In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda
The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS patients’...
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2019
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| Online Access: | http://hdl.handle.net/20.500.12493/171 |
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| author | Joseph Obiezu, Chukwujekwu Ezeonwumelu Muhammad, Ntale Steve, Okwudili Ogbonnia Ezera, Agwu Julius, Kihdze Tanayen Keneth Iceland, Kasozi Okonkwo, Chukwudi Onyeka Anthonia, Shodunke Ambrose, Amamchukwu Akunne Onokiojare, Ephraim Dafiewhare Jennifer, Chibuogwu Ebosie Frederick, Byarugaba |
| author_facet | Joseph Obiezu, Chukwujekwu Ezeonwumelu Muhammad, Ntale Steve, Okwudili Ogbonnia Ezera, Agwu Julius, Kihdze Tanayen Keneth Iceland, Kasozi Okonkwo, Chukwudi Onyeka Anthonia, Shodunke Ambrose, Amamchukwu Akunne Onokiojare, Ephraim Dafiewhare Jennifer, Chibuogwu Ebosie Frederick, Byarugaba |
| author_sort | Joseph Obiezu, Chukwujekwu Ezeonwumelu |
| collection | KAB-DR |
| description | The objective of the study was to determine the antibacterial efficacy of Bidens
pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides
Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave
Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS
patients’ oral bacteria. Healthy green leaves of the plants were collected in
Ishaka Uganda, processed and portions separately extracted with hot distilled
water and cold ethanol. The susceptibility, MIC and MBC of each extract were
determined using standard protocols. The bacteria had significant (p < 0.05)
respective total susceptibilities of 35 [28.7%] to BPA; 42 [34.4%] to BPE; 61
[50.0%] to ACA; 45 [36.9%] to ACE; 38 [31.1%] to OSA; 32 [26.3%] to OSE;
105 (86.0%)] to ceftriaxone. BPE, ACA, OSA, OSE and ceftriaxone had significant
MIC with [F(1, 13); P = 0.00 and BPA with F(1, 13); P = 0.03]. BPE,
ACA, ACE, OSA and ceftriaxone had significant MBC with [F(1, 13); P = 0.00
and BPA with F(1, 13); P = 0.01] on the test bacteria (MANOVA). These
tested medicinal plants’ extracts and ceftriaxone had significant activity
against oral bacteria with ACA having the best activity when compared with
the control. However, the plants’ extracts were resisted by some of the bacteria.
These findings validate the claims of efficacy of Bidens pilosa , Ageratum
conyzoides and Ocimum suave on oral lesions of HIV/AIDS patients made by
traditional healers and local people in South-Western Uganda. We recommend
a detailed study of structural identities and activities of the active antibacterial
principle(s) in these plants for possible new drug entities and verification
of the interactive effects of the principle(s) with ARVs and cotrimoxazole
used daily by HIV/AIDS patients. |
| format | Article |
| id | oai:idr.kab.ac.ug:20.500.12493-171 |
| institution | KAB-DR |
| publishDate | 2019 |
| publisher | Scientific Research Publishing Inc. |
| record_format | dspace |
| spelling | oai:idr.kab.ac.ug:20.500.12493-1712024-01-17T04:48:54Z In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda Joseph Obiezu, Chukwujekwu Ezeonwumelu Muhammad, Ntale Steve, Okwudili Ogbonnia Ezera, Agwu Julius, Kihdze Tanayen Keneth Iceland, Kasozi Okonkwo, Chukwudi Onyeka Anthonia, Shodunke Ambrose, Amamchukwu Akunne Onokiojare, Ephraim Dafiewhare Jennifer, Chibuogwu Ebosie Frederick, Byarugaba Antibacterial Efficacy, Bidens Pilosa, Ageratum Conyzoides, Ocimum Suave, Oral Bacteria, HIV/AIDS, Uganda The objective of the study was to determine the antibacterial efficacy