A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis
Background: Protein kinase D3 (PKD3) is a member of the PKD family that has been implicated in many intracellular signalling pathways. However, defined statements regarding PKD isoform specificity and in vivo functions are rare. Methods: Here, we use PKD3-depleted mouse embryonic fibroblast cells an...
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MDPI AG
2025-02-01
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| Online Access: | https://www.mdpi.com/2227-9059/13/2/345 |
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| author | Ursula Braun Michael Leitges |
| author_facet | Ursula Braun Michael Leitges |
| author_sort | Ursula Braun |
| collection | DOAJ |
| description | Background: Protein kinase D3 (PKD3) is a member of the PKD family that has been implicated in many intracellular signalling pathways. However, defined statements regarding PKD isoform specificity and in vivo functions are rare. Methods: Here, we use PKD3-depleted mouse embryonic fibroblast cells and employ various cell culture-based assays and fluorescence microscopy. Results: We show that PKD3 is involved in the regulation of cytokinesis after immortalisation by modulating RhoA activity through a PKCε/PKD3 signalling axis. Conclusions: PKD3 depletion leads to prolonged RhoA activity during cytokinesis, resulting in failed abscission and an increase in the number of multinucleated cells. This identifies a novel, previously unrecognised PKCε/PKD3 pathway involved in the modulation of cytokinesis. |
| format | Article |
| id | doaj-art-fff902ae8dcc40e5b14be9e3ccbbb89b |
| institution | DOAJ |
| issn | 2227-9059 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | MDPI AG |
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| series | Biomedicines |
| spelling | doaj-art-fff902ae8dcc40e5b14be9e3ccbbb89b2025-08-20T03:12:10ZengMDPI AGBiomedicines2227-90592025-02-0113234510.3390/biomedicines13020345A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During CytokinesisUrsula Braun0Michael Leitges1Division of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, 300 Prince Philip Drive, St. John’s, NL A1B 3V6, CanadaDivision of BioMedical Sciences, Faculty of Medicine, Memorial University of Newfoundland, 300 Prince Philip Drive, St. John’s, NL A1B 3V6, CanadaBackground: Protein kinase D3 (PKD3) is a member of the PKD family that has been implicated in many intracellular signalling pathways. However, defined statements regarding PKD isoform specificity and in vivo functions are rare. Methods: Here, we use PKD3-depleted mouse embryonic fibroblast cells and employ various cell culture-based assays and fluorescence microscopy. Results: We show that PKD3 is involved in the regulation of cytokinesis after immortalisation by modulating RhoA activity through a PKCε/PKD3 signalling axis. Conclusions: PKD3 depletion leads to prolonged RhoA activity during cytokinesis, resulting in failed abscission and an increase in the number of multinucleated cells. This identifies a novel, previously unrecognised PKCε/PKD3 pathway involved in the modulation of cytokinesis.https://www.mdpi.com/2227-9059/13/2/345protein kinase D3protein kinase CεRhoAgene targetingmouse embryonic fibroblastscytokinesis |
| spellingShingle | Ursula Braun Michael Leitges A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis Biomedicines protein kinase D3 protein kinase Cε RhoA gene targeting mouse embryonic fibroblasts cytokinesis |
| title | A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis |
| title_full | A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis |
| title_fullStr | A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis |
| title_full_unstemmed | A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis |
| title_short | A Protein Kinase Cε/Protein Kinase D3 Signalling Axis Modulates RhoA Activity During Cytokinesis |
| title_sort | protein kinase cε protein kinase d3 signalling axis modulates rhoa activity during cytokinesis |
| topic | protein kinase D3 protein kinase Cε RhoA gene targeting mouse embryonic fibroblasts cytokinesis |
| url | https://www.mdpi.com/2227-9059/13/2/345 |
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