Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process

In this study, an artificial intelligence-based machine vision system was developed for in-line particle size analysis during the pellet layering process. Drug-layered pellets were produced by coating microcrystalline cellulose cores with an ibuprofen-containing layering liquid until the target drug...

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Main Authors: Orsolya Péterfi, Nikolett Kállai-Szabó, Kincső Renáta Demeter, Ádám Tibor Barna, István Antal, Edina Szabó, Emese Sipos, Zsombor Kristóf Nagy, Dorián László Galata
Format: Article
Language:English
Published: Elsevier 2025-08-01
Series:Journal of Pharmaceutical Analysis
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Online Access:http://www.sciencedirect.com/science/article/pii/S2095177925000449
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author Orsolya Péterfi
Nikolett Kállai-Szabó
Kincső Renáta Demeter
Ádám Tibor Barna
István Antal
Edina Szabó
Emese Sipos
Zsombor Kristóf Nagy
Dorián László Galata
author_facet Orsolya Péterfi
Nikolett Kállai-Szabó
Kincső Renáta Demeter
Ádám Tibor Barna
István Antal
Edina Szabó
Emese Sipos
Zsombor Kristóf Nagy
Dorián László Galata
author_sort Orsolya Péterfi
collection DOAJ
description In this study, an artificial intelligence-based machine vision system was developed for in-line particle size analysis during the pellet layering process. Drug-layered pellets were produced by coating microcrystalline cellulose cores with an ibuprofen-containing layering liquid until the target drug content was achieved. Drug content increases with pellet size; therefore, particle size monitoring can ensure product safety and quality. The direct imaging system, consisting of a rigid endoscope, a light source, and a high-speed camera, provides real-time information about pellet size and layer uniformity, enabling timely intervention in the case of out-of-spec products. A convolutional neural network-based instance segmentation algorithm was employed to detect particles in focus, ensuring that pellet size could be accurately determined despite the dense flow of the particles. After training the model, the performance of the developed system was assessed by analysing the particle size distribution of pellet cores with variable sizes within the 250–850 μm size range. The endoscopic system was tested in-line at a larger scale during the drug layering of inert pellet cores. The particle size data acquired in real time with the endoscopic imaging system corresponded with the reference methods, demonstrating the feasibility of the proposed machine vision-based method as a process analytical technology tool for in-line process monitoring.
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spelling doaj-art-ff9b46dc52eb45c2945d1f27cd4647fd2025-08-22T04:56:06ZengElsevierJournal of Pharmaceutical Analysis2095-17792025-08-0115810122710.1016/j.jpha.2025.101227Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering processOrsolya Péterfi0Nikolett Kállai-Szabó1Kincső Renáta Demeter2Ádám Tibor Barna3István Antal4Edina Szabó5Emese Sipos6Zsombor Kristóf Nagy7Dorián László Galata8Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111, Budapest, HungaryDepartment of Pharmaceutics, Semmelweis University, Hőgyes E. str 7, 1092, Budapest, Hungary; Center for Pharmacology and Drug Research & Development, Semmelweis University, Budapest, Hungary; Corresponding author. Department of Pharmaceutics, Semmelweis University, Hőgyes E. str 7, 1092, Budapest, Hungary.Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111, Budapest, HungaryDepartment of Pharmaceutics, Semmelweis University, Hőgyes E. str 7, 1092, Budapest, HungaryDepartment of Pharmaceutics, Semmelweis University, Hőgyes E. str 7, 1092, Budapest, Hungary; Center for Pharmacology and Drug Research & Development, Semmelweis University, Budapest, HungaryDepartment of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111, Budapest, HungaryDepartment of Pharmaceutical Industry and Management, Faculty of Pharmacy, George Emil Palade University of Medicine, Pharmacy, Sciences and Technology of Targu Mures, Gheorghe Marinescu Street 38, 540142, Targu Mures, RomaniaDepartment of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111, Budapest, Hungary; Corresponding author.Department of Organic Chemistry and Technology, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem rkp. 3., H-1111, Budapest, HungaryIn this study, an artificial intelligence-based machine vision system was developed for in-line particle size analysis during the pellet layering process. Drug-layered pellets were produced by coating microcrystalline cellulose cores with an ibuprofen-containing layering liquid until the target drug content was achieved. Drug content increases with pellet size; therefore, particle size monitoring can ensure product safety and quality. The direct imaging system, consisting of a rigid endoscope, a light source, and a high-speed camera, provides real-time information about pellet size and layer uniformity, enabling timely intervention in the case of out-of-spec products. A convolutional neural network-based instance segmentation algorithm was employed to detect particles in focus, ensuring that pellet size could be accurately determined despite the dense flow of the particles. After training the model, the performance of the developed system was assessed by analysing the particle size distribution of pellet cores with variable sizes within the 250–850 μm size range. The endoscopic system was tested in-line at a larger scale during the drug layering of inert pellet cores. The particle size data acquired in real time with the endoscopic imaging system corresponded with the reference methods, demonstrating the feasibility of the proposed machine vision-based method as a process analytical technology tool for in-line process monitoring.http://www.sciencedirect.com/science/article/pii/S2095177925000449Machine visionConvolutional neural networksIn-line monitoringEndoscopeParticle size distributionPellet layering
spellingShingle Orsolya Péterfi
Nikolett Kállai-Szabó
Kincső Renáta Demeter
Ádám Tibor Barna
István Antal
Edina Szabó
Emese Sipos
Zsombor Kristóf Nagy
Dorián László Galata
Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process
Journal of Pharmaceutical Analysis
Machine vision
Convolutional neural networks
In-line monitoring
Endoscope
Particle size distribution
Pellet layering
title Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process
title_full Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process
title_fullStr Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process
title_full_unstemmed Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process
title_short Artificial intelligence-aided endoscopic in-line particle size analysis during the pellet layering process
title_sort artificial intelligence aided endoscopic in line particle size analysis during the pellet layering process
topic Machine vision
Convolutional neural networks
In-line monitoring
Endoscope
Particle size distribution
Pellet layering
url http://www.sciencedirect.com/science/article/pii/S2095177925000449
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