Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus
Background. Type 2 diabetes mellitus (T2DM) is determined by genetic and environmental factors. There have been many studies on the relationship between the composition of the gastrointestinal bacterial flora, T2DM, and obesity. There are no data, however, on the gut microbiome structure in monogeni...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/3876764 |
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| author | Sandra Mrozinska Piotr Radkowski Tomasz Gosiewski Magdalena Szopa Malgorzata Bulanda Agnieszka H. Ludwig-Galezowska Iwona Morawska Agnieszka Sroka-Oleksiak Bartlomiej Matejko Przemyslaw Kapusta Dominika Salamon Maciej T. Malecki Pawel Wolkow Tomasz Klupa |
| author_facet | Sandra Mrozinska Piotr Radkowski Tomasz Gosiewski Magdalena Szopa Malgorzata Bulanda Agnieszka H. Ludwig-Galezowska Iwona Morawska Agnieszka Sroka-Oleksiak Bartlomiej Matejko Przemyslaw Kapusta Dominika Salamon Maciej T. Malecki Pawel Wolkow Tomasz Klupa |
| author_sort | Sandra Mrozinska |
| collection | DOAJ |
| description | Background. Type 2 diabetes mellitus (T2DM) is determined by genetic and environmental factors. There have been many studies on the relationship between the composition of the gastrointestinal bacterial flora, T2DM, and obesity. There are no data, however, on the gut microbiome structure in monogenic forms of the disease including Maturity Onset Diabetes of the Young (MODY). Methods. The aim of the investigation was to compare the qualitative parameters of the colonic flora in patients with HNF1A-MODY and T2DM and healthy individuals. 16S sequencing of bacterial DNA isolated from the collected fecal samples using the MiSeq platform was performed. Results. There were significant between-group differences in the bacterial profile. At the phylum level, the amount of Proteobacteria was higher (p=0.0006) and the amount of Bacteroidetes was lower (p=0.0005) in T2DM group in comparison to the control group. In HNF1A-MODY group, the frequency of Bacteroidetes was lower than in the control group (p=0.0143). At the order level, Turicibacterales was more abundant in HNF1A-MODY group than in T2DM group. Conclusions. It appears that there are differences in the gut microbiome composition between patients with HNF1A-MODY and type 2 diabetes. Further investigation on this matter should be conducted. |
| format | Article |
| id | doaj-art-ff7d8f036d6745b59e569cfea4143f4d |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-ff7d8f036d6745b59e569cfea4143f4d2025-02-03T05:51:58ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/38767643876764Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes MellitusSandra Mrozinska0Piotr Radkowski1Tomasz Gosiewski2Magdalena Szopa3Malgorzata Bulanda4Agnieszka H. Ludwig-Galezowska5Iwona Morawska6Agnieszka Sroka-Oleksiak7Bartlomiej Matejko8Przemyslaw Kapusta9Dominika Salamon10Maciej T. Malecki11Pawel Wolkow12Tomasz Klupa13Department of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandCenter for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, PolandDepartment of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandDepartment of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandDepartment of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandCenter for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, PolandDepartment of Microbiology, Jagiellonian University Medical College, 18 Czysta Street, 31-121 Kraków, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandCenter for Medical Genomics OMICRON, Jagiellonian University Medical College, 7c Kopernika Street, 31-034 Kraków, PolandDepartment of Metabolic Diseases, Jagiellonian University Medical College, 15 Kopernika Street, 31-501 Kraków, PolandBackground. Type 2 diabetes mellitus (T2DM) is determined by genetic and environmental factors. There have been many studies on the relationship between the composition of the gastrointestinal bacterial flora, T2DM, and obesity. There are no data, however, on the gut microbiome structure in monogenic forms of the disease including Maturity Onset Diabetes of the Young (MODY). Methods. The aim of the investigation was to compare the qualitative parameters of the colonic flora in patients with HNF1A-MODY and T2DM and healthy individuals. 16S sequencing of bacterial DNA isolated from the collected fecal samples using the MiSeq platform was performed. Results. There were significant between-group differences in the bacterial profile. At the phylum level, the amount of Proteobacteria was higher (p=0.0006) and the amount of Bacteroidetes was lower (p=0.0005) in T2DM group in comparison to the control group. In HNF1A-MODY group, the frequency of Bacteroidetes was lower than in the control group (p=0.0143). At the order level, Turicibacterales was more abundant in HNF1A-MODY group than in T2DM group. Conclusions. It appears that there are differences in the gut microbiome composition between patients with HNF1A-MODY and type 2 diabetes. Further investigation on this matter should be conducted.http://dx.doi.org/10.1155/2016/3876764 |
| spellingShingle | Sandra Mrozinska Piotr Radkowski Tomasz Gosiewski Magdalena Szopa Malgorzata Bulanda Agnieszka H. Ludwig-Galezowska Iwona Morawska Agnieszka Sroka-Oleksiak Bartlomiej Matejko Przemyslaw Kapusta Dominika Salamon Maciej T. Malecki Pawel Wolkow Tomasz Klupa Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus Journal of Diabetes Research |
| title | Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus |
| title_full | Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus |
| title_fullStr | Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus |
| title_full_unstemmed | Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus |
| title_short | Qualitative Parameters of the Colonic Flora in Patients with HNF1A-MODY Are Different from Those Observed in Type 2 Diabetes Mellitus |
| title_sort | qualitative parameters of the colonic flora in patients with hnf1a mody are different from those observed in type 2 diabetes mellitus |
| url | http://dx.doi.org/10.1155/2016/3876764 |
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