HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones

Radiotherapy (RT) is a highly valuable method in cancer therapy, but its therapeutic efficacy is limited by its side effects and tumour radiation resistance. The resistance is mainly induced by hypoxia in the tumour microenvironment (TME). As a nano-oxygen carrier, Haemoglobin-based oxygen carriers...

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Main Authors: Yingcan Xu, Kehui Zhu, Jiakang Wu, Shifan Zheng, Rui Zhong, Wentao Zhou, Ye Cao, Jiaxin Liu, Hong Wang
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Artificial Cells, Nanomedicine, and Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/21691401.2023.2276768
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author Yingcan Xu
Kehui Zhu
Jiakang Wu
Shifan Zheng
Rui Zhong
Wentao Zhou
Ye Cao
Jiaxin Liu
Hong Wang
author_facet Yingcan Xu
Kehui Zhu
Jiakang Wu
Shifan Zheng
Rui Zhong
Wentao Zhou
Ye Cao
Jiaxin Liu
Hong Wang
author_sort Yingcan Xu
collection DOAJ
description Radiotherapy (RT) is a highly valuable method in cancer therapy, but its therapeutic efficacy is limited by its side effects and tumour radiation resistance. The resistance is mainly induced by hypoxia in the tumour microenvironment (TME). As a nano-oxygen carrier, Haemoglobin-based oxygen carriers (HBOCs) administration is a promising strategy to alleviate tumour hypoxia which may remodel TME to ameliorate radiation resistance and enable RT more effective. In this study, we administered fractionated RT combined with HBOC to treat Miapaca-2 cell and Hela cell xenografts on nude mice. The study found that HBOC relieved hypoxic environment and down-regulate expression of hypoxia-inducible factor-1α (Hif-1α) both in regular (100 mm3) and large (360/400 mm3) tumours. The proliferation and metastasis of tumour tissue also decreased after HBOC application. Nevertheless, in vivo RT combined with HBOC performed more effectively to suppress tumour growth in large tumours than in regular tumours. This is due to more severe hypoxic regions exist in the large solid tumours compared to the regular counterparts, and HBOC administration may be more effective in alleviating hypoxia in large tumours. Thus, HBOC sensitization therapy is more suitable for large solid tumours.
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publisher Taylor & Francis Group
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series Artificial Cells, Nanomedicine, and Biotechnology
spelling doaj-art-ff8e96efc08242aeb1a8f3767a5be1ee2025-08-20T02:19:16ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2024-12-0152111410.1080/21691401.2023.2276768HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular onesYingcan Xu0Kehui Zhu1Jiakang Wu2Shifan Zheng3Rui Zhong4Wentao Zhou5Ye Cao6Jiaxin Liu7Hong Wang8Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences, Peking Union Medical College, Chengdu, ChinaRadiotherapy (RT) is a highly valuable method in cancer therapy, but its therapeutic efficacy is limited by its side effects and tumour radiation resistance. The resistance is mainly induced by hypoxia in the tumour microenvironment (TME). As a nano-oxygen carrier, Haemoglobin-based oxygen carriers (HBOCs) administration is a promising strategy to alleviate tumour hypoxia which may remodel TME to ameliorate radiation resistance and enable RT more effective. In this study, we administered fractionated RT combined with HBOC to treat Miapaca-2 cell and Hela cell xenografts on nude mice. The study found that HBOC relieved hypoxic environment and down-regulate expression of hypoxia-inducible factor-1α (Hif-1α) both in regular (100 mm3) and large (360/400 mm3) tumours. The proliferation and metastasis of tumour tissue also decreased after HBOC application. Nevertheless, in vivo RT combined with HBOC performed more effectively to suppress tumour growth in large tumours than in regular tumours. This is due to more severe hypoxic regions exist in the large solid tumours compared to the regular counterparts, and HBOC administration may be more effective in alleviating hypoxia in large tumours. Thus, HBOC sensitization therapy is more suitable for large solid tumours.https://www.tandfonline.com/doi/10.1080/21691401.2023.2276768Haemoglobin-based oxygen carriers (HBOCs)tumor hypoxiaradiotherapyxenograftHif-1α
spellingShingle Yingcan Xu
Kehui Zhu
Jiakang Wu
Shifan Zheng
Rui Zhong
Wentao Zhou
Ye Cao
Jiaxin Liu
Hong Wang
HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
Artificial Cells, Nanomedicine, and Biotechnology
Haemoglobin-based oxygen carriers (HBOCs)
tumor hypoxia
radiotherapy
xenograft
Hif-1α
title HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
title_full HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
title_fullStr HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
title_full_unstemmed HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
title_short HBOC alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
title_sort hboc alleviated tumour hypoxia during radiotherapy more intensely in large solid tumours than regular ones
topic Haemoglobin-based oxygen carriers (HBOCs)
tumor hypoxia
radiotherapy
xenograft
Hif-1α
url https://www.tandfonline.com/doi/10.1080/21691401.2023.2276768
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