Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa
The presence of antibiotic residues in the environment raises concerns about toxicity to aquatic organisms and the emergence and development of antibiotic-resistant bacteria. This experimental study investigated the removal efficiency of the antibiotics cephalexin and ceftriaxone from synthetic wast...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-07-01
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| Series: | Desalination and Water Treatment |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1944398625003935 |
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| author | Mohammad Javad Shokoohizadeh Seyyed Alireza Mousavi Samira Khamutian Ali Almasi |
| author_facet | Mohammad Javad Shokoohizadeh Seyyed Alireza Mousavi Samira Khamutian Ali Almasi |
| author_sort | Mohammad Javad Shokoohizadeh |
| collection | DOAJ |
| description | The presence of antibiotic residues in the environment raises concerns about toxicity to aquatic organisms and the emergence and development of antibiotic-resistant bacteria. This experimental study investigated the removal efficiency of the antibiotics cephalexin and ceftriaxone from synthetic wastewater using bioaugmentation in a laboratory-scale moving bed biofilm reactor. The bioaugmentation was performed with metallo- β-lactamase-producing Pseudomonas aeruginosa to enhance antibiotic degradation. The study examined the effects of two key factors: initial antibiotic concentrations (15, 30, and 45 mg/L) and hydraulic retention times (HRT) of 4, 8, and 12 h (hr). Results showed that bioaugmentation significantly improved the removal efficiency of both antibiotics. The highest removal rates were achieved at an HRT of 12 hr and an antibiotic concentration of 30 mg/L, with 90 % removal for cephalexin and 73 % for ceftriaxone. These findings suggest that bioaugmentation of MBBR with metallo-β-lactamase-producing P. aeruginosa is an effective and economical method for eliminating β-lactam antibiotics from contaminated wastewaters, such as those from pharmaceutical and hospital sources. This approach offers a promising solution to reduce antibiotic residues in the environment, thereby mitigating antibiotic potency in aquatic environments and the spread of antibiotic-resistant bacteria. |
| format | Article |
| id | doaj-art-ffab73e7fb3447a493974635c3f9e92a |
| institution | Kabale University |
| issn | 1944-3986 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Desalination and Water Treatment |
| spelling | doaj-art-ffab73e7fb3447a493974635c3f9e92a2025-08-22T04:56:02ZengElsevierDesalination and Water Treatment1944-39862025-07-0132310137710.1016/j.dwt.2025.101377Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosaMohammad Javad Shokoohizadeh0Seyyed Alireza Mousavi1Samira Khamutian2Ali Almasi3Department of Environmental Health Engineering, School of Public Health, Kermanshah University of Medical Sciences, Kermanshah, Iran; Social Development and Health Promotion Research Center, Health Policy and Promotion Institute, Kermanshah University of Medical Sciences, Kermanshah, IranHealth, Safety and Environment Technologies Research Core, Health Technology Institute, Kermanshah, Department of Environmental Health Engineering, School of Public Health, Kermanshah, IranSocial Development and Health Promotion Research Center, Health Policy and Promotion Institute, Kermanshah University of Medical Sciences, Kermanshah, IranSocial Development and Health Promotion Research Center, Health Policy and Promotion Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran; Corresponding author.The presence of antibiotic residues in the environment raises concerns about toxicity to aquatic organisms and the emergence and development of antibiotic-resistant bacteria. This experimental study investigated the removal efficiency of the antibiotics cephalexin and ceftriaxone from synthetic wastewater using bioaugmentation in a laboratory-scale moving bed biofilm reactor. The bioaugmentation was performed with metallo- β-lactamase-producing Pseudomonas aeruginosa to enhance antibiotic degradation. The study examined the effects of two key factors: initial antibiotic concentrations (15, 30, and 45 mg/L) and hydraulic retention times (HRT) of 4, 8, and 12 h (hr). Results showed that bioaugmentation significantly improved the removal efficiency of both antibiotics. The highest removal rates were achieved at an HRT of 12 hr and an antibiotic concentration of 30 mg/L, with 90 % removal for cephalexin and 73 % for ceftriaxone. These findings suggest that bioaugmentation of MBBR with metallo-β-lactamase-producing P. aeruginosa is an effective and economical method for eliminating β-lactam antibiotics from contaminated wastewaters, such as those from pharmaceutical and hospital sources. This approach offers a promising solution to reduce antibiotic residues in the environment, thereby mitigating antibiotic potency in aquatic environments and the spread of antibiotic-resistant bacteria.http://www.sciencedirect.com/science/article/pii/S1944398625003935BioaugmentationWastewaterβ-lactam antibioticsMetallo β-lactamasePseudomonas aeruginosa |
| spellingShingle | Mohammad Javad Shokoohizadeh Seyyed Alireza Mousavi Samira Khamutian Ali Almasi Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa Desalination and Water Treatment Bioaugmentation Wastewater β-lactam antibiotics Metallo β-lactamase Pseudomonas aeruginosa |
| title | Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa |
| title_full | Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa |
| title_fullStr | Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa |
| title_full_unstemmed | Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa |
| title_short | Enhanced removal of β-lactam antibiotics from synthetic wastewater via bioaugmentation of MBBR by MBL-positive Pseudomonas aeruginosa |
| title_sort | enhanced removal of β lactam antibiotics from synthetic wastewater via bioaugmentation of mbbr by mbl positive pseudomonas aeruginosa |
| topic | Bioaugmentation Wastewater β-lactam antibiotics Metallo β-lactamase Pseudomonas aeruginosa |
| url | http://www.sciencedirect.com/science/article/pii/S1944398625003935 |
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