Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica.
The leaves of Alstonia boonei and aerial parts of Ipomoea cairica are used for treatment of microbial infections among other ailments in African traditional medicine. The aim of this study was to investigate the antimicrobial phytochemicals in A. boonei leaves and Ipomoea cairica aerial parts to val...
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Kabale University
2024
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| Online Access: | http://hdl.handle.net/20.500.12493/2325 |
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| author | Gumula, Ivan Kyarimpa, Christine Nanyonga, Sarah Kiwanuka Kwesiga, George Busulwa, George Opio, Boniface Heydenreich, Mathias Omara, Timothy |
| author_facet | Gumula, Ivan Kyarimpa, Christine Nanyonga, Sarah Kiwanuka Kwesiga, George Busulwa, George Opio, Boniface Heydenreich, Mathias Omara, Timothy |
| author_sort | Gumula, Ivan |
| collection | KAB-DR |
| description | The leaves of Alstonia boonei and aerial parts of Ipomoea cairica are used for treatment of microbial infections among other ailments in African traditional medicine. The aim of this study was to investigate the antimicrobial phytochemicals in A. boonei leaves and Ipomoea cairica aerial parts to validate their traditional use in Ugandan herbal medicine. Methods: The plant materials were separately extracted using a dichloromethane/methanol (1:1) solvent system and subjected to repeated chromatographic separation to isolate pure compounds. The chemical structures of the isolated compounds were determined through 1 H NMR, 13C NMR and 2D NMR (COSY, HSQC and HMBC). The antibacterial activity of the extracts and pure compounds were assessed using the agar well diffusion method. Results: Chromatographic fractionation of the extracts yielded trans-fagaramide and a pentacyclic lupane-type triterpenoid, lupeol, from A. boonei, and friedelin from I. cairica. Trans-fagaramide was identified for the first time in the Alstonia genus while friedelin was identified for the first time in I. cairica. The isolated compounds demonstrated antibacterial activity, with trans-fagaramide showing a minimum inhibitory concentration (MIC) of 125 μg/mL against Pseudomonas aeruginosa and 250 μg/mL against Staphylococcus aureus, Salmonella typhi and Escherichia coli. Friedelin exhibited a MIC of 125 μg/mL against
Escherichia coli and 250 μg/mL against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. Conclusion: The antibacterial activities observed in this study support the traditional use of A. boonei and I. cairica by indigenous communities in Uganda for treating microbial infections. |
| format | Article |
| id | oai:idr.kab.ac.ug:20.500.12493-2325 |
| institution | KAB-DR |
| language | English |
| publishDate | 2024 |
| publisher | Kabale University |
| record_format | dspace |
| spelling | oai:idr.kab.ac.ug:20.500.12493-23252024-10-19T00:01:16Z Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. Gumula, Ivan Kyarimpa, Christine Nanyonga, Sarah Kiwanuka Kwesiga, George Busulwa, George Opio, Boniface Heydenreich, Mathias Omara, Timothy Alstonia Boonei Antibacterial Friedelin Ipomoea Cairica lupeol Trans-Fagaramide The leaves of Alstonia boonei and aerial parts of Ipomoea cairica are used for treatment of microbial infections among other ailments in African traditional medicine. The aim of this study was to investigate the antimicrobial phytochemicals in A. boonei leaves and Ipomoea cairica aerial parts to validate their traditional use in Ugandan herbal medicine. Methods: The plant materials were separately extracted using a dichloromethane/methanol (1:1) solvent system and subjected to repeated chromatographic separation to isolate pure compounds. The chemical structures of the isolated compounds were determined through 1 H NMR, 13C NMR and 2D NMR (COSY, HSQC and HMBC). The antibacterial activity of the extracts and pure compounds were assessed using the agar well diffusion method. Results: Chromatographic fractionation of the extracts yielded trans-fagaramide and a pentacyclic lupane-type triterpenoid, lupeol, from A. boonei, and friedelin from I. cairica. Trans-fagaramide was identified for the first time in the Alstonia genus while friedelin was identified for the first time in I. cairica. The isolated compounds demonstrated antibacterial activity, with trans-fagaramide showing a minimum inhibitory concentration (MIC) of 125 μg/mL against Pseudomonas aeruginosa and 250 μg/mL against Staphylococcus aureus, Salmonella typhi and Escherichia coli. Friedelin exhibited a MIC of 125 μg/mL against Escherichia coli and 250 μg/mL against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. Conclusion: The antibacterial activities observed in this study support the traditional use of A. boonei and I. cairica by indigenous communities in Uganda for treating microbial infections. 2024-10-18T07:44:45Z 2024-10-18T07:44:45Z 2024 Article Gumula, Ivan et al. (2024). Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. Kabale: Kabale University. http://hdl.handle.net/20.500.12493/2325 en application/pdf Kabale University |
| spellingShingle | Alstonia Boonei Antibacterial Friedelin Ipomoea Cairica lupeol Trans-Fagaramide Gumula, Ivan Kyarimpa, Christine Nanyonga, Sarah Kiwanuka Kwesiga, George Busulwa, George Opio, Boniface Heydenreich, Mathias Omara, Timothy Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. |
| title | Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. |
| title_full | Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. |
| title_fullStr | Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. |
| title_full_unstemmed | Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. |
| title_short | Antibacterial Properties of Phytochemicals Isolated from Leaves of Alstonia boonei and Aerial Parts of Ipomoea cairica. |
| title_sort | antibacterial properties of phytochemicals isolated from leaves of alstonia boonei and aerial parts of ipomoea cairica |
| topic | Alstonia Boonei Antibacterial Friedelin Ipomoea Cairica lupeol Trans-Fagaramide |
| url | http://hdl.handle.net/20.500.12493/2325 |
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