Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.)
Chilling stress inhibits the growth of okra (<i>Abelmoschus esculentus</i> L.), reduces its overall agricultural yield, and deteriorates fruit quality. Therefore, it is crucial to elucidate the mechanism through which okra plants respond to chilling stress. This study investigates the mo...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-04-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/14/7/1100 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849769302730735616 |
|---|---|
| author | Weixia Liu Jielin Wang Dan Zhu Xiaomin Yin Gongfu Du Yuling Qin Zhiyuan Zhang Ziji Liu |
| author_facet | Weixia Liu Jielin Wang Dan Zhu Xiaomin Yin Gongfu Du Yuling Qin Zhiyuan Zhang Ziji Liu |
| author_sort | Weixia Liu |
| collection | DOAJ |
| description | Chilling stress inhibits the growth of okra (<i>Abelmoschus esculentus</i> L.), reduces its overall agricultural yield, and deteriorates fruit quality. Therefore, it is crucial to elucidate the mechanism through which okra plants respond to chilling stress. This study investigates the molecular mechanisms of chilling tolerance by comparing the transcriptome and metabolome of chilling-tolerant (Ae182) and chilling-sensitive (Ae171) okra varieties. We found that Ae182 exhibits higher antioxidant enzyme activities, including SOD, POD, CAT, and APX, suggesting it mitigates oxidative stress more effectively than Ae171. Metabolomics analysis revealed that Ae182 produces higher levels of jasmonic acid (JA) and JA-isoleucine (JA-Ile) under chilling stress, potentially activating genes that alleviate oxidative damage. Additionally, integrated analyses identified key transcription factors, such as AP2, BHLH, and MYB, associated with JA and chilling stress. These findings provide candidate genes for further research on chilling resistance in okra. |
| format | Article |
| id | doaj-art-ff87b06ae3f04ba5945e42e99d76977b |
| institution | DOAJ |
| issn | 2223-7747 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj-art-ff87b06ae3f04ba5945e42e99d76977b2025-08-20T03:03:27ZengMDPI AGPlants2223-77472025-04-01147110010.3390/plants14071100Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.)Weixia Liu0Jielin Wang1Dan Zhu2Xiaomin Yin3Gongfu Du4Yuling Qin5Zhiyuan Zhang6Ziji Liu7Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, ChinaHainan Institute of Zhejiang University, Sanya 572025, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, ChinaHainan Institute of Zhejiang University, Sanya 572025, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture and Rual Affairs/Key Laboratory of Tropical Crops Germplasm Resources Genetic Improvement and Innovation of Hainan Province, Haikou 571101, ChinaChilling stress inhibits the growth of okra (<i>Abelmoschus esculentus</i> L.), reduces its overall agricultural yield, and deteriorates fruit quality. Therefore, it is crucial to elucidate the mechanism through which okra plants respond to chilling stress. This study investigates the molecular mechanisms of chilling tolerance by comparing the transcriptome and metabolome of chilling-tolerant (Ae182) and chilling-sensitive (Ae171) okra varieties. We found that Ae182 exhibits higher antioxidant enzyme activities, including SOD, POD, CAT, and APX, suggesting it mitigates oxidative stress more effectively than Ae171. Metabolomics analysis revealed that Ae182 produces higher levels of jasmonic acid (JA) and JA-isoleucine (JA-Ile) under chilling stress, potentially activating genes that alleviate oxidative damage. Additionally, integrated analyses identified key transcription factors, such as AP2, BHLH, and MYB, associated with JA and chilling stress. These findings provide candidate genes for further research on chilling resistance in okra.https://www.mdpi.com/2223-7747/14/7/1100<i>Abelmoschus esculentus</i> L.chilling stressJAtranscriptomemetabolome |
| spellingShingle | Weixia Liu Jielin Wang Dan Zhu Xiaomin Yin Gongfu Du Yuling Qin Zhiyuan Zhang Ziji Liu Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) Plants <i>Abelmoschus esculentus</i> L. chilling stress JA transcriptome metabolome |
| title | Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_full | Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_fullStr | Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_full_unstemmed | Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_short | Jasmonic Acid-Mediated Antioxidant Defense Confers Chilling Tolerance in Okra (<i>Abelmoschus esculentus</i> L.) |
| title_sort | jasmonic acid mediated antioxidant defense confers chilling tolerance in okra i abelmoschus esculentus i l |
| topic | <i>Abelmoschus esculentus</i> L. chilling stress JA transcriptome metabolome |
| url | https://www.mdpi.com/2223-7747/14/7/1100 |
| work_keys_str_mv | AT weixialiu jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT jielinwang jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT danzhu jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT xiaominyin jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT gongfudu jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT yulingqin jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT zhiyuanzhang jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil AT zijiliu jasmonicacidmediatedantioxidantdefenseconferschillingtoleranceinokraiabelmoschusesculentusil |