Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system
SARS-CoV-2 has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed a...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Emerging Microbes and Infections |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2021.1873073 |
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| author | Hsing-Yi Chung Ming-Jr Jian Chih-Kai Chang Jung-Chung Lin Kuo-Ming Yeh Chien-Wen Chen Sheng-Kang Chiu Yi-Hui Wang Shu-Jung Liao Shih-Yi Li Shan-Shan Hsieh Shih-Hung Tsai Cherng-Lih Perng Ji-Rong Yang Ming-Tsan Liu Feng-Yee Chang Hung-Sheng Shang |
| author_facet | Hsing-Yi Chung Ming-Jr Jian Chih-Kai Chang Jung-Chung Lin Kuo-Ming Yeh Chien-Wen Chen Sheng-Kang Chiu Yi-Hui Wang Shu-Jung Liao Shih-Yi Li Shan-Shan Hsieh Shih-Hung Tsai Cherng-Lih Perng Ji-Rong Yang Ming-Tsan Liu Feng-Yee Chang Hung-Sheng Shang |
| author_sort | Hsing-Yi Chung |
| collection | DOAJ |
| description | SARS-CoV-2 has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed and validated a rapid total nucleic acid extraction method based on real-time reverse transcription-polymerase chain reaction (RT-PCR) for the reliable, high-throughput simultaneous detection of common cold viral pathogens using the BD MAX Platform. The system was evaluated using 205 nasopharyngeal swab clinical samples. For assessment of the limit of detection (LoD), we used SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) RNA standards. The BD MAX dual multiplex real-time RT-PCR panel demonstrated a sensitivity comparable to that of the World Health Organization-recommended SARS-CoV-2 assay with an LoD of 50 copies/PCR. The LoD of influenza A/B and RSV was 100–200 copies/PCR. The overall percent agreement between the BD MAX panel and laboratory-developed RT-PCR test on 55 SARS-CoV-2-positive clinical samples was 100%. Among the 55 positive cases of COVID-19 analysed, no coinfection was detected. The BD MAX rapid multiplex PCR provides a highly sensitive, robust, and accurate assay for the rapid detection of SARS-CoV-2, influenza A/B, and RSV. |
| format | Article |
| id | doaj-art-ff95c37150bd479b96a94ea5d9a08a7d |
| institution | DOAJ |
| issn | 2222-1751 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj-art-ff95c37150bd479b96a94ea5d9a08a7d2025-08-20T02:59:11ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512021-01-0110116116610.1080/22221751.2021.1873073Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open systemHsing-Yi Chung0Ming-Jr Jian1Chih-Kai Chang2Jung-Chung Lin3Kuo-Ming Yeh4Chien-Wen Chen5Sheng-Kang Chiu6Yi-Hui Wang7Shu-Jung Liao8Shih-Yi Li9Shan-Shan Hsieh10Shih-Hung Tsai11Cherng-Lih Perng12Ji-Rong Yang13Ming-Tsan Liu14Feng-Yee Chang15Hung-Sheng Shang16Division of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Pulmonary and Critical Care Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDepartment of Emergency Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCCenters for Disease Control, Taipei, Taiwan, ROCCenters for Disease Control, Taipei, Taiwan, ROCDivision of Infectious Diseases and Tropical Medicine, Department of Medicine, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCDivision of Clinical Pathology, Department of Pathology, Tri-Service General Hospital, National Defense Medical Center, Taipei, Taiwan, ROCSARS-CoV-2 has spread rapidly, causing deaths worldwide. In this study, we evaluated the performance of the BD MAX Open System module for identifying viral pathogens, including SARS-CoV-2, in nasopharyngeal specimens from individuals with symptoms of upper respiratory tract infection. We developed and validated a rapid total nucleic acid extraction method based on real-time reverse transcription-polymerase chain reaction (RT-PCR) for the reliable, high-throughput simultaneous detection of common cold viral pathogens using the BD MAX Platform. The system was evaluated using 205 nasopharyngeal swab clinical samples. For assessment of the limit of detection (LoD), we used SARS-CoV-2, influenza A/B, and respiratory syncytial virus (RSV) RNA standards. The BD MAX dual multiplex real-time RT-PCR panel demonstrated a sensitivity comparable to that of the World Health Organization-recommended SARS-CoV-2 assay with an LoD of 50 copies/PCR. The LoD of influenza A/B and RSV was 100–200 copies/PCR. The overall percent agreement between the BD MAX panel and laboratory-developed RT-PCR test on 55 SARS-CoV-2-positive clinical samples was 100%. Among the 55 positive cases of COVID-19 analysed, no coinfection was detected. The BD MAX rapid multiplex PCR provides a highly sensitive, robust, and accurate assay for the rapid detection of SARS-CoV-2, influenza A/B, and RSV.https://www.tandfonline.com/doi/10.1080/22221751.2021.1873073SARS-CoV-2COVID-19influenzarespiratory syncytial virussimultaneous detectionreal-time PCR |
| spellingShingle | Hsing-Yi Chung Ming-Jr Jian Chih-Kai Chang Jung-Chung Lin Kuo-Ming Yeh Chien-Wen Chen Sheng-Kang Chiu Yi-Hui Wang Shu-Jung Liao Shih-Yi Li Shan-Shan Hsieh Shih-Hung Tsai Cherng-Lih Perng Ji-Rong Yang Ming-Tsan Liu Feng-Yee Chang Hung-Sheng Shang Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system Emerging Microbes and Infections SARS-CoV-2 COVID-19 influenza respiratory syncytial virus simultaneous detection real-time PCR |
| title | Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system |
| title_full | Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system |
| title_fullStr | Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system |
| title_full_unstemmed | Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system |
| title_short | Novel dual multiplex real-time RT-PCR assays for the rapid detection of SARS-CoV-2, influenza A/B, and respiratory syncytial virus using the BD MAX open system |
| title_sort | novel dual multiplex real time rt pcr assays for the rapid detection of sars cov 2 influenza a b and respiratory syncytial virus using the bd max open system |
| topic | SARS-CoV-2 COVID-19 influenza respiratory syncytial virus simultaneous detection real-time PCR |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2021.1873073 |
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