Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey
Introduction: The purpose of this study was to investigate group B streptococcus (GBS) colonization, to compare the methods, to determine the relationship between GBS carriage and risk factors, and to genotype the GBS isolates. Methodology: Recto-vaginal swab specimens were obtained from 500 wome...
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The Journal of Infection in Developing Countries
2016-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/6190 |
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| author | Feyza Alp Duygu Findik Hatice Turk Dagi Ugur Arslan Aybike Tazegul Pekin Setenay Arzu Yilmaz |
| author_facet | Feyza Alp Duygu Findik Hatice Turk Dagi Ugur Arslan Aybike Tazegul Pekin Setenay Arzu Yilmaz |
| author_sort | Feyza Alp |
| collection | DOAJ |
| description |
Introduction: The purpose of this study was to investigate group B streptococcus (GBS) colonization, to compare the methods, to determine the relationship between GBS carriage and risk factors, and to genotype the GBS isolates.
Methodology: Recto-vaginal swab specimens were obtained from 500 women, and a questionnaire was administered to each to assess their risk factors for GBS carriage. A culture, GBS antigen test, and polymerase chain reaction (PCR) were performed on all samples. Antibiotic susceptibility testing was performed, and the clonal relationship was determined by pulsed-field gel electrophoresis (PFGE) on all viable isolates.
Results: Of the 500 women, sixty-eight (13.6%) women were GBS carriers, of whom 9.8% were pregnant and 16.5% not. There was a significant difference between GBS carriage and history of premature rupture of membrane (PROM). GBS was isolated from 65 (13%) samples. GBS was positive in 70 (14%) samples by antigen test and in 62 (12.4%) by PCR. Sixty-eight of the 70 positive antigen tests were confirmed by PCR or culture. Fifty-five isolates were resistant to tetracycline, 16 to erythromycin and clindamycin, and 13 to levofloxacin. Thirteen different pulsotypes and 17 sporadic strains were determined by PFGE.
Conclusions: GBS carriage rate in non-pregnant women was higher than in pregnant women. The GBS antigen test was more sensitive than culture and PCR. GBS isolates did not originate from a single clone and contained sporadic strains. There was a significant difference between GBS carriage and history of PROM. Epidemiologic data obtained in this study will help future studies. |
| format | Article |
| id | doaj-art-ff97489ebc194e68988ba24680a71b7b |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-ff97489ebc194e68988ba24680a71b7b2025-08-20T02:14:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-03-01100310.3855/jidc.6190Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in TurkeyFeyza Alp0Duygu Findik1Hatice Turk Dagi2Ugur Arslan3Aybike Tazegul Pekin4Setenay Arzu Yilmaz5Selcuk University, Faculty of Medicine, Konya, TurkeySelcuk University, Faculty of Medicine, Konya, TurkeySelcuk University, Faculty of Medicine, Konya, TurkeySelcuk University, Faculty of Medicine, Konya, TurkeySelcuk University, Faculty of Medicine, Konya, TurkeySelcuk University, Faculty of Medicine, Konya, Turkey Introduction: The purpose of this study was to investigate group B streptococcus (GBS) colonization, to compare the methods, to determine the relationship between GBS carriage and risk factors, and to genotype the GBS isolates. Methodology: Recto-vaginal swab specimens were obtained from 500 women, and a questionnaire was administered to each to assess their risk factors for GBS carriage. A culture, GBS antigen test, and polymerase chain reaction (PCR) were performed on all samples. Antibiotic susceptibility testing was performed, and the clonal relationship was determined by pulsed-field gel electrophoresis (PFGE) on all viable isolates. Results: Of the 500 women, sixty-eight (13.6%) women were GBS carriers, of whom 9.8% were pregnant and 16.5% not. There was a significant difference between GBS carriage and history of premature rupture of membrane (PROM). GBS was isolated from 65 (13%) samples. GBS was positive in 70 (14%) samples by antigen test and in 62 (12.4%) by PCR. Sixty-eight of the 70 positive antigen tests were confirmed by PCR or culture. Fifty-five isolates were resistant to tetracycline, 16 to erythromycin and clindamycin, and 13 to levofloxacin. Thirteen different pulsotypes and 17 sporadic strains were determined by PFGE. Conclusions: GBS carriage rate in non-pregnant women was higher than in pregnant women. The GBS antigen test was more sensitive than culture and PCR. GBS isolates did not originate from a single clone and contained sporadic strains. There was a significant difference between GBS carriage and history of PROM. Epidemiologic data obtained in this study will help future studies.https://jidc.org/index.php/journal/article/view/6190Group B streptococcuscarriageculturePCRGBS antigen testPFGE |
| spellingShingle | Feyza Alp Duygu Findik Hatice Turk Dagi Ugur Arslan Aybike Tazegul Pekin Setenay Arzu Yilmaz Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey Journal of Infection in Developing Countries Group B streptococcus carriage culture PCR GBS antigen test PFGE |
| title | Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey |
| title_full | Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey |
| title_fullStr | Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey |
| title_full_unstemmed | Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey |
| title_short | Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey |
| title_sort | screening and genotyping of group b streptococcus in pregnant and non pregnant women in turkey |
| topic | Group B streptococcus carriage culture PCR GBS antigen test PFGE |
| url | https://jidc.org/index.php/journal/article/view/6190 |
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