Fecal galectin-1 as a potential marker for colorectal cancer and disease severity
Background/Aim. Colorectal cancer (CRC) represents one of the most common cancers worldwide. CRC is frequently diagnosed at advanced stages with poor prognosis, indicating the need for new diagnostic and prognostic markers. The aim of this study was to determine systemic and fecal values of galectin...
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Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade
2019-01-01
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| Series: | Vojnosanitetski Pregled |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501800007J.pdf |
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| author | Jovanović Milan Gajović Nevena Zdravković Nataša Jovanović Marina Jurišević Milena Vojvodić Danilo Mirković Darko Milev Boško Marić Veljko Arsenijević Nebojša |
| author_facet | Jovanović Milan Gajović Nevena Zdravković Nataša Jovanović Marina Jurišević Milena Vojvodić Danilo Mirković Darko Milev Boško Marić Veljko Arsenijević Nebojša |
| author_sort | Jovanović Milan |
| collection | DOAJ |
| description | Background/Aim. Colorectal cancer (CRC) represents one of the most common cancers worldwide. CRC is frequently diagnosed at advanced stages with poor prognosis, indicating the need for new diagnostic and prognostic markers. The aim of this study was to determine systemic and fecal values of galectin- 1 (gal-1) and ratios between gal-1 and proinflammatory cytokines: tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interferon gamma (IFN-γ), in the patients with CRC and the relationship with clinicopathological aspects of the disease. Methods. The blood samples and feces liquid fraction of 58 patients with CRC were analyzed. The serum and fecal levels of TNF-α, IL-1β and IFN-γ and gal-1 were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Results. The fecal level of gal-1 was increased in the CRC patients with higher nuclear grade and poor tumor tissue differentiation. The gal-1/TNF-α ratio in the serum and feces had a higher trend in the patients with the advanced tumor-nodemetastasis (TNM) stage as well as the detectable lymphatic and blood vessel invasion. The gal-1/TNF-α and gal-1/IFN-γ ratios were increased in the serum of patients with presence of lung/liver metastasis or peritoneal carcinomatosis, while the enhanced gal-1/IL-1 ratio was detected only in the serum of patients with lung metastasis. A positive correlation between the gal-1 value in feces and histological differentiation of tumor and biomarkers alpha-fetoprotein (AFP) and cancer antigen- 19-9 (CA 19-9), respectively, was also observed. The fecal values of gal-1 higher than 13,708.29 pg/g presented a highly sensitive and specific marker for histological differentiation of tumor tissue. Conclusion. We believe that the predomination of gal-1 over pro-inflammatory cytokines TNF-α, IL-1β and IFN- γ in the patients with advanced and progressive CRC may implicate on an immunomodulatory role of gal-1 in the limiting ongoing proinflammatory processes. The fecal values of gal-1 can be used as a valuable marker for the severity of CRC. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175071, Grant no. 175069 and Grant no. 175103] |
| format | Article |
| id | doaj-art-ff8dfe7e1fa24f5fbd2edca801d1cc59 |
| institution | OA Journals |
| issn | 0042-8450 2406-0720 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Ministry of Defence of the Republic of Serbia, University of Defence, Belgrade |
| record_format | Article |
| series | Vojnosanitetski Pregled |
| spelling | doaj-art-ff8dfe7e1fa24f5fbd2edca801d1cc592025-08-20T02:04:48ZengMinistry of Defence of the Republic of Serbia, University of Defence, BelgradeVojnosanitetski Pregled0042-84502406-07202019-01-0176101037104410.2298/VSP171201007J0042-84501800007JFecal galectin-1 as a potential marker for colorectal cancer and disease severityJovanović Milan0Gajović Nevena1Zdravković Nataša2Jovanović Marina3Jurišević Milena4Vojvodić Danilo5Mirković Darko6Milev Boško7Marić Veljko8Arsenijević Nebojša9Military Medical Academy, Clinic for General Surgery, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, Serbia University of Kragujevac, Faculty of Medical Sciences, Department