Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps
Abstract Postmenopause marks a critical physiological transition in women, triggering changes in fat distribution and promoting obesity, which is strongly linked to systemic low-grade chronic inflammation, with neutrophil extracellular traps (NETs) playing a pivotal role in this process. Differentia...
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Nature Portfolio
2025-07-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-06379-x |
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| author | Anzhu Wang Wei Zhang Mengjiao Kang Shan Wang Jingjing Shi Pengfei Xie Linhua Zhao Xiaolin Tong |
| author_facet | Anzhu Wang Wei Zhang Mengjiao Kang Shan Wang Jingjing Shi Pengfei Xie Linhua Zhao Xiaolin Tong |
| author_sort | Anzhu Wang |
| collection | DOAJ |
| description | Abstract Postmenopause marks a critical physiological transition in women, triggering changes in fat distribution and promoting obesity, which is strongly linked to systemic low-grade chronic inflammation, with neutrophil extracellular traps (NETs) playing a pivotal role in this process. Differentially expressed genes (DEGs) related to obesity were identified from the GEO database (GSE44000), followed by protein-protein interaction (PPI) network construction and functional enrichment analysis. Immune infiltration analysis was performed to assess changes in the adipose tissue microenvironment. In vivo, a bilateral ovariectomized (OVX) mouse model was employed to evaluate the effects of adenosine on obesity. RT-qPCR and Western blot were used to assess gene expression and NETs markers. A total of 510 DEGs were identified, with 469 upregulated genes primarily linked to inflammation and immune infiltration. PPI analysis highlighted key genes involved in NETs formation. Immune infiltration analysis revealed significant immune microenvironment alterations. Adenosine treatment in OVX mice reduced body weight, fat tissue, liver lipid deposition, and NETs markers (ELANE, MPO), downregulating TNF, ITGB2, and ITGAM. Adenosine ameliorates postmenopausal obesity by regulating markers of NETs, underscoring the critical role of inflammation in obesity pathogenesis and offering a potential novel therapeutic approach. |
| format | Article |
| id | doaj-art-fffa7345fad14e65bd851586fdccd62e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-fffa7345fad14e65bd851586fdccd62e2025-08-20T04:01:52ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-06379-xAdenosine improves postmenopausal obesity by regulating neutrophil extracellular trapsAnzhu Wang0Wei Zhang1Mengjiao Kang2Shan Wang3Jingjing Shi4Pengfei Xie5Linhua Zhao6Xiaolin Tong7National Center for Integrative Medicine, China-Japan Friendship HospitalSchool of Basic Medicine, Gansu University of Chinese MedicineZhejiang Chinese Medical UniversityNational Center for Integrative Medicine, China-Japan Friendship HospitalThe Second School of Clinical Medicine, Guangzhou University of Chinese MedicineInstitute of Metabolic Diseases, Guang’anmen Hospital, China Academy of Chinese Medical SciencesInstitute of Metabolic Diseases, Guang’anmen Hospital, China Academy of Chinese Medical SciencesDepartment of Endocrinology, The Affiliated Hospital of Changchun University of Chinese MedicineAbstract Postmenopause marks a critical physiological transition in women, triggering changes in fat distribution and promoting obesity, which is strongly linked to systemic low-grade chronic inflammation, with neutrophil extracellular traps (NETs) playing a pivotal role in this process. Differentially expressed genes (DEGs) related to obesity were identified from the GEO database (GSE44000), followed by protein-protein interaction (PPI) network construction and functional enrichment analysis. Immune infiltration analysis was performed to assess changes in the adipose tissue microenvironment. In vivo, a bilateral ovariectomized (OVX) mouse model was employed to evaluate the effects of adenosine on obesity. RT-qPCR and Western blot were used to assess gene expression and NETs markers. A total of 510 DEGs were identified, with 469 upregulated genes primarily linked to inflammation and immune infiltration. PPI analysis highlighted key genes involved in NETs formation. Immune infiltration analysis revealed significant immune microenvironment alterations. Adenosine treatment in OVX mice reduced body weight, fat tissue, liver lipid deposition, and NETs markers (ELANE, MPO), downregulating TNF, ITGB2, and ITGAM. Adenosine ameliorates postmenopausal obesity by regulating markers of NETs, underscoring the critical role of inflammation in obesity pathogenesis and offering a potential novel therapeutic approach.https://doi.org/10.1038/s41598-025-06379-xPostmenopausal obesityAdenosineNeutrophil extracellular trapsInflammatory response |
| spellingShingle | Anzhu Wang Wei Zhang Mengjiao Kang Shan Wang Jingjing Shi Pengfei Xie Linhua Zhao Xiaolin Tong Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps Scientific Reports Postmenopausal obesity Adenosine Neutrophil extracellular traps Inflammatory response |
| title | Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps |
| title_full | Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps |
| title_fullStr | Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps |
| title_full_unstemmed | Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps |
| title_short | Adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps |
| title_sort | adenosine improves postmenopausal obesity by regulating neutrophil extracellular traps |
| topic | Postmenopausal obesity Adenosine Neutrophil extracellular traps Inflammatory response |
| url | https://doi.org/10.1038/s41598-025-06379-x |
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