Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.

Bacteremia, the presence of viable bacteria in the blood stream, is often associated with several clinical conditions. Bacteremia can lead to multiple organ failure if managed incorrectly, which makes providing suitable nutritional support vital for reducing bacteremia-associated mortality. In order...

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Main Authors: Fangcong Dong, Bin Wang, Lulu Zhang, Huiru Tang, Jieshou Li, Yulan Wang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051060&type=printable
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author Fangcong Dong
Bin Wang
Lulu Zhang
Huiru Tang
Jieshou Li
Yulan Wang
author_facet Fangcong Dong
Bin Wang
Lulu Zhang
Huiru Tang
Jieshou Li
Yulan Wang
author_sort Fangcong Dong
collection DOAJ
description Bacteremia, the presence of viable bacteria in the blood stream, is often associated with several clinical conditions. Bacteremia can lead to multiple organ failure if managed incorrectly, which makes providing suitable nutritional support vital for reducing bacteremia-associated mortality. In order to provide such information, we investigated the metabolic consequences of a Klebsiella pneumoniae (K. pneumoniae) infection in vivo by employing a combination of (1)H nuclear magnetic resonance spectroscopy and multivariate data analysis. K. pneumoniae was intravenously infused in rats; urine and plasma samples were collected at different time intervals. We found that K. pneumoniae-induced bacteremia stimulated glycolysis and the tricarboxylic acid cycle and also promoted oxidation of fatty acids and creatine phosphate to facilitate the energy-demanding host response. In addition, K. pneumoniae bacteremia also induced anti-endotoxin, anti-inflammatory and anti-oxidization responses in the host. Furthermore, bacteremia could cause a disturbance in the gut microbiotal functions as suggested by alterations in a range of amines and bacteria-host co-metabolites. Our results suggest that supplementation with glucose and a high-fat and choline-rich diet could ameliorate the burdens associated with bacteremia. Our research provides underlying pathological processes of bacteremia and a better understanding of the clinical and biochemical manifestations of bacteremia.
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spelling doaj-art-ff7a331dfdf548aa838dbad4da1b19d12025-08-20T02:19:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01711e5106010.1371/journal.pone.0051060Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.Fangcong DongBin WangLulu ZhangHuiru TangJieshou LiYulan WangBacteremia, the presence of viable bacteria in the blood stream, is often associated with several clinical conditions. Bacteremia can lead to multiple organ failure if managed incorrectly, which makes providing suitable nutritional support vital for reducing bacteremia-associated mortality. In order to provide such information, we investigated the metabolic consequences of a Klebsiella pneumoniae (K. pneumoniae) infection in vivo by employing a combination of (1)H nuclear magnetic resonance spectroscopy and multivariate data analysis. K. pneumoniae was intravenously infused in rats; urine and plasma samples were collected at different time intervals. We found that K. pneumoniae-induced bacteremia stimulated glycolysis and the tricarboxylic acid cycle and also promoted oxidation of fatty acids and creatine phosphate to facilitate the energy-demanding host response. In addition, K. pneumoniae bacteremia also induced anti-endotoxin, anti-inflammatory and anti-oxidization responses in the host. Furthermore, bacteremia could cause a disturbance in the gut microbiotal functions as suggested by alterations in a range of amines and bacteria-host co-metabolites. Our results suggest that supplementation with glucose and a high-fat and choline-rich diet could ameliorate the burdens associated with bacteremia. Our research provides underlying pathological processes of bacteremia and a better understanding of the clinical and biochemical manifestations of bacteremia.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051060&type=printable
spellingShingle Fangcong Dong
Bin Wang
Lulu Zhang
Huiru Tang
Jieshou Li
Yulan Wang
Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
PLoS ONE
title Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
title_full Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
title_fullStr Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
title_full_unstemmed Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
title_short Metabolic response to Klebsiella pneumoniae infection in an experimental rat model.
title_sort metabolic response to klebsiella pneumoniae infection in an experimental rat model
url https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0051060&type=printable
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AT huirutang metabolicresponsetoklebsiellapneumoniaeinfectioninanexperimentalratmodel
AT jieshouli metabolicresponsetoklebsiellapneumoniaeinfectioninanexperimentalratmodel
AT yulanwang metabolicresponsetoklebsiellapneumoniaeinfectioninanexperimentalratmodel