of Internal Medicine, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Internal Medicine, Kragujevac, SerbiaUniversity of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, SerbiaUniversity of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia + Military Medical Academy, Institute for Medical Research, Belgrade, SerbiaMilitary Medical Academy, Clinic for General Surgery, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaMilitary Medical Academy, Clinic for General Surgery, Belgrade, Serbia + University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, SerbiaUniversity of East Sarajevo, Faculty of Medicine, Department of Surgery, Foča, Bosnia and Herzegovina University of Kragujevac, Faculty of Medical Sciences, Center for Molecular Medicine and Stem Cell Research, Kragujevac, Serbia Background/Aim. Colorectal cancer (CRC) represents one of the most common cancers worldwide. CRC is frequently diagnosed at advanced stages with poor prognosis, indicating the need for new diagnostic and prognostic markers. The aim of this study was to determine systemic and fecal values of galectin- 1 (gal-1) and ratios between gal-1 and proinflammatory cytokines: tumor necrosis factor-alpha (TNF-α), interleukin-1 beta (IL-1β) and interferon gamma (IFN-γ), in the patients with CRC and the relationship with clinicopathological aspects of the disease. Methods. The blood samples and feces liquid fraction of 58 patients with CRC were analyzed. The serum and fecal levels of TNF-α, IL-1β and IFN-γ and gal-1 were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Results. The fecal level of gal-1 was increased in the CRC patients with higher nuclear grade and poor tumor tissue differentiation. The gal-1/TNF-α ratio in the serum and feces had a higher trend in the patients with the advanced tumor-nodemetastasis (TNM) stage as well as the detectable lymphatic and blood vessel invasion. The gal-1/TNF-α and gal-1/IFN-γ ratios were increased in the serum of patients with presence of lung/liver metastasis or peritoneal carcinomatosis, while the enhanced gal-1/IL-1 ratio was detected only in the serum of patients with lung metastasis. A positive correlation between the gal-1 value in feces and histological differentiation of tumor and biomarkers alpha-fetoprotein (AFP) and cancer antigen- 19-9 (CA 19-9), respectively, was also observed. The fecal values of gal-1 higher than 13,708.29 pg/g presented a highly sensitive and specific marker for histological differentiation of tumor tissue. Conclusion. We believe that the predomination of gal-1 over pro-inflammatory cytokines TNF-α, IL-1β and IFN- γ in the patients with advanced and progressive CRC may implicate on an immunomodulatory role of gal-1 in the limiting ongoing proinflammatory processes. The fecal values of gal-1 can be used as a valuable marker for the severity of CRC. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. 175071, Grant no. 175069 and Grant no. 175103]http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501800007J.pdfcolorectal neoplasmscarcinomafecesgalectin-1disease progression |
| spellingShingle | Jovanović Milan Gajović Nevena Zdravković Nataša Jovanović Marina Jurišević Milena Vojvodić Danilo Mirković Darko Milev Boško Marić Veljko Arsenijević Nebojša Fecal galectin-1 as a potential marker for colorectal cancer and disease severity Vojnosanitetski Pregled colorectal neoplasms carcinoma feces galectin-1 disease progression |
| title | Fecal galectin-1 as a potential marker for colorectal cancer and disease severity |
| title_full | Fecal galectin-1 as a potential marker for colorectal cancer and disease severity |
| title_fullStr | Fecal galectin-1 as a potential marker for colorectal cancer and disease severity |
| title_full_unstemmed | Fecal galectin-1 as a potential marker for colorectal cancer and disease severity |
| title_short | Fecal galectin-1 as a potential marker for colorectal cancer and disease severity |
| title_sort | fecal galectin 1 as a potential marker for colorectal cancer and disease severity |
| topic | colorectal neoplasms carcinoma feces galectin-1 disease progression |
| url | http://www.doiserbia.nb.rs/img/doi/0042-8450/2019/0042-84501800007J.pdf |
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