The Role of Long Non-Coding RNAs in Human Endoderm Differentiation

The human genome sequencing revealed a vast complexity of transcripts, with over 80% of the genome being transcribed into non-coding RNAs. In particular, long non-coding RNAs (lncRNAs) have emerged as critical regulators of various cellular processes, including embryonic development and stem cell di...

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Main Authors: Annanda Lyra Ribeiro, Bruno Dallagiovanna
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Non-Coding RNA
Subjects:
Online Access:https://www.mdpi.com/2311-553X/11/2/29
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author Annanda Lyra Ribeiro
Bruno Dallagiovanna
author_facet Annanda Lyra Ribeiro
Bruno Dallagiovanna
author_sort Annanda Lyra Ribeiro
collection DOAJ
description The human genome sequencing revealed a vast complexity of transcripts, with over 80% of the genome being transcribed into non-coding RNAs. In particular, long non-coding RNAs (lncRNAs) have emerged as critical regulators of various cellular processes, including embryonic development and stem cell differentiation. Despite extensive efforts to identify and characterize lncRNAs, defining their mechanisms of action in state-specific cellular contexts remains a significant challenge. Only recently has the involvement of lncRNAs in human endoderm differentiation of pluripotent stem cells begun to be addressed, creating an opportunity to explore the mechanisms by which lncRNAs exert their functions in germ layer formation, lineage specification, and commitment. This review summarizes current findings on the roles of lncRNAs in endoderm differentiation, highlighting the functional mechanisms and regulatory aspects underlying their involvement in cell fate decisions leading to endoderm development. The key lncRNAs implicated in endoderm differentiation are discussed, along with their interaction with transcription factors and RNA-binding proteins and modulation of signaling pathways essential for endoderm development. Gaining insight into the regulatory roles of lncRNAs in endoderm differentiation enhances the understanding of developmental biology and provides a foundation for discovering novel lncRNAs involved in cell fate determination.
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spelling doaj-art-ffe50652742f4dc481f8ccc6ebb0420a2025-08-20T02:18:20ZengMDPI AGNon-Coding RNA2311-553X2025-04-011122910.3390/ncrna11020029The Role of Long Non-Coding RNAs in Human Endoderm DifferentiationAnnanda Lyra Ribeiro0Bruno Dallagiovanna1Stem Cells Basic Biology Laboratory, Carlos Chagas Institute—FIOCRUZ/PR, Curitiba 81350-010, BrazilStem Cells Basic Biology Laboratory, Carlos Chagas Institute—FIOCRUZ/PR, Curitiba 81350-010, BrazilThe human genome sequencing revealed a vast complexity of transcripts, with over 80% of the genome being transcribed into non-coding RNAs. In particular, long non-coding RNAs (lncRNAs) have emerged as critical regulators of various cellular processes, including embryonic development and stem cell differentiation. Despite extensive efforts to identify and characterize lncRNAs, defining their mechanisms of action in state-specific cellular contexts remains a significant challenge. Only recently has the involvement of lncRNAs in human endoderm differentiation of pluripotent stem cells begun to be addressed, creating an opportunity to explore the mechanisms by which lncRNAs exert their functions in germ layer formation, lineage specification, and commitment. This review summarizes current findings on the roles of lncRNAs in endoderm differentiation, highlighting the functional mechanisms and regulatory aspects underlying their involvement in cell fate decisions leading to endoderm development. The key lncRNAs implicated in endoderm differentiation are discussed, along with their interaction with transcription factors and RNA-binding proteins and modulation of signaling pathways essential for endoderm development. Gaining insight into the regulatory roles of lncRNAs in endoderm differentiation enhances the understanding of developmental biology and provides a foundation for discovering novel lncRNAs involved in cell fate determination.https://www.mdpi.com/2311-553X/11/2/29lncRNAshuman endoderm differentiationstem cells
spellingShingle Annanda Lyra Ribeiro
Bruno Dallagiovanna
The Role of Long Non-Coding RNAs in Human Endoderm Differentiation
Non-Coding RNA
lncRNAs
human endoderm differentiation
stem cells
title The Role of Long Non-Coding RNAs in Human Endoderm Differentiation
title_full The Role of Long Non-Coding RNAs in Human Endoderm Differentiation
title_fullStr The Role of Long Non-Coding RNAs in Human Endoderm Differentiation
title_full_unstemmed The Role of Long Non-Coding RNAs in Human Endoderm Differentiation
title_short The Role of Long Non-Coding RNAs in Human Endoderm Differentiation
title_sort role of long non coding rnas in human endoderm differentiation
topic lncRNAs
human endoderm differentiation
stem cells
url https://www.mdpi.com/2311-553X/11/2/29
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