Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally

Sperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims...

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Main Authors: Enni Mutiati, Sri Rahayu, Gatot Ciptadi, Moch. Nasich
Format: Article
Language:English
Published: University of Brawijaya 2016-06-01
Series:Journal of Experimental Life Science
Subjects:
Online Access:https://jels.ub.ac.id/index.php/jels/article/view/180
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author Enni Mutiati
Sri Rahayu
Gatot Ciptadi
Moch. Nasich
author_facet Enni Mutiati
Sri Rahayu
Gatot Ciptadi
Moch. Nasich
author_sort Enni Mutiati
collection DOAJ
description Sperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims to understand the influence of Crude Sperm Extract (CSE) to mice (Mus musculus) estrous cycle. 16 fertile mices strain Balb-C aged 3-4 months, weighed 20-30 g was divided into 4 groups. P0 is a control group injected by PBS, group P1, P2, and P3 injected by CSE with 1.5 mg.ml-1, 2 mg.ml-1, and 2.5 mg.ml-1. CSE is injected intraperitoneally during mice's diestrus phase. CSE is injected in day 0, day 12, day 24 and observed daily. The data are descriptively analyzed. The results show that CSE with molecule weight between 26.8-176.8 kDa influences estrous cycle.
format Article
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2338-1655
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publishDate 2016-06-01
publisher University of Brawijaya
record_format Article
series Journal of Experimental Life Science
spelling doaj-art-ff64f75fb0eb4456b928fc1700ba46752025-08-20T01:55:35ZengUniversity of BrawijayaJournal of Experimental Life Science2087-28522338-16552016-06-01611012https://doi.org/10.21776/ub.jels.2016.006.01.03Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected IntraperitoneallyEnni Mutiati0Sri Rahayu1Gatot Ciptadi2Moch. Nasich3Universitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaUniversitas Brawijaya, Malang, IndonesiaSperm protein has an important role in fertilization process. It becomes antigenic when it is injected to body and can increase TNF-α secretion. TNF-α in blood vesssel can inhibit estradiol synthesis. Estradiol has a significant role in repduction cycle, especially in estrous cycle. The study aims to understand the influence of Crude Sperm Extract (CSE) to mice (Mus musculus) estrous cycle. 16 fertile mices strain Balb-C aged 3-4 months, weighed 20-30 g was divided into 4 groups. P0 is a control group injected by PBS, group P1, P2, and P3 injected by CSE with 1.5 mg.ml-1, 2 mg.ml-1, and 2.5 mg.ml-1. CSE is injected intraperitoneally during mice's diestrus phase. CSE is injected in day 0, day 12, day 24 and observed daily. The data are descriptively analyzed. The results show that CSE with molecule weight between 26.8-176.8 kDa influences estrous cycle.https://jels.ub.ac.id/index.php/jels/article/view/180crude sperm extractestrous cyclefolliculogenesismus musculus
spellingShingle Enni Mutiati
Sri Rahayu
Gatot Ciptadi
Moch. Nasich
Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally
Journal of Experimental Life Science
crude sperm extract
estrous cycle
folliculogenesis
mus musculus
title Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally
title_full Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally
title_fullStr Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally
title_full_unstemmed Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally
title_short Estrous Cycle Response in Mice (Mus musculus) with CSE (Crude Sperm Extract) Injected Intraperitoneally
title_sort estrous cycle response in mice mus musculus with cse crude sperm extract injected intraperitoneally
topic crude sperm extract
estrous cycle
folliculogenesis
mus musculus
url https://jels.ub.ac.id/index.php/jels/article/view/180
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AT srirahayu estrouscycleresponseinmicemusmusculuswithcsecrudespermextractinjectedintraperitoneally
AT gatotciptadi estrouscycleresponseinmicemusmusculuswithcsecrudespermextractinjectedintraperitoneally
AT mochnasich estrouscycleresponseinmicemusmusculuswithcsecrudespermextractinjectedintraperitoneally