Research advances on pathogenic nucleic acid detection technology based on CRIPSR/Cas system
Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) system, an ancient bacterial and archaeal immune system, has rapidly developed into a popular gene-editing tool, which largely promotes the development of several biology-related fields. B...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2023-10-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2022.08.051 |
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| Summary: | Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) (CRISPR/Cas) system, an ancient bacterial and archaeal immune system, has rapidly developed into a popular gene-editing tool, which largely promotes the development of several biology-related fields. By combining the CRISPR/Cas systems with the isothermal amplification techniques, the novel and effective detection methods with high sensitivity and independence of equipment have been established, such as DNA endonuclease-targeted CRISPR trans-reporter (DETECTR) and specific high-sensitivity enzymatic reporter unlocking (SHERLOCK). These new technologies not only improve the performance of the CRISPR/Cas system in different situations, but also inspire its application potential in the on-site detection. In this review, we summarized the nucleic acid detection methods developed on the three widely-used CRISPR/Cas systems (CRISPR/Cas9, CRISPR/Cas12a, and CRISPR/Cas13), and elucidated their biological significance and the principles of action. We also reviewed the recent studies on the applications of CRISPR/Cas systems in pathogen detection, and analyzed the characteristics and possible defects of different detection systems in practical applications. This review aims to provide more constructive advice on developing adaptable and efficient CRISPR/Cas-based detection methods for different pathogens in various practical scenarios. |
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| ISSN: | 1008-9209 2097-5155 |