of Bidens pilosa Aqueous (BPA), Bidens pilosa Ethanolic (BPE), Ageratum conyzoides Aqueous (ACA), Ageratum conyzoides Ethanolic (ACE), Ocimum suave Aqueous (OSA) and Ocimum suave Ethanolic (OSE) extracts on HIV/AIDS patients’ oral bacteria. Healthy green leaves of the plants were collected in Ishaka Uganda, processed and portions separately extracted with hot distilled water and cold ethanol. The susceptibility, MIC and MBC of each extract were determined using standard protocols. The bacteria had significant (p < 0.05) respective total susceptibilities of 35 [28.7%] to BPA; 42 [34.4%] to BPE; 61 [50.0%] to ACA; 45 [36.9%] to ACE; 38 [31.1%] to OSA; 32 [26.3%] to OSE; 105 (86.0%)] to ceftriaxone. BPE, ACA, OSA, OSE and ceftriaxone had significant MIC with [F(1, 13); P = 0.00 and BPA with F(1, 13); P = 0.03]. BPE, ACA, ACE, OSA and ceftriaxone had significant MBC with [F(1, 13); P = 0.00 and BPA with F(1, 13); P = 0.01] on the test bacteria (MANOVA). These tested medicinal plants’ extracts and ceftriaxone had significant activity against oral bacteria with ACA having the best activity when compared with the control. However, the plants’ extracts were resisted by some of the bacteria. These findings validate the claims of efficacy of Bidens pilosa , Ageratum conyzoides and Ocimum suave on oral lesions of HIV/AIDS patients made by traditional healers and local people in South-Western Uganda. We recommend a detailed study of structural identities and activities of the active antibacterial principle(s) in these plants for possible new drug entities and verification of the interactive effects of the principle(s) with ARVs and cotrimoxazole used daily by HIV/AIDS patients. Kabale University 2019-06-14T10:02:42Z 2019-06-14T10:02:42Z 2017-09-29 Article Ezeonwumelu, J.O.C., Ntale, M., Ogbonnia, S.O., Agwu, E., Tanayen, J.K., Kasozi, K.I., Okonkwo, C.O., Shodunke, A., Akunne, A.A., Dafiewhare, O.E., Ebosie, J.C. and Byarugaba, F. (2017) In vitro Antibacterial Efficacy of Bidens pilosa , Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda. Pharmacology & Pharmacy , 8, 306-323. http://hdl.handle.net/20.500.12493/171 ;2017, 8, 306-323 application/pdf Scientific Research Publishing Inc. |
| spellingShingle | Antibacterial Efficacy, Bidens Pilosa, Ageratum Conyzoides, Ocimum Suave, Oral Bacteria, HIV/AIDS, Uganda Joseph Obiezu, Chukwujekwu Ezeonwumelu Muhammad, Ntale Steve, Okwudili Ogbonnia Ezera, Agwu Julius, Kihdze Tanayen Keneth Iceland, Kasozi Okonkwo, Chukwudi Onyeka Anthonia, Shodunke Ambrose, Amamchukwu Akunne Onokiojare, Ephraim Dafiewhare Jennifer, Chibuogwu Ebosie Frederick, Byarugaba In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda |
| title | In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda |
| title_full | In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda |
| title_fullStr | In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda |
| title_full_unstemmed | In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda |
| title_short | In vitro Antibacterial Efficacy of Bidens pilosa, Ageratum conyzoides and Ocimum suave Extracts against HIV/AIDS Patients’ Oral Bacteria in South-Western Uganda |
| title_sort | in vitro antibacterial efficacy of bidens pilosa ageratum conyzoides and ocimum suave extracts against hiv aids patients oral bacteria in south western uganda |
| topic | Antibacterial Efficacy, Bidens Pilosa, Ageratum Conyzoides, Ocimum Suave, Oral Bacteria, HIV/AIDS, Uganda |
| url | http://hdl.handle.net/20.500.12493/171 |
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