Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines
Superparamagnetic iron oxide nanoparticles (SPION) have demonstrated potential biomedical application, mainly for cancer treatment. In this work, SPION were successfully synthesized by the coprecipitation method, followed by coating and functionalization with 3-aminopropyl- triethoxysilane (APTES)....
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| Format: | Article |
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IOP Publishing
2025-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/add098 |
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| author | Viviana Sandoval-Flores J Angelica Ortega-Cardenas Eva Ramon-Gallegos Gustavo F Gutiérrez-López David Ravelo-Acuña Hernani Yee-Madeira Rosalva Mora-Escobedo |
| author_facet | Viviana Sandoval-Flores J Angelica Ortega-Cardenas Eva Ramon-Gallegos Gustavo F Gutiérrez-López David Ravelo-Acuña Hernani Yee-Madeira Rosalva Mora-Escobedo |
| author_sort | Viviana Sandoval-Flores |
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| description | Superparamagnetic iron oxide nanoparticles (SPION) have demonstrated potential biomedical application, mainly for cancer treatment. In this work, SPION were successfully synthesized by the coprecipitation method, followed by coating and functionalization with 3-aminopropyl- triethoxysilane (APTES). The synthesized samples (SPION and SPION@APTES) were characterized by their microscopic morphology, electrical charge, composition, and their superparamagnetic behavior was confirmed by SQUID analysis. The aqueous stability of SPION and SPION@APTES was significantly improved upon APTES coating, enhancing their potential as therapeutic molecules. Evaluation of cell viability upon exposure to SPION was assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in three cell- lines (non-tumoral HaCaT, tumoral cervical HeLa, and MDA-MB-231 breast cancer). HeLa cells showed greater tolerance to both samples of SPION than the other cell lines. Conversely, MDA- MB-231 and HaCaT cells showed significant differences between SPION and SPION@APTES. A dose-dependent decrease in cell viability was observed in the three cell-lines at concentrations >200 μg ml ^−1 for both samples SPION and SPION@APTES. It was demonstrated that dosage, exposure time, and the use of specific cell-lines must be considered when assessing SPION toxicity and its therapeutic potential. |
| format | Article |
| id | doaj-art-ff7c7218a0c14a06ae84ff7872d92141 |
| institution | Kabale University |
| issn | 2053-1591 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj-art-ff7c7218a0c14a06ae84ff7872d921412025-08-20T03:52:11ZengIOP PublishingMaterials Research Express2053-15912025-01-0112505540110.1088/2053-1591/add098Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-linesViviana Sandoval-Flores0https://orcid.org/0009-0001-8558-3713J Angelica Ortega-Cardenas1https://orcid.org/0000-0001-6678-4109Eva Ramon-Gallegos2Gustavo F Gutiérrez-López3https://orcid.org/0000-0002-8171-5977David Ravelo-Acuña4https://orcid.org/0000-0002-6315-7721Hernani Yee-Madeira5Rosalva Mora-Escobedo6https://orcid.org/0000-0003-3197-1187Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Departamento de Ingeniería Bioquímica, Ciudad de México, CDMX, CP. 07738, MexicoInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Departamento de Ingeniería Bioquímica, Ciudad de México, CDMX, CP. 07738, MexicoInstituto Politécnico Nacional Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Laboratorio de Citopatología Ambiental, Departamento de Morfología, Ciudad de México, CDMX CP. 07738, MexicoInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Departamento de Ingeniería Bioquímica, Ciudad de México, CDMX, CP. 07738, MexicoCentro de Investigación y Estudios Avanzados, Zacatenco, CDMX, CP. 07360, MexicoInstituto Politécnico Nacional Escuela Superior de Física y Matemáticas, Laboratorio de Espectroscopia Mössbauer y Técnicas Complementarias, Unidad Profesional Adolfo López Mateos, Ciudad de México, CDMX, CP. 07738, MexicoInstituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Campus Zacatenco, Departamento de Ingeniería Bioquímica, Ciudad de México, CDMX, CP. 07738, MexicoSuperparamagnetic iron oxide nanoparticles (SPION) have demonstrated potential biomedical application, mainly for cancer treatment. In this work, SPION were successfully synthesized by the coprecipitation method, followed by coating and functionalization with 3-aminopropyl- triethoxysilane (APTES). The synthesized samples (SPION and SPION@APTES) were characterized by their microscopic morphology, electrical charge, composition, and their superparamagnetic behavior was confirmed by SQUID analysis. The aqueous stability of SPION and SPION@APTES was significantly improved upon APTES coating, enhancing their potential as therapeutic molecules. Evaluation of cell viability upon exposure to SPION was assessed through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in three cell- lines (non-tumoral HaCaT, tumoral cervical HeLa, and MDA-MB-231 breast cancer). HeLa cells showed greater tolerance to both samples of SPION than the other cell lines. Conversely, MDA- MB-231 and HaCaT cells showed significant differences between SPION and SPION@APTES. A dose-dependent decrease in cell viability was observed in the three cell-lines at concentrations >200 μg ml ^−1 for both samples SPION and SPION@APTES. It was demonstrated that dosage, exposure time, and the use of specific cell-lines must be considered when assessing SPION toxicity and its therapeutic potential.https://doi.org/10.1088/2053-1591/add098antioxidant activitycancersuperparamagnetic iron oxide nanoparticles |
| spellingShingle | Viviana Sandoval-Flores J Angelica Ortega-Cardenas Eva Ramon-Gallegos Gustavo F Gutiérrez-López David Ravelo-Acuña Hernani Yee-Madeira Rosalva Mora-Escobedo Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines Materials Research Express antioxidant activity cancer superparamagnetic iron oxide nanoparticles |
| title | Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines |
| title_full | Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines |
| title_fullStr | Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines |
| title_full_unstemmed | Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines |
| title_short | Characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non-cancerous cell-lines |
| title_sort | characterization of superparamagnetic iron oxide nanoparticles and their effect on viability in cancer and non cancerous cell lines |
| topic | antioxidant activity cancer superparamagnetic iron oxide nanoparticles |
| url | https://doi.org/10.1088/2053-1591/add098 |
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