to analyze the differentially expressed genes (DEGs) in the YC‑AE1 strain upon BPA induction. Out of 1229 differentially expressed genes, 725 genes were positively regulated, and 504 genes were down‑regulated. The pathways of microbial metabolism in diverse environments were significantly enriched among DEGs based on KEGG enrichment analysis. qRT‑PCR confirm the involvement of BPA degradation relevant genes in accordance with RNA Seq data. The degradation pathway of BPA in YC‑AE1 was proposed with specific enzymes and encoded genes. The role of cytochrome P450 (CYP450) in BPA degradation was further verified. Sever decrease in BPA degradation was recorded by YC‑AE1 in the presence of CYP450 inhibitor. Subsequently, CYP‑ 450bisdB deficient YC‑AE1 strain △ bisdB lost its ability toward BPA transformation comparing with the wild type. Furthermore, Transformation of E. coli with pET‑32a‑bisdAB empowers it to degrade 66 mg l−1 of BPA after 24 h. Alto‑ gether, the results showed the role of CYP450 in biodegradation of BPA by YC‑AE1. Conclusion: In this study we propose the molecular basis and the potential role of YC‑AE1cytochrome P450 monooxygenase in BPA catabolism Kabale University 2023-01-17T14:01:03Z 2023-01-17T14:01:03Z 2022 Article https://doi.org/10.1186/s12866-022-02689-6 http://hdl.handle.net/20.500.12493/898 en Attribution-NonCommercial-NoDerivs 3.0 United States http://creativecommons.org/licenses/by-nc-nd/3.0/us/ application/pdf BMC Microbiology |
| spellingShingle | Bisphenol A Pseudomonas putida YC‑AE1 Cytochrome P450 Degradation pathway RNA sequencing Adel, Eltoukhy Yang, Jia Imane, Lamraoui M. A., Abo‑Kadoum Omar, Mohammad Atta Ruth, Nahurira Junhuan, Wang Yanchun, Yan Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 |
| title | Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 |
| title_full | Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 |
| title_fullStr | Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 |
| title_full_unstemmed | Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 |
| title_short | Transcriptome analysis and cytochrome P450 monooxygenase reveal the molecular mechanism of Bisphenol A degradation by Pseudomonas putida strain YC-AE1 |
| title_sort | transcriptome analysis and cytochrome p450 monooxygenase reveal the molecular mechanism of bisphenol a degradation by pseudomonas putida strain yc ae1 |
| topic | Bisphenol A Pseudomonas putida YC‑AE1 Cytochrome P450 Degradation pathway RNA sequencing |
| url | http://hdl.handle.net/20.500.12493/898 |
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