Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages
Here, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflam...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2010/475139 |
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| author | Christian R. Gomez John Karavitis Jessica L. Palmer Douglas E. Faunce Luis Ramirez Vanessa Nomellini Elizabeth J. Kovacs |
| author_facet | Christian R. Gomez John Karavitis Jessica L. Palmer Douglas E. Faunce Luis Ramirez Vanessa Nomellini Elizabeth J. Kovacs |
| author_sort | Christian R. Gomez |
| collection | DOAJ |
| description | Here, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflammatory cytokines. In contrast, when compared to macrophages from young IL-6 KO mice, LPS stimulation yielded higher levels of these cytokines by cells from aged IL-6 KO mice. Aging or IL-6 deficiency did not affected the percentage of F4/80+ macrophages, or the surface expression of Toll-like receptor 4 (TLR4) and components of the IL-6 receptor. Overall, our results indicate that IL-6 plays a role in regulating the age-related defects in macrophages through alteration of proinflammatory cytokines, adding to the complexity of IL-6-mediated impairment of immune cell function with increasing age. |
| format | Article |
| id | doaj-art-ffd3e5ae7cea46efafafc23cd0112440 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-ffd3e5ae7cea46efafafc23cd01124402025-08-20T03:25:54ZengWileyMediators of Inflammation0962-93511466-18612010-01-01201010.1155/2010/475139475139Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by MacrophagesChristian R. Gomez0John Karavitis1Jessica L. Palmer2Douglas E. Faunce3Luis Ramirez4Vanessa Nomellini5Elizabeth J. Kovacs6Department of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USADepartment of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USADepartment of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USADepartment of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USADepartment of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USADepartment of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USADepartment of Surgery, and Immunology and Aging Program, The Burn and Shock Trauma Institute, Loyola University Medical Center, 2160 South First Avenue, Maywood, IL 60153, USAHere, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflammatory cytokines. In contrast, when compared to macrophages from young IL-6 KO mice, LPS stimulation yielded higher levels of these cytokines by cells from aged IL-6 KO mice. Aging or IL-6 deficiency did not affected the percentage of F4/80+ macrophages, or the surface expression of Toll-like receptor 4 (TLR4) and components of the IL-6 receptor. Overall, our results indicate that IL-6 plays a role in regulating the age-related defects in macrophages through alteration of proinflammatory cytokines, adding to the complexity of IL-6-mediated impairment of immune cell function with increasing age.http://dx.doi.org/10.1155/2010/475139 |
| spellingShingle | Christian R. Gomez John Karavitis Jessica L. Palmer Douglas E. Faunce Luis Ramirez Vanessa Nomellini Elizabeth J. Kovacs Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages Mediators of Inflammation |
| title | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
| title_full | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
| title_fullStr | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
| title_full_unstemmed | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
| title_short | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
| title_sort | interleukin 6 contributes to age related alteration of cytokine production by macrophages |
| url | http://dx.doi.org/10.1155/2010/475139 |
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