Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda
Uganda is among the 22 countries in the world with a high burden of tuberculosis. The southwestern region of the country has consistently registered a high TB/HIV incidence rate. This study is aimed at characterizing the Mycobacterium tuberculosis complex (MTBC) genotypic diversity in southwestern...
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Hindawi
2021
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| Online Access: | http://hdl.handle.net/20.500.12493/512 |
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| author | Lisa, Nkatha Micheni Kennedy, Kassaza Hellen, W. Kinyi |
| author_facet | Lisa, Nkatha Micheni Kennedy, Kassaza Hellen, W. Kinyi |
| author_sort | Lisa, Nkatha Micheni |
| collection | KAB-DR |
| description | Uganda is among the 22 countries in the world with a high burden of tuberculosis. The southwestern region of the country has
consistently registered a high TB/HIV incidence rate. This study is aimed at characterizing the Mycobacterium tuberculosis
complex (MTBC) genotypic diversity in southwestern Uganda. A total of 283 sputum samples from patients with pulmonary
tuberculosis were genotyped using specific single nucleotide polymorphism markers for lineages 3 and 4. Most of the patients
were males with a mean age of 34. The lineage 4 Ugandan family was found to be the most dominant strains accounting for
59.7% of all cases followed by lineage 3 at 15.2%. The lineage 4 non-Ugandan family accounted for 14.5% of all cases while 4.2% showed amplification for both lineage 4 and lineage 3. Eighteen samples (6.4%) of the strains remained unclassified since they could not be matched to any lineage based on the genotyping technique used. This study demonstrates that a wide diversity of strains is causing pulmonary tuberculosis in this region with those belonging to the lineage 4 Ugandan family being more predominant. However, to confirm this, further studies using more discriminative genotyping methods are necessary. |
| format | Article |
| id | oai:idr.kab.ac.ug:20.500.12493-512 |
| institution | KAB-DR |
| publishDate | 2021 |
| publisher | Hindawi |
| record_format | dspace |
| spelling | oai:idr.kab.ac.ug:20.500.12493-5122024-01-17T04:46:10Z Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda Lisa, Nkatha Micheni Kennedy, Kassaza Hellen, W. Kinyi Mycobacterium tuberculosis, Pulmonary Tuberculosis Uganda is among the 22 countries in the world with a high burden of tuberculosis. The southwestern region of the country has consistently registered a high TB/HIV incidence rate. This study is aimed at characterizing the Mycobacterium tuberculosis complex (MTBC) genotypic diversity in southwestern Uganda. A total of 283 sputum samples from patients with pulmonary tuberculosis were genotyped using specific single nucleotide polymorphism markers for lineages 3 and 4. Most of the patients were males with a mean age of 34. The lineage 4 Ugandan family was found to be the most dominant strains accounting for 59.7% of all cases followed by lineage 3 at 15.2%. The lineage 4 non-Ugandan family accounted for 14.5% of all cases while 4.2% showed amplification for both lineage 4 and lineage 3. Eighteen samples (6.4%) of the strains remained unclassified since they could not be matched to any lineage based on the genotyping technique used. This study demonstrates that a wide diversity of strains is causing pulmonary tuberculosis in this region with those belonging to the lineage 4 Ugandan family being more predominant. However, to confirm this, further studies using more discriminative genotyping methods are necessary. Kabale University 2021-07-30T16:58:55Z 2021-07-30T16:58:55Z 2021 Article https://doi.org/10.1155/2021/5588339 http://hdl.handle.net/20.500.12493/512 ;5588339 application/pdf Hindawi |
| spellingShingle | Mycobacterium tuberculosis, Pulmonary Tuberculosis Lisa, Nkatha Micheni Kennedy, Kassaza Hellen, W. Kinyi Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda |
| title | Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda |
| title_full | Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda |
| title_fullStr | Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda |
| title_full_unstemmed | Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda |
| title_short | Diversity of Mycobacterium tuberculosis Complex Lineages Associated with Pulmonary Tuberculosis in Southwestern, Uganda |
| title_sort | diversity of mycobacterium tuberculosis complex lineages associated with pulmonary tuberculosis in southwestern uganda |
| topic | Mycobacterium tuberculosis, Pulmonary Tuberculosis |
| url | http://hdl.handle.net/20.500.12493/512 |
| work_keys_str_mv | AT lisankathamicheni diversityofmycobacteriumtuberculosiscomplexlineagesassociatedwithpulmonarytuberculosisinsouthwesternuganda AT kennedykassaza diversityofmycobacteriumtuberculosiscomplexlineagesassociatedwithpulmonarytuberculosisinsouthwesternuganda AT hellenwkinyi diversityofmycobacteriumtuberculosiscomplexlineagesassociatedwithpulmonarytuberculosisinsouthwesternuganda |