In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities.
Apios americana Medik tissues, including tubers, flowers, leaves, and vines have both nutritional and therapeutic benefits. These tissues are extensively used in products like beverages and food supplements, driving an exponential increase in worldwide demand for the plant. However, conventional pro...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | en_US |
| Published: |
Kabale University
2024
|
| Subjects: | |
| Online Access: | http://hdl.handle.net/20.500.12493/1970 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1813635239702953984 |
|---|---|
| author | Okello, Denis Gang, Roggers Rahmat, Endang Yang, Sungyu Ban, Yeongjun Chung, Yuseong Lee, Jun Kang, Youngmin |
| author_facet | Okello, Denis Gang, Roggers Rahmat, Endang Yang, Sungyu Ban, Yeongjun Chung, Yuseong Lee, Jun Kang, Youngmin |
| author_sort | Okello, Denis |
| collection | KAB-DR |
| description | Apios americana Medik tissues, including tubers, flowers, leaves, and vines have both nutritional and therapeutic benefits. These tissues are extensively used in products like beverages and food supplements, driving an exponential increase in worldwide demand for the plant. However, conventional propagation of A. Americana through seeds and tubers faces several challenges, including limited seed sets under manual pollination, resulting in reduced production. To overcome these challenges, micropropagation emerges as a promising solution. The current research study has developed an effective in vitro propagation strategy for A. americana using direct regeneration from apical segment explants. Full-strength Quoirin and Lepoivre (QL) medium, supplemented with vitamins and 2.0 mg/L benzylaminopurine (BAP), yielded the highest number of shoots (7.50 ± 0.06) per apical segment explant. QL medium fortified with 1.0 mg/L indole-3-butyric acid (IBA) demonstrated the highest root number (2.80 ± 0.30), length (85.80 ± 7.49 mm), and surface area (10.40 ± 0.82 cm2) in regenerated A. American shoots. The survival rate of the regenerants in the ex-vitro environment was 92.7% after acclimatization. In vitro-regenerated A. Americana plants and mother plants exhibited similar ploidy levels, chlorophyll content, photosynthetic rate, phytochemical composition, and antioxidant capacity, indicating similarity in their physiological and biochemical properties. This protocol offers a rapid and scalable method for generating high-quality, A. Americana plant stocks with stable genome size/ploidy level, meeting the exponentially growing demand for plant tissues. Additionally, it holds potential applications in A. Americana breeding and germplasm
preservation. |
| format | Article |
| id | oai:idr.kab.ac.ug:20.500.12493-1970 |
| institution | KAB-DR |
| language | en_US |
| publishDate | 2024 |
| publisher | Kabale University |
| record_format | dspace |
| spelling | oai:idr.kab.ac.ug:20.500.12493-19702024-08-01T00:01:57Z In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. Okello, Denis Gang, Roggers Rahmat, Endang Yang, Sungyu Ban, Yeongjun Chung, Yuseong Lee, Jun Kang, Youngmin Apios americana in vitro propagation Apical segment Phytochemical composition Antioxidant activity Flow cytometry Apios americana Medik tissues, including tubers, flowers, leaves, and vines have both nutritional and therapeutic benefits. These tissues are extensively used in products like beverages and food supplements, driving an exponential increase in worldwide demand for the plant. However, conventional propagation of A. Americana through seeds and tubers faces several challenges, including limited seed sets under manual pollination, resulting in reduced production. To overcome these challenges, micropropagation emerges as a promising solution. The current research study has developed an effective in vitro propagation strategy for A. americana using direct regeneration from apical segment explants. Full-strength Quoirin and Lepoivre (QL) medium, supplemented with vitamins and 2.0 mg/L benzylaminopurine (BAP), yielded the highest number of shoots (7.50 ± 0.06) per apical segment explant. QL medium fortified with 1.0 mg/L indole-3-butyric acid (IBA) demonstrated the highest root number (2.80 ± 0.30), length (85.80 ± 7.49 mm), and surface area (10.40 ± 0.82 cm2) in regenerated A. American shoots. The survival rate of the regenerants in the ex-vitro environment was 92.7% after acclimatization. In vitro-regenerated A. Americana plants and mother plants exhibited similar ploidy levels, chlorophyll content, photosynthetic rate, phytochemical composition, and antioxidant capacity, indicating similarity in their physiological and biochemical properties. This protocol offers a rapid and scalable method for generating high-quality, A. Americana plant stocks with stable genome size/ploidy level, meeting the exponentially growing demand for plant tissues. Additionally, it holds potential applications in A. Americana breeding and germplasm preservation. 2024-04-09T08:43:06Z 2024-04-09T08:43:06Z 2024 Article Okello, Denis et al. (2024). In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities.Kabale: Kabale University. http://hdl.handle.net/20.500.12493/1970 en_US application/pdf Kabale University |
| spellingShingle | Apios americana in vitro propagation Apical segment Phytochemical composition Antioxidant activity Flow cytometry Okello, Denis Gang, Roggers Rahmat, Endang Yang, Sungyu Ban, Yeongjun Chung, Yuseong Lee, Jun Kang, Youngmin In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. |
| title | In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. |
| title_full | In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. |
| title_fullStr | In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. |
| title_full_unstemmed | In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. |
| title_short | In vitro multiplication and phytochemical evaluation of Apios americana Medik for enhanced production of the staple food and tissues with versatile bioactivities. |
| title_sort | in vitro multiplication and phytochemical evaluation of apios americana medik for enhanced production of the staple food and tissues with versatile bioactivities |
| topic | Apios americana in vitro propagation Apical segment Phytochemical composition Antioxidant activity Flow cytometry |
| url | http://hdl.handle.net/20.500.12493/1970 |
| work_keys_str_mv | AT okellodenis invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT gangroggers invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT rahmatendang invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT yangsungyu invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT banyeongjun invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT chungyuseong invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT leejun invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities AT kangyoungmin invitromultiplicationandphytochemicalevaluationofapiosamericanamedikforenhancedproductionofthestaplefoodandtissueswithversatilebioactivities |