VdAHA1 positively regulate pathogenicity in Verticillium dahliae
AHA1 (activator of HSP90 ATPase) is a co-chaperone protein that mainly performs its function by interacting with the HSP90. The biological function of AHA1 has been widely reported in many species. In this study, we knocked out the VdAHA1 gene of V. dahliae by homologous recombination method. The Vd...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1535187/full |
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| author | Dongpo Li Yuan Yuan Jinglong Zhou Jinglong Zhou Zili Feng Zili Feng Feng Wei Feng Wei Heqin Zhu Heqin Zhu Lihong Zhao Lihong Zhao Hongjie Feng Hongjie Feng Yalin Zhang Yalin Zhang |
| author_facet | Dongpo Li Yuan Yuan Jinglong Zhou Jinglong Zhou Zili Feng Zili Feng Feng Wei Feng Wei Heqin Zhu Heqin Zhu Lihong Zhao Lihong Zhao Hongjie Feng Hongjie Feng Yalin Zhang Yalin Zhang |
| author_sort | Dongpo Li |
| collection | DOAJ |
| description | AHA1 (activator of HSP90 ATPase) is a co-chaperone protein that mainly performs its function by interacting with the HSP90. The biological function of AHA1 has been widely reported in many species. In this study, we knocked out the VdAHA1 gene of V. dahliae by homologous recombination method. The VdAHA1 knockout mutants showed increased drug sensitivity to ergosterol synthesis pathway, significantly inhibiting ergosterol biosynthesis. The VdAHA1 knockout mutant strain also showed decreased melanin in microsclerotia by reduced expression of microsclerotia and melanin related genes Vaflm, Vayg1, and VdSCD. The VdAHA1 mutant showed decreased conidial production that were slightly damaged and showed more sensitivity to abiotic stresses such as temperature, SDS, CR, Sorbitol (SBT), NaCl, and KCl and decreased ATP contents. More importantly, the mutant was significantly less virulent to cotton than the wild type. This study identified the important functions of VdAHA1 in the growth, stress resistance, and virulence. |
| format | Article |
| id | doaj-art-ff980c1e4be046e18ecf96dbf1054849 |
| institution | OA Journals |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-ff980c1e4be046e18ecf96dbf10548492025-08-20T02:34:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-05-011610.3389/fmicb.2025.15351871535187VdAHA1 positively regulate pathogenicity in Verticillium dahliaeDongpo Li0Yuan Yuan1Jinglong Zhou2Jinglong Zhou3Zili Feng4Zili Feng5Feng Wei6Feng Wei7Heqin Zhu8Heqin Zhu9Lihong Zhao10Lihong Zhao11Hongjie Feng12Hongjie Feng13Yalin Zhang14Yalin Zhang15State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaState Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang, Henan, ChinaWestern Agricultural Research Center of Chinese Academy of Agricultural Sciences, Changji, Xinjiang, ChinaAHA1 (activator of HSP90 ATPase) is a co-chaperone protein that mainly performs its function by interacting with the HSP90. The biological function of AHA1 has been widely reported in many species. In this study, we knocked out the VdAHA1 gene of V. dahliae by homologous recombination method. The VdAHA1 knockout mutants showed increased drug sensitivity to ergosterol synthesis pathway, significantly inhibiting ergosterol biosynthesis. The VdAHA1 knockout mutant strain also showed decreased melanin in microsclerotia by reduced expression of microsclerotia and melanin related genes Vaflm, Vayg1, and VdSCD. The VdAHA1 mutant showed decreased conidial production that were slightly damaged and showed more sensitivity to abiotic stresses such as temperature, SDS, CR, Sorbitol (SBT), NaCl, and KCl and decreased ATP contents. More importantly, the mutant was significantly less virulent to cotton than the wild type. This study identified the important functions of VdAHA1 in the growth, stress resistance, and virulence.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1535187/fullAHA1Verticillium dahliaemicrosclerotiastress responsepathogenicity |
| spellingShingle | Dongpo Li Yuan Yuan Jinglong Zhou Jinglong Zhou Zili Feng Zili Feng Feng Wei Feng Wei Heqin Zhu Heqin Zhu Lihong Zhao Lihong Zhao Hongjie Feng Hongjie Feng Yalin Zhang Yalin Zhang VdAHA1 positively regulate pathogenicity in Verticillium dahliae Frontiers in Microbiology AHA1 Verticillium dahliae microsclerotia stress response pathogenicity |
| title | VdAHA1 positively regulate pathogenicity in Verticillium dahliae |
| title_full | VdAHA1 positively regulate pathogenicity in Verticillium dahliae |
| title_fullStr | VdAHA1 positively regulate pathogenicity in Verticillium dahliae |
| title_full_unstemmed | VdAHA1 positively regulate pathogenicity in Verticillium dahliae |
| title_short | VdAHA1 positively regulate pathogenicity in Verticillium dahliae |
| title_sort | vdaha1 positively regulate pathogenicity in verticillium dahliae |
| topic | AHA1 Verticillium dahliae microsclerotia stress response pathogenicity |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1535187/full |
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