Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer
BackgroundProstate cancer (PCa), a highly heterogeneous cancer with a complex molecular pathogenesis, is a leading cause of cancer-related mortality among men globally. The present study presents a lipid metabolism-based risk model for PCa and explores the role of succinyl-CoA ligase GDP-forming sub...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-07-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2025.1592779/full |
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| author | Bao Hua Qing Yang Shangqing Song Wenfeng Li Bin Xu Yufei Gu |
| author_facet | Bao Hua Qing Yang Shangqing Song Wenfeng Li Bin Xu Yufei Gu |
| author_sort | Bao Hua |
| collection | DOAJ |
| description | BackgroundProstate cancer (PCa), a highly heterogeneous cancer with a complex molecular pathogenesis, is a leading cause of cancer-related mortality among men globally. The present study presents a lipid metabolism-based risk model for PCa and explores the role of succinyl-CoA ligase GDP-forming subunit beta (SUCLG2), a potential marker and therapeutic target in PCa involved in lipid metabolism and cancer progression, from the perspective of developing effective diagnostic and therapeutic strategies.MethodsHigh-throughput RNA sequencing and single-cell RNA sequencing were used to investigate the expression and functional relevance of SUCLG2 in PCa. We analyzed 497 PCa samples from The Cancer Genome Atlas and conducted a comprehensive bioinformatics analysis, including univariate Cox proportional hazards regression, least absolute shrinkage and selection operator regression, and gene set enrichment analysis. Furthermore, quantitative real-time polymerase chain reaction and immunofluorescence assays were performed to validate SUCLG2 expression in clinical samples and the prostate carcinoma epithelial cell line 22Rv1.ResultsOur findings revealed that lipid metabolism-related genes, including SUCLG2, have significant prognostic value, based on a 16-gene risk model constructed that accurately predicted PCa prognosis. In particular, SUCLG2 was significantly enriched in luminal and basal/intermediate cell subsets, highlighting its potential role in tumor progression and therapy resistance. Drug sensitivity analysis indicated that SUCLG2 expression is correlated with the efficacy of several chemotherapeutic agents, based on which strategies for personalized therapy in PCa treatment could be devised.ConclusionSUCLG2 plays a pivotal role in the metabolic reprogramming of PCa, thus offering new insights into its progression and potential therapeutic targets. Our study underscores the importance of metabolic pathways in PCa pathogenesis and paves the way for the development of targeted therapies, thus contributing to personalized medicine in PCa management. |
| format | Article |
| id | doaj-art-ffa6738c272e4874b6ffecd49778ce38 |
| institution | Kabale University |
| issn | 1664-8021 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj-art-ffa6738c272e4874b6ffecd49778ce382025-08-20T03:50:58ZengFrontiers Media S.A.Frontiers in Genetics1664-80212025-07-011610.3389/fgene.2025.15927791592779Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancerBao HuaQing YangShangqing SongWenfeng LiBin XuYufei GuBackgroundProstate cancer (PCa), a highly heterogeneous cancer with a complex molecular pathogenesis, is a leading cause of cancer-related mortality among men globally. The present study presents a lipid metabolism-based risk model for PCa and explores the role of succinyl-CoA ligase GDP-forming subunit beta (SUCLG2), a potential marker and therapeutic target in PCa involved in lipid metabolism and cancer progression, from the perspective of developing effective diagnostic and therapeutic strategies.MethodsHigh-throughput RNA sequencing and single-cell RNA sequencing were used to investigate the expression and functional relevance of SUCLG2 in PCa. We analyzed 497 PCa samples from The Cancer Genome Atlas and conducted a comprehensive bioinformatics analysis, including univariate Cox proportional hazards regression, least absolute shrinkage and selection operator regression, and gene set enrichment analysis. Furthermore, quantitative real-time polymerase chain reaction and immunofluorescence assays were performed to validate SUCLG2 expression in clinical samples and the prostate carcinoma epithelial cell line 22Rv1.ResultsOur findings revealed that lipid metabolism-related genes, including SUCLG2, have significant prognostic value, based on a 16-gene risk model constructed that accurately predicted PCa prognosis. In particular, SUCLG2 was significantly enriched in luminal and basal/intermediate cell subsets, highlighting its potential role in tumor progression and therapy resistance. Drug sensitivity analysis indicated that SUCLG2 expression is correlated with the efficacy of several chemotherapeutic agents, based on which strategies for personalized therapy in PCa treatment could be devised.ConclusionSUCLG2 plays a pivotal role in the metabolic reprogramming of PCa, thus offering new insights into its progression and potential therapeutic targets. Our study underscores the importance of metabolic pathways in PCa pathogenesis and paves the way for the development of targeted therapies, thus contributing to personalized medicine in PCa management.https://www.frontiersin.org/articles/10.3389/fgene.2025.1592779/fullprostate cancerSUCLG2lipid metabolismsingle-cell RNA sequencingpersonalized therapy |
| spellingShingle | Bao Hua Qing Yang Shangqing Song Wenfeng Li Bin Xu Yufei Gu Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer Frontiers in Genetics prostate cancer SUCLG2 lipid metabolism single-cell RNA sequencing personalized therapy |
| title | Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer |
| title_full | Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer |
| title_fullStr | Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer |
| title_full_unstemmed | Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer |
| title_short | Exploring the prognostic significance and therapeutic potential of SUCLG2 in prostate cancer |
| title_sort | exploring the prognostic significance and therapeutic potential of suclg2 in prostate cancer |
| topic | prostate cancer SUCLG2 lipid metabolism single-cell RNA sequencing personalized therapy |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2025.1592779/full |
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