Mutagenesis Targeting the S<sup>153</sup> Residue Within the Transmembrane β-Hairpin of Mosquito-Larvicidal Mpp46Ab Affects Its Toxicity and the Synergistic Toxicity with Cry4Aa

Mutagenesis Targeting the S<sup>153</sup> Residue Within the Transmembrane β-Hairpin of Mosquito-Larvicidal Mpp46Ab Affects Its Toxicity and the Synergistic Toxicity with Cry4Aa

We constructed a library of Mpp46Ab mutants, in which S<sup>153</sup> within the transmembrane β-hairpin was randomly replaced by other amino acids. Mutagenesis and subsequent primary screening yielded 10 different Mpp46Ab mutants in addition to the wild type. Remarkably, S<sup>153...

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Bibliographic Details
Main Authors: Tohru Hayakawa, Syun Yamaoka, Mami Asakura, Minako Hirano, Toru Ide
Format: Article
Language:English
Published: MDPI AG 2025-04-01
Series:Biology
Subjects:
<i>Bacillus thuringiensis</i>
mosquito-larvicidal proteins
synergistic toxicity
<i>Culex pipiens</i> mosquito larvae
side-directed mutagenesis
electrophysiologic analysis
Online Access:https://www.mdpi.com/2079-7737/14/5/489
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