Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease
This study aimed to explore the effect of cell division cycle protein 42 (CDC42) on inflammatory response and immune response in mice bearing inflammatory bowel disease (IBD). Trinitrobenzene sulfonic acid was injected into the colon of mice to establish IBD model. The mice were divided into four gr...
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1596936 |
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| author | Le-Mei Dong Xiao-Wei Chen Xi-Xi He Xue-Pei Jiang Fang Wu |
| author_facet | Le-Mei Dong Xiao-Wei Chen Xi-Xi He Xue-Pei Jiang Fang Wu |
| author_sort | Le-Mei Dong |
| collection | DOAJ |
| description | This study aimed to explore the effect of cell division cycle protein 42 (CDC42) on inflammatory response and immune response in mice bearing inflammatory bowel disease (IBD). Trinitrobenzene sulfonic acid was injected into the colon of mice to establish IBD model. The mice were divided into four groups (n = 4): control, model, Ad5, and Ad5-CDC42. After establishing IBD model, mice which were treated with AD5 empty vector and AD5-CDC42 expression vector served as the Ad5 group and Ad5-CDC42 group, respectively. The mRNA and protein levels of interleukin 10 (IL-10), interferon-γ (IFN-γ), IL-4, and tumor necrosis factor-α (TNF-α) in the colon tissues were evaluated by RT-PCR and western blot, respectively. Their levels in the serum and colon tissues were examined by ELISA assay and immunohistochemical analysis, respectively. Their changes in the mRNA and protein levels were consistent and similar changes in the colon tissues and the serum were found among various groups. The levels of IL-10, IFN-γ, IL-4, and TNF-α were lowest in the control group. Their levels in the model group and the Ad5 group were similar (p > .05) and significantly higher than those in the control group (p < .05). In comparison with the model group and the Ad5 group, their levels were significantly reduced in the Ad5-CDC42 group (p < .05). In conclusion, the levels of inflammatory cytokines were elevated in the colon tissues and serum of IBD mice, which could be reduced by the CDC42 treatment. CDC42 regulated the inflammatory response and the innate immune response in IBD mice. |
| format | Article |
| id | doaj-art-ff9db3f71cc046d0843e26664ee5a54d |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
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| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-ff9db3f71cc046d0843e26664ee5a54d2025-08-20T03:51:25ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014711833183810.1080/21691401.2019.1596936Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel diseaseLe-Mei Dong0Xiao-Wei Chen1Xi-Xi He2Xue-Pei Jiang3Fang Wu4Department of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaDepartment of Gastroenterology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, ChinaThis study aimed to explore the effect of cell division cycle protein 42 (CDC42) on inflammatory response and immune response in mice bearing inflammatory bowel disease (IBD). Trinitrobenzene sulfonic acid was injected into the colon of mice to establish IBD model. The mice were divided into four groups (n = 4): control, model, Ad5, and Ad5-CDC42. After establishing IBD model, mice which were treated with AD5 empty vector and AD5-CDC42 expression vector served as the Ad5 group and Ad5-CDC42 group, respectively. The mRNA and protein levels of interleukin 10 (IL-10), interferon-γ (IFN-γ), IL-4, and tumor necrosis factor-α (TNF-α) in the colon tissues were evaluated by RT-PCR and western blot, respectively. Their levels in the serum and colon tissues were examined by ELISA assay and immunohistochemical analysis, respectively. Their changes in the mRNA and protein levels were consistent and similar changes in the colon tissues and the serum were found among various groups. The levels of IL-10, IFN-γ, IL-4, and TNF-α were lowest in the control group. Their levels in the model group and the Ad5 group were similar (p > .05) and significantly higher than those in the control group (p < .05). In comparison with the model group and the Ad5 group, their levels were significantly reduced in the Ad5-CDC42 group (p < .05). In conclusion, the levels of inflammatory cytokines were elevated in the colon tissues and serum of IBD mice, which could be reduced by the CDC42 treatment. CDC42 regulated the inflammatory response and the innate immune response in IBD mice.https://www.tandfonline.com/doi/10.1080/21691401.2019.1596936Cell division cycle protein 42inflammatory bowel diseaseinflammatory responseinnate immune responsecolonic inflammation |
| spellingShingle | Le-Mei Dong Xiao-Wei Chen Xi-Xi He Xue-Pei Jiang Fang Wu Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease Artificial Cells, Nanomedicine, and Biotechnology Cell division cycle protein 42 inflammatory bowel disease inflammatory response innate immune response colonic inflammation |
| title | Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease |
| title_full | Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease |
| title_fullStr | Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease |
| title_full_unstemmed | Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease |
| title_short | Cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease |
| title_sort | cell division cycle protein 42 regulates the inflammatory response in mice bearing inflammatory bowel disease |
| topic | Cell division cycle protein 42 inflammatory bowel disease inflammatory response innate immune response colonic inflammation |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1596936 |
| work_keys_str_mv | AT lemeidong celldivisioncycleprotein42regulatestheinflammatoryresponseinmicebearinginflammatoryboweldisease AT xiaoweichen celldivisioncycleprotein42regulatestheinflammatoryresponseinmicebearinginflammatoryboweldisease AT xixihe celldivisioncycleprotein42regulatestheinflammatoryresponseinmicebearinginflammatoryboweldisease AT xuepeijiang celldivisioncycleprotein42regulatestheinflammatoryresponseinmicebearinginflammatoryboweldisease AT fangwu celldivisioncycleprotein42regulatestheinflammatoryresponseinmicebearinginflammatoryboweldisease |