Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua
Polygonatum cyrtonema Hua, a medicinal herb valued in traditional Chinese medicine, produces bioactive polysaccharides and saponins, exhibits intraspecific metabolic variations whose interactions with rhizosphere microbiota remain unexplored. As a pilot investigation, we characterized these linkages...
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1615900/full |
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| author | Fang Liu Wenlong Dong Yi An Hongyu Qian Chunlin Gui Chunlin Gui Yunjian Xu |
| author_facet | Fang Liu Wenlong Dong Yi An Hongyu Qian Chunlin Gui Chunlin Gui Yunjian Xu |
| author_sort | Fang Liu |
| collection | DOAJ |
| description | Polygonatum cyrtonema Hua, a medicinal herb valued in traditional Chinese medicine, produces bioactive polysaccharides and saponins, exhibits intraspecific metabolic variations whose interactions with rhizosphere microbiota remain unexplored. As a pilot investigation, we characterized these linkages in two representative high-yield cultivars (JH1: bead-like tubers; JH2: L-shaped rhizomes) through integrated 16S rRNA/ITS sequencing and metabolomics. Bacterial communities showed conserved composition (Proteobacteria-dominated; shared core genera Candidatus Koribacter and Bradyrhizobium), whereas fungal assemblages diverged sharply between cultivars. JH1 rhizospheres enriched Hydnum, Collimyces, Ramariopsis and Coralloidiomyces, whereas JH2 favored Acremonium, Archaeospora, Didymosphaeria, Entoloma and Monacrosporium. Metabolomic profiling revealed tissue-driven specialization as the primary determinant, with tubers accumulated oleoyl ethylamide/DL-malic acid and roots preferentially storing DL-arginine. The core bacteria exhibited consistent negative association with organ-specific metabolites, whereas, fungal interactions diverged. JH1 enriched fungi positively associated with tuber oleoyl ethylamide and root DL-arginine but negatively with tuber DL-malic acid, while JH2-enriched taxa showed inverse relationships. The enriched fungal communities (average positive correlation coefficient 0.39) demonstrated stronger tissue-specific metabolite coordination than bacteria (average positive correlation coefficient 0.15), suggesting potential mycobiome-mediated regulation of medicinal compound partitioning. This preliminary dissection of cultivar-associated microbial-metabolite interplay may provide a mechanistic framework for optimizing P. cyrtonema cultivation through synthetic microbial consortia. However, future multi-location, multi-season studies with soil controls are needed to validate ecological generality. |
| format | Article |
| id | doaj-art-ffe180748ca44a9ca68823bbe5ca1048 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-ffe180748ca44a9ca68823bbe5ca10482025-08-20T03:25:27ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-07-011610.3389/fmicb.2025.16159001615900Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema HuaFang Liu0Wenlong Dong1Yi An2Hongyu Qian3Chunlin Gui4Chunlin Gui5Yunjian Xu6School of Agriculture, Yunnan University, Kunming, ChinaSchool of Agriculture, Yunnan University, Kunming, ChinaSchool of Agriculture, Yunnan University, Kunming, ChinaSchool of Agriculture, Yunnan University, Kunming, ChinaChizhou Rural Revitalization Industry Development Co., Ltd., Chizhou, ChinaAnhui Jinyunong Biotechnology Co., Ltd., Chizhou, ChinaState Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Ministry of Education Key Laboratory for Transboundary Ecosecurity of Southwest China, and Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology, School of Ecology and Environmental Science, Institute of Biodiversity, Yunnan University, Kunming, ChinaPolygonatum cyrtonema Hua, a medicinal herb valued in traditional Chinese medicine, produces bioactive polysaccharides and saponins, exhibits intraspecific metabolic variations whose interactions with rhizosphere microbiota remain unexplored. As a pilot investigation, we characterized these linkages in two representative high-yield cultivars (JH1: bead-like tubers; JH2: L-shaped rhizomes) through integrated 16S rRNA/ITS sequencing and metabolomics. Bacterial communities showed conserved composition (Proteobacteria-dominated; shared core genera Candidatus Koribacter and Bradyrhizobium), whereas fungal assemblages diverged sharply between cultivars. JH1 rhizospheres enriched Hydnum, Collimyces, Ramariopsis and Coralloidiomyces, whereas JH2 favored Acremonium, Archaeospora, Didymosphaeria, Entoloma and Monacrosporium. Metabolomic profiling revealed tissue-driven specialization as the primary determinant, with tubers accumulated oleoyl ethylamide/DL-malic acid and roots preferentially storing DL-arginine. The core bacteria exhibited consistent negative association with organ-specific metabolites, whereas, fungal interactions diverged. JH1 enriched fungi positively associated with tuber oleoyl ethylamide and root DL-arginine but negatively with tuber DL-malic acid, while JH2-enriched taxa showed inverse relationships. The enriched fungal communities (average positive correlation coefficient 0.39) demonstrated stronger tissue-specific metabolite coordination than bacteria (average positive correlation coefficient 0.15), suggesting potential mycobiome-mediated regulation of medicinal compound partitioning. This preliminary dissection of cultivar-associated microbial-metabolite interplay may provide a mechanistic framework for optimizing P. cyrtonema cultivation through synthetic microbial consortia. However, future multi-location, multi-season studies with soil controls are needed to validate ecological generality.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1615900/fullPolygonatum cyrtonema Huarhizosphere microbiotafungal communitiesmetabolitesmicrobial-metabolite interactions |
| spellingShingle | Fang Liu Wenlong Dong Yi An Hongyu Qian Chunlin Gui Chunlin Gui Yunjian Xu Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua Frontiers in Microbiology Polygonatum cyrtonema Hua rhizosphere microbiota fungal communities metabolites microbial-metabolite interactions |
| title | Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua |
| title_full | Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua |
| title_fullStr | Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua |
| title_full_unstemmed | Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua |
| title_short | Pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of Polygonatum cyrtonema Hua |
| title_sort | pilot investigation of the rhizosphere microbial communities and metabolism of two cultivars of polygonatum cyrtonema hua |
| topic | Polygonatum cyrtonema Hua rhizosphere microbiota fungal communities metabolites microbial-metabolite interactions |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1615900/full |
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