Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors.
Gene expression in eukaryotes is controlled by DNA sequences at promoter and enhancer regions, whose accessibility for binding by regulatory proteins dictates their specific patterns of activity. Here, we identify the protein Zbtb7a as a factor required for inducible changes in accessibility driven...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-05-01
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| Series: | PLoS Biology |
| Online Access: | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.2004526&type=printable |
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| _version_ | 1849722152395210752 |
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| author | José Miguel Ramos Pittol Agata Oruba Gerhard Mittler Simona Saccani Dominic van Essen |
| author_facet | José Miguel Ramos Pittol Agata Oruba Gerhard Mittler Simona Saccani Dominic van Essen |
| author_sort | José Miguel Ramos Pittol |
| collection | DOAJ |
| description | Gene expression in eukaryotes is controlled by DNA sequences at promoter and enhancer regions, whose accessibility for binding by regulatory proteins dictates their specific patterns of activity. Here, we identify the protein Zbtb7a as a factor required for inducible changes in accessibility driven by transcription factors (TFs). We show that Zbtb7a binds to a significant fraction of genomic promoters and enhancers, encompassing many target genes of nuclear factor kappa B (NFκB) p65 and a variety of other TFs. While Zbtb7a binding is not alone sufficient to directly activate promoters, it is required to enable TF-dependent control of accessibility and normal gene expression. Using p65 as a model TF, we show that Zbtb7a associates with promoters independently of client TF binding. Moreover, the presence of prebound Zbtb7a can specify promoters that are amenable to TF-induced changes in accessibility. Therefore, Zbtb7a represents a widely used promoter factor that transduces signals from other TFs to enable control of accessibility and regulation of gene expression. |
| format | Article |
| id | doaj-art-ffaf8ac271ed4b99b59617a76da1835a |
| institution | DOAJ |
| issn | 1544-9173 1545-7885 |
| language | English |
| publishDate | 2018-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Biology |
| spelling | doaj-art-ffaf8ac271ed4b99b59617a76da1835a2025-08-20T03:11:25ZengPublic Library of Science (PLoS)PLoS Biology1544-91731545-78852018-05-01165e200452610.1371/journal.pbio.2004526Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors.José Miguel Ramos PittolAgata OrubaGerhard MittlerSimona SaccaniDominic van EssenGene expression in eukaryotes is controlled by DNA sequences at promoter and enhancer regions, whose accessibility for binding by regulatory proteins dictates their specific patterns of activity. Here, we identify the protein Zbtb7a as a factor required for inducible changes in accessibility driven by transcription factors (TFs). We show that Zbtb7a binds to a significant fraction of genomic promoters and enhancers, encompassing many target genes of nuclear factor kappa B (NFκB) p65 and a variety of other TFs. While Zbtb7a binding is not alone sufficient to directly activate promoters, it is required to enable TF-dependent control of accessibility and normal gene expression. Using p65 as a model TF, we show that Zbtb7a associates with promoters independently of client TF binding. Moreover, the presence of prebound Zbtb7a can specify promoters that are amenable to TF-induced changes in accessibility. Therefore, Zbtb7a represents a widely used promoter factor that transduces signals from other TFs to enable control of accessibility and regulation of gene expression.https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.2004526&type=printable |
| spellingShingle | José Miguel Ramos Pittol Agata Oruba Gerhard Mittler Simona Saccani Dominic van Essen Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors. PLoS Biology |
| title | Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors. |
| title_full | Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors. |
| title_fullStr | Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors. |
| title_full_unstemmed | Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors. |
| title_short | Zbtb7a is a transducer for the control of promoter accessibility by NF-kappa B and multiple other transcription factors. |
| title_sort | zbtb7a is a transducer for the control of promoter accessibility by nf kappa b and multiple other transcription factors |
| url | https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.2004526&type=printable |